Yersinia ruckeri, an unusual microorganism isolated from a human wound infection

Keukeleire, Steven De; Bel, Annelies De; Jansen, Yanina; Janssens, Michéle; Wauters, Georges; Piérard, Denis

doi:10.1002/nmi2.56

Cited by 22 publications

(16 citation statements)

References 12 publications

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“…In our studies, we used Y. ruckeri isolated from an infected rainbow trout in Idaho, USA. This strain is identical to Y. ruckeri recently isolated from a wound infection of a 16-year-old male patient in Belgium 32 . We purified the Afp18 G protein in E. coli and microinjected the recombinant protein into zebrafish zygotes at one-cell stage.…”

Section: Resultssupporting

confidence: 72%

Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos

et al. 2015

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Yersinia species cause zoonotic infections, including enterocolitis and plague. Here we studied Yersinia ruckeri antifeeding prophage 18 (Afp18), the toxin component of the phage tail-derived protein translocation system Afp, which causes enteric redmouth disease in salmonid fish species. Here we show that microinjection of the glycosyltransferase domain Afp18G into zebrafish embryos blocks cytokinesis, actin-dependent motility and cell blebbing, eventually abrogating gastrulation. In zebrafish ZF4 cells, Afp18G depolymerizes actin stress fibres by mono-O-GlcNAcylation of RhoA at tyrosine-34; thereby Afp18G inhibits RhoA activation by guanine nucleotide exchange factors, and blocks RhoA, but not Rac and Cdc42 downstream signalling. The crystal structure of tyrosine-GlcNAcylated RhoA reveals an open conformation of the effector loop distinct from recently described structures of GDP- or GTP-bound RhoA. Unravelling of the molecular mechanism of the toxin component Afp18 as glycosyltransferase opens new perspectives in studies of phage tail-derived protein translocation systems, which are preserved from archaea to human pathogenic prokaryotes.

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Section: Resultssupporting

confidence: 72%

Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos

et al. 2015

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“…Y. ruckeri causes septicaemia and haemorrhages leading to high levels of mortality in infected fish [1,2]. The bacterium also has zoonotic potential and has been associated with topical infections in humans [4]. Like several other members of the genus Yersinia, Y. ruckeri has demonstrated the ability to invade non-professional phagocytic cells [5,6], allowing the bacterium to access restricted nutrients and protecting it from the immune system.…”

Section: Introduction Methods and Resultsmentioning

confidence: 99%

Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri

Menanteau–Ledouble

Schachner

Lawrence

et al. 2020

Vet Res

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Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin assay to quantify the effect of silencing on the cells' susceptibility to infection and found that silencing of 18 out of 20 genes significantly reduced the number of Y. ruckeri recovered. These findings improve our understanding of the infection process by Y. ruckeri and of the interactions between this bacterial pathogen and host cells.

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“…It is associated with acute outbreaks, especially in younger fish, and causes septicaemia and haemorrhages leading to high levels of mortality in infected fish (1–3), especially at temperature between 18 and 20°C. The bacterium has zoonotic potential and has been associated with topical infections in humans (4). Like several other enterobacteriaceae including other members of the genus Yersinia, Y. ruckeri has demonstrated the ability to survive within macrophages (5) as well as invade non-professional phagocytic cells (6, 7).…”

Section: Introductionmentioning

confidence: 99%

Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 toYersinia ruckeri

Menanteau–Ledouble

Schachner

Lawrence

et al. 2019

Preprint

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AbstractBacterial pathogens are known to co-opt mechanisms of the host cells’ physiology to gain intracellular entrance. Among the facultative intracellular bacteria is Yersinia ruckeri, an enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamycin assay to quantify the effect of this silencing on the susceptibility of the cells to infection. It was found that silencing of 16 out of 20 genes significantly reduced the number of Y. ruckeri recovered at the end of the gentamycin assay. Among the genes with the strongest impact were Rab1A, actin and Rac1, which is consistent with our previous findings that N-acetylcysteine, a chemical inhibitor of Rac1, completely prevented invasion of cells by Y. ruckeri. Conversely, silencing of the Rho GTPase activating protein had no statistically significant effect, possibly because Y. ruckeri, like some other members of the Yersinia genus is able to activate Rho GTPase directly. Similarly, the effect of silencing E-cadherin was not statistically significant, suggesting that this might not be a target for the adhesion molecules of Y. ruckeri. Taken together, these findings improve our understanding of the infection process by Y. ruckeri and of the interactions between this bacterial pathogen and host cells.ImportanceIntracellular invasiveness is a mean for bacterial pathogen to gain shelter from the immune system as well as access nutrients. The enterobacterium Y. ruckeri is well characterised as a facultative intracellular pathogen. However, the mechanisms of invasion scrutiny. Investigations have mostly focused on the bacterial virulence rather than on the host’s mechanisms hicjacked during invasion. The present findings therefore allow us to better understand the interaction between this important potentially zoonotic pathogen of fish and host cells in vitro.

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Yersinia ruckeri, an unusual microorganism isolated from a human wound infection

Abstract: We report the first documented case of Yersinia ruckeri isolated from a wound infection, in a 16-year-old male after hitting a stone while paddling in a river.

Cited by 22 publications

References 12 publications

Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos

Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos

Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri

Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 toYersinia ruckeri

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