The LED artificial light has been widely applied for greenhouse and indoor plantation. The artificial light generates sole red per blue (R:B) light ratio during plant cultivation from seedling through the fruiting period. The aim of this study is to investigate the fruit quality and the yield of Sida tomatoes under three specific spectra of LED light at different R:B ratios in comparison with the tomato yield and fruit quality under sole R:B light ratio. The experiments were divided into two groups. The 1 st experimental group involved applying the LED light at R25:B75R for the seedling stage, R50:B50 for the vegetative growth, and R75:B25R for the fruit stage. The second experimental group involved applying the LED light at R63:B37 for the seedling, vegetative growth, and fruiting stages. Independent t-test was applied to determine the mean difference of the plant height, stem diameter, fruit yield, fruit weight, fruit size, and TSS. The experimental period was for 115 days. The results confirmed that the three specific spectra of LED light for each growth stage could promote the highest fruit weight, fruit yield, marketable fruit weight, and marketable fruit yield when compared to Sida tomatoes treated with one constant spectrum. Moreover, the level of total soluble sugar is also the highest. Sida tomatoes treated with three specific spectra of LED light has contributed to early and increased fruit yield. This method could be applied to produce high quality tomatoes and yield for the indoor plantation system.