Yijin-Tang Attenuates Cigarette Smoke and Lipopolysaccharide-Induced Chronic Obstructive Pulmonary Disease in Mice

Abstract

Background. Chronic obstructive pulmonary disease (COPD) refers to a lung disorder associated with symptoms of dyspnea, cough, and sputum production. Traditionally, Yijin-tang (YJT), a mixture of Pinellia ternate, Poria cocos, ginger, Chinese liquorice, and tangerine peel, has been prescribed for the treatment of respiratory system diseases caused by dampness phlegm. This experiment investigated the therapeutic effect of YJT in a mouse model of cigarette smoke (CS)- and lipopolysaccharide (LPS)-induced COPD. Methods. COPD was induced by exposing mice to CS for 1 hour per day for 8 weeks, with intranasal delivery of LPS on weeks 1, 3, 5, and 7. YJT was administered at doses of 100 and 200 mg/kg 1 hour before CS exposure for the last 4 weeks. Results. YJT significantly suppressed CS- and LPS-induced increases in inflammatory cell counts and reduced interleukin-1 beta (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) levels in bronchoalveolar lavage fluid (BALF) and lung tissue. In addition, YJT not only decreased airway wall thickness, average alveolar intercept, and lung fibrosis, but it also suppressed the expression of matrix metallopeptidase (MMP)-7, MMP-9, and transforming growth factor-B (TGF-β) and collagen deposition. Moreover, YJT suppressed phosphorylation of nuclear factor-kappa B (NF-κB) as well as expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Conclusion. Collectively, our findings show that YJT attenuates respiratory inflammation and airway remodeling caused by CS and LPS exposure; therefore, therapeutic applications in COPD can be considered.

Anti-Inflammatory and Anti-Airway Remodeling Activities of Jakyakgamcho-Tang in a Mouse Model of COPD

“…According to the above description, the level of inflammatory cell infiltration was graded from 0 (absent) to 4 (severe) [19]. The mean linear intercept (MLI) was calculated from 5 images of 4 animals in each group at random to investigate lung changes [20]. At 20× magnification, five equally spaced horizontal lines were examined.…”

Study on Antioxidant Activity and Standardization of Craniosacral Pharmacopuncture

“…이진탕은 담음(痰飮)을 제거 하는 데에 사용되는 처방이다 10,11) . 소화기 질환 12) , 역류 성 식도염 13) 등의 연구들이 보고되어 있으며 최근 장내미 생물 조절을 통해 지방간 및 당뇨병을 개선하고 14) 만성 폐쇄성 폐질환에도 효능 15) 이 있다고 하였다. 자하거는 월경통 16) , 골다공증 17) , 골관절염 18) 등에 사용되며 특히 만성피로 같은 허로(虛勞)증에 다빈도로 처방된다 19,20) .…”