Yersinia spp. in foods and related environments

Greenwood, M.; Hooper, W. L.

doi:10.1016/0740-0020(85)90007-3

Cited by 25 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this group also contains the largest proportion of apparently symptomless excreters. It has been shown (Greenwood and Hooper, 1985) that pasteurised milk often contains Yersinia organisms. The greatest consumption of milk occurs in this age group and may account partly for this frequency of isolation.…”

Section: Discussionmentioning

confidence: 99%

“…Specimens were also inoculated into 10 ml of buffered peptone water (BPW; Oxoid CM509) containing peptone 1%; this was incubated at 4°C for 17-21 days before subculture to CIN agar. All CIN agar plates were incubated at 30°C for 20-24 h. Suspect colonies were identified by methods described elsewhere (Greenwood and Hooper, 1985). Briefly, isolates were screened with Triple Sugar Iron (TSI) agar (Oxoid) and urea broth.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Human carriage of Yersinia spp.

Greenwood

Hooper

1987

Journal of Medical Microbiology

View full text Add to dashboard Cite

Summary. Examination of faecal specimens for the presence of Yersinia spp. during a 1 -year period yielded isolations from 3.5% of patients. Salmonella spp., Campylobacter spp. and Shigella spp. were isolated from 2.5%, 2.8% and 0.34% of patients respectively. Most isolates belonged to Y . enterocolitica biotype 1 (53%) and Y . frederikseni (39%). The most frequently encountered serotypable strains were serotypes 0 5,27 and 0 6,30. Serotype 0 3, the commonly recognised pathogenic strain, was not isolated in this survey. A strong association between Yersinia excretion and the age group 1-14 years was demonstrated. Although biotype-1 strains and Y. frederikseni have not previously been thought to be pathogenic, clinical significance could be attributed to the presence of Yersinia spp. in almost 90% of patients aged 15 years or more, and in over 50% of patients in the younger age groups.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Human carriage of Yersinia spp.

Greenwood

Hooper

1987

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Samples were also enriched in tris-buffered peptone water pH (TPW) [4], incubated at 14 0C and subeultured to CIN agar after 1-3 and 4-6 days as part of a comparative survey. Suspect colonies were examined for their reaction in triple sugar iron agar (TSI; Oxoid), presence of urease activity, and absence of motility at 37°C [5]. Presumptive strains of yersinia were sent to the reference facility at the Public Health laboratory, Leicester, for confirmation, biotyping according to Bercovier and Mollaret [6] and serotyping by the methods of Wauters [7,8].…”

Section: Methodsmentioning

confidence: 99%

Excretion ofYersiniaspp. associated with consumption of pasteurized milk

Greenwood

Hooper

1990

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYYersinia enterocolitica biotype 1, serotype O.10K was isolated from 19 patients in the paediatric wards of a district general hospital over a period of 3 months. Fifteen cases were patients on the medical ward. Shortly afterwards. Y. enterocolitica biotype 1, serotype 0.6,30 was isolated from a further 17 patients on this ward in 1 month. The same serotypes of Y. enterocolitica were isolated from the pasteurized milk supplied to the ward. Epidemiological evidence indicated that contaminated pasteurized milk was the source of the yersinia organisms exereted by the patients.

show abstract

“…Suspect colonies obtained on CIN agar after incubation for 20-24 h at 30°C were tested by methods previously described [1]. Presumptive strains of yersinia were sent to the reference facility at the Public Health Laboratory, Leicester for confirmation and biotyping according to Bercovier and Mollaret [7], and serotyping by Wauters' scheme [8,9].…”

Section: Microbiological Testing Milk Samplesmentioning

confidence: 99%

The source ofYersiniaspp. in pasteurized milk: an investigation at a dairy

1990

View full text Add to dashboard Cite

SUMMARYPasteurized bottled milk supplied by a single dairy was frequently found to be contaminated with Yersinia spp. Investigations were carried out at the dairy in an effort to pinpoint the source of these organisms. Viable counts obtained from milk bottle rinses indicated that bottle washing was often unsatisfactory, and on one occasion Y. frederiksenii was isolated from the pooled rinse water of six bottles. Samples of milk were taken on arrival at the dairy and at various stages following pasteurization. Heat resistance tests carried out on strains of yersinia isolated from pasteurized milk indicated that they would not survive the pasteurization process. However two strains of yersinia were isolated from a sample of milk taken immediately after pasteurization but before bottling. The thermograph indicated that the ti'me/temperature conditions applied during pasteurization were adequate. The presence of yersinia strains in the milk at this stage therefore suggests that undetectable levels of raw milk were being allowed to contaminate the pasteurized milk. The absence of yersinia in cartoned samples produced on the same day as contaminated bottled samples indicated that environmental contamination of the bottle filler valve also may have occurred at times. Results of this investigation indicate that increased vigilance is required to ensure proper operation of pasteurizers and bottle washers.

show abstract

Yersinia spp. in foods and related environments

Cited by 25 publications

References 23 publications

Human carriage of Yersinia spp.

Human carriage of Yersinia spp.

Excretion ofYersiniaspp. associated with consumption of pasteurized milk

The source ofYersiniaspp. in pasteurized milk: an investigation at a dairy

Contact Info

Product

Resources

About