The objective of this study was to determine the effect of nitrogen fertilization rate on growth and quality of leafy lettuce grown during the winter season in non-circulating hydroponic system. Plants were subjected to seven nitrogen (N) concentrations, i.e. 0, 30, 60, 90, 120, 150 and 180 mg L -1 N using ammonium nitrate. Nitrogen treatments did not have a significant effect on leaf fresh and dry mass, root fresh and dry mass, number leaves and leaf area. Leaf ascorbic Downloaded by [University of California Santa Barbara] at 00:59 15 June 2016 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 2 acid and total phenolic content, and antioxidant capacity peaked at 100 and 120 mg L -1 N,whereas leaf chlorophyll concentration linearly increased with increasing N application. The results indicate that a solution N concentration of 100 and 120 mg L -1 may be sufficient to improve growth, yield and quality parameters of leafy lettuce grown in non-circulating hydroponic system.