Yield coefficients in dependence on milieu conditions and cell states. I. Synchronized batch growth of a yeast

Bley, Th.; Heinritz, B.; Steudel, A.; Stichel, Ellen Van; Glombitza, F.; Babel, Wolfgang

doi:10.1002/jobm.3630200407

Cited by 20 publications

(2 citation statements)

References 9 publications

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“…In contrast to this, the budding-cell state is determinate and independent of the carbon-substrate supply. These results are in agreement with experiments of MEYENBURG 1521, GILLIES et al [76], BANKS [77l and BLEY et al [78]. They can be explained by -the formation of ATP and polyphosphates in the single-cell state with an efficiency depending on the carbon-substrate concentration and the kind of limitation The results of the continuous phased cultivation indicate that cell-mass production should be optimized by adapting the environmental factors, especially the carbon-substrate supply to the repetitive yeast-cell states reflected by the states of synchronized yeast-cell population.…”

Section: Deduction Of the Dynamic Process Control Concept In Experimentssupporting

confidence: 83%

Dynamic process control versus steady‐;state fermentation

Zentgraf¹,

Rogge²,

Bley³

1992

Acta Biotechnologica

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Process control strategies, e.g. the steady-state fermation, which do not consider the cell-state distribution of cell populations, cause a decrease of yield coefficient connected to an increase of heat-production coefficient. Contrary to this, the dynamic process control adapts the carbon-substrate supply to the different repetitive cell-population states during fermentation. In order to recognize the different states, the cell population is synchronized. The optimum period of changing carbon-substrate supply was found to be dependent on the celI-doubling time. Dynamic process control causes a higher yield coefticient as well as a lower heat-production coeficient compared to the steady-state fermentation.

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Section: Deduction Of the Dynamic Process Control Concept In Experimentssupporting

confidence: 83%

Dynamic process control versus steady‐;state fermentation

Zentgraf¹,

Rogge²,

Bley³

1992

Acta Biotechnologica

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“…Zur umfassenden Charakterisierung ist aber die Kopplung von Stoffbilanzen mit Energiebilanzen notwendig (PAYNE 1970, BABEL 1979, 1980, BLEY et al 1980 Eiir die Beurteilung der Effektivitat der mikrobiellen Substratwandlung ist neben den angefiihrten GroGen der energetische Wirkungsgrad von Bedeutung (PRIGOGINE 1969, HEINRITZ 1978, BABEL 1979, GLOMBITZA u. HEINRITZ 1979, MINKEVICH 1980. Energetische Wirkungsgrade der mikrobiellen Verwertung verschiedener Substrate sind unter Verwendung der freien Enthalpie, die die maximale Nutzarheit angibt, und der Enthalpie, die gleich der Warnietonung unter isobaren und isothernien Bedingcingen ist, als Bilanzgrofien berechenbar.…”

Section: Theoretische Untersuchungenunclassified

Theoretische Bestimmung energetischer Wirkungsgrade der mikrobiellen Kohlenstoffsubstratwandlung und Vergleich mit experimentellen Werten an Phasenkulturen

Heinritz¹,

Stichel²,

Rogge³

et al. 1982

J of Basic Microbiology

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The phenomenological description of microbial substrate conversion is possible by connecting energy and material balance equations.The change of free enthalpy of microbial substrate conversion was determined on the basis of exact material balance equations. A simultaneous determination of heat production allowed to calculate the energetic efficiency of microbial growth processes. I n addition to this the change of free enthalpy by microbial substrate conversion was standardized by the free standard formation enthalpy of biomass. Both quantities were used for comparing microbial combustion of several substrates into biomass. Comparable with the concept of substrate combustion enthalpy standardized by the combustion enthalpy of biomass an optimal .strategy of mixing different substrates can be deduced from energy and material balance equaticns on the basis of theoretic consumption coefficients.Besides the energetic efficiency the binding state of the substrate is important in the search for optimal substrate mixing ratios. This could be shown by experimental work. Thus, the fermentation systems sucrose/yeast, paraffinlyeast, and sucrose/paraffin/yeast were studied using synchronous populations produced by the method of phased cultures. The investigations of real fermentation systems confirm the theoretical predictions on special conditions.

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Heat flow measurements in aerobic microbial growth processes with a nonisothermal calorimeter operating directly in the fermenter

Heinritz¹,

Stoll²,

Glombitza³

1983

Acta Biotechnologica

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A nonisothermal flow cdorimeter operating directly in the fermenter was used for heat flow meitsurements of aerobic microbial growth processes with high biomass productivities. The measuring nrrangement.makes i t possible to describe transitional stages of aerobic yeast cell growth by thc ratio of heat production to oxygen consumption (oxy-caloric coefficient). The oxy-caloric coefficient was not constant under the described conditions. The results refer to the existence of an additional encrgy-delivering mechanism in microbial systems with aerobic carbon source utilization. The mechanism c:tn involve polyphosphnte bond division coupled to biomass synthesis. ZusamnicnfassungEin im Fermentor arbeitendes anisothermes DurchfluDkalorimeter wurde zu WiirmefluBiuessungen an seroben mikrobiellen Wachstumsprozessen bei hohen Biomassebildungsraten angeaendet. Die MeBanordnung erlaubt die Beschreibung des ubergangsverhaltens aerober Wachstumsvorgiinge durch die Ermittlung des Verhiiltnisses von Warmeproduktion und Sauerstoffverbrauch (oxy-kalorischer Koeffizient). Dieser Koeffizient ist unter den beschriebenen Bedingungen keine Konstante. Die experimentellen Ergebnisse deuten auf die Existenz eines zusiitzlichen energieliefernden Mechanismus in mikrobiellen Systemen, in denen die Kohlenstoff-nnd Energiequelle oxydntiv abgebaut wird. Dieser Mechanismus kann eine enge Kopplung der Spaltung von Polyphosphatbindungen und der Biomassesynthese enthnlten.

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Yield coefficients in dependence on milieu conditions and cell states. I. Synchronized batch growth of a yeast

Cited by 20 publications

References 9 publications

Dynamic process control versus steady‐;state fermentation

Dynamic process control versus steady‐;state fermentation

Theoretische Bestimmung energetischer Wirkungsgrade der mikrobiellen Kohlenstoffsubstratwandlung und Vergleich mit experimentellen Werten an Phasenkulturen

Heat flow measurements in aerobic microbial growth processes with a nonisothermal calorimeter operating directly in the fermenter

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