Yersinia Pseudotuberculosis Infection in the United States

Hubbert, William T.; Petenyi, C. W.; Glasgow, Lowell A.; Uyeda, Charles T.; Creighton, SM

doi:10.4269/ajtmh.1971.20.679

Cited by 39 publications

(3 citation statements)

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“…The pathology observed in the orally infected mouse is very similar to that observed with Y. pseudotuberculosis in guinea pigs (13) and in man (1,5,7,12,22). As in pseudotuberculosis infection, the primary lesions appear as abscesses in the Peyer's patches and caseous necrosis of the mesenteric lymph nodes.…”

Section: Discussionsupporting

confidence: 67%

Pathogenecity of Yersinia enterocolitica for mice

Carter¹

1975

Infect Immun

188

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A laboratory infection of Yersinia enterocolitica in mice which closely resembles the naturally acquired human infection is described Intravenous inoculation of mice with small numbers of Y. enterocolitica gives rise to a systemic, pyogenic infection involving primarily the spleen, liver, and lungs. Massive neutrophil infiltration of these organs occurs early in the infection, eventually leading to large abscesses and pulmonary consolidation. Mice infected intragastrically show neutophil infiltration in the Peyer's patches of the distal ileum less than 24h postinfection. The Peyer's patches are unable to contain the infection which spreads to the mesenteric lymph node, causing large abscesses in the medullary regions. Soon after, the infection becomes systemic with abscesses forming in the liver, spleen, and lungs, and the total peripheral leukocyte count rises dramatically to over 30,000/mm2. A serological response, in the form of agglutinating antibody, begins to appear 2 weeks after infection. Possible causes of death and the usefulness of this infectious disease model are discussed.

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Section: Discussionsupporting

confidence: 67%

Pathogenecity of Yersinia enterocolitica for mice

Carter¹

1975

Infect Immun

188

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“…Yersinia pseudotuberculosis is an oral pathogen that is typically contained within intestinal tissues and gut-associated lymphoid tissues but has the capacity to spread systemically in susceptible individuals (26–29). Following bloodstream access, Y.…”

Section: Introductionmentioning

confidence: 99%

Iron-Sulfur Cluster Repair Contributes to Yersinia pseudotuberculosis Survival within Deep Tissues

et al. 2019

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To successfully colonize host tissues, bacteria must respond to and detoxify many different host-derived antimicrobial compounds, such as nitric oxide (NO). NO has direct antimicrobial activity through attack on iron-sulfur (Fe-S) cluster-containing proteins. NO detoxification plays an important role in promoting bacterial survival, but it remains unclear if repair of Fe-S clusters is also important for bacterial survival within host tissues. Here we show that the Fe-S cluster repair protein YtfE contributes to the survival of Yersinia pseudotuberculosis within the spleen following nitrosative stress. Y. pseudotuberculosis forms clustered centers of replicating bacteria within deep tissues, where peripheral bacteria express the NO-detoxifying gene hmp. ytfE expression also occurred specifically within peripheral cells at the edges of microcolonies. In the absence of ytfE, the area of microcolonies was significantly smaller than that of the wild type (WT), consistent with ytfE contributing to the survival of peripheral cells. The loss of ytfE did not alter the ability of cells to detoxify NO, which occurred within peripheral cells in both WT and ΔytfE microcolonies. In the absence of NO-detoxifying activity by hmp, NO diffused across ΔytfE microcolonies, and there was a significant decrease in the area of microcolonies lacking ytfE, indicating that ytfE also contributes to bacterial survival in the absence of NO detoxification. These results indicate a role for Fe-S cluster repair in the survival of Y. pseudotuberculosis within the spleen and suggest that extracellular bacteria may rely on this pathway for survival within host tissues.

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“…Y. pseudotuberculosis (Yptb) is closely related to Y. pestis, as Y. pestis evolved from a Yptb ancestor (Achtman et al, 1999;Chain et al, 2004). Yptb is an enteric pathogen that occasionally causes outbreaks of human infection through contaminated produce, although human infection typically results in non-life-threatening, self-limiting gastroenteritis or mesenteric adenitis (Hubbert, Petenyi, Glasgow, Uyeda, & Creighton, 1971;Knapp, 1958;Paff, Triplett, & Saari, 1976). Yptb is a natural pathogen of rodents and is commonly associated with livestock and different wild animal populations (Chlebicz & Slizewska, 2018;Syczylo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Yersinia pseudotuberculosis: Cultivation, Storage, and Methods for Introducing DNA

Davidson

Davis

2020

CP Microbiology

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Yersinia pseudotuberculosis has been studied for many decades, and research on this microbe has taught us a great deal about host-pathogen interactions, bacterial manipulation of host cells, virulence factors, and the evolution of pathogens. This microbe should not be cultivated at 37°C because this is a trigger that the bacterium uses to sense its presence within a mammalian host and results in expression of genes necessary to colonize a mammalian host. Prolonged growth at this temperature can result in accumulation of mutations that reduce the virulence of the strain, so all protocols need to be modified for growth at room temperature, or 26°C. This article describes protocols for cultivating this microbe and for its long-term storage and its genetic manipulation by transformation and conjugation.

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Yersinia Pseudotuberculosis Infection in the United States

Cited by 39 publications

References 0 publications

Pathogenecity of Yersinia enterocolitica for mice

Pathogenecity of Yersinia enterocolitica for mice

Iron-Sulfur Cluster Repair Contributes to Yersinia pseudotuberculosis Survival within Deep Tissues

Yersinia pseudotuberculosis: Cultivation, Storage, and Methods for Introducing DNA

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