Lavender (Lavandula officinalis L) has high economic and medicinal value. The current experiment was carried out during the two successive seasons 2018/2019 and 2019/2020. It aimed to investigate the effect of compost, biofertilizers (Azospirillum spp., Azotobacter spp.), mixtures of them, and active dry yeast on vegetative and chemical composition of lavender herbs cultivated on plastic pots filled with sandy soil. Fertilizers were added to the plant roots three times during the growing seasons. Growth and yield characteristics were quantitatively assessed and were compared to each other. Lavender essential oil percentage was determined from the fresh herb, as well as the oil constituents to assess the oil quality. Moreover, the study was tailed by an economic assay to determine the study feasibility. Results indicated that application of biofertilizers significantly increased plant height, number of branches, fresh weight, dry weight, yield, % essential oils, N, P, K%, crude protein content, total pigment, antioxidant, flavonoid, phenol, total carbohydrates, and free amino acids contents compared with the control treatment. Mixtures of bacteria (Azotobacter, Azospirillum) treatments were the superior in all measured variables. It also increased the percentage of essential oils. The main essential oils (EO) constituents were linalool, linalyl acetate, borneol, terpinen4-ol, and 1,8-cineole. From the economic point of view, also the biofertilizer treatments were feasible, this could result in environmentally safe plants to minimize the hazards of pollution caused by using mineral fertilizers. The economic evaluation indicated that mixtures of bacteria resulted in the highest value of net return for lavender plants.