Yin Chen Hao Tang, a Chinese prescription, inhibits both herpes simplex virus type-1 and type-2 infections in vitro

Cheng, Hua; Lin, Liang; Huang, Hsin Hsin; Yang, Chien Min; Lin, Chih‐Hsueh

doi:10.1016/j.antiviral.2007.08.012

Cited by 40 publications

(25 citation statements)

References 22 publications

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“…Many antiviral agents have been tested for their effect on human herpes simplex viruses, the counterparts of PrV in the subfamily Alphaherpesvirinae in Herpesviridae family (De Clercq, 2004;Naesens and De Clercq, 2001;Sergerie and Boivin, 2008;Cheng et al, 2008). Due to the potential threat of PrV infection to the animal husbandry, it is necessary to develop effective antiviral agents against PrV infection.…”

Section: Discussionmentioning

confidence: 99%

Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus

2010

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Diammonium glycyrrhizin (DG), a salt from glycyrrhizinate (GL) that is a major active component of licorice root extract with various pharmacological activities was investigated for its inhibitory effect on pseudorabies virus (PrV) infection. In parallel, lithium chloride (LiCl), a chemical reagent with potential antiviral activity was compared with DG for their inhibitory ability against PrV infection in vitro. Virus plaque-reduction assays, PCR and RT-PCR analysis indicated that both drugs inhibited cell infection by PrV. Moreover, addition of the drugs resulted in fewer apoptotic cells during PrV infection.

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Section: Discussionmentioning

confidence: 99%

Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus

2010

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“…The cell monolayer was then stained with 1% crystal violet. The fraction of percent inhibition in inhibiting HSV infection was determined, and the minimal concentration that inhibited the formation of virus plaque number by 50% (IC 50 ) was calculated [49]. …”

Section: Methodsmentioning

confidence: 99%

“…The time-of-addition effect of excoecarianin was examined according to the previously described procedures with some modifications [49]. Briefly, Vero cells were seeded onto 24-well culture plates (Falcon, BD Biosciences, USA) at a density of 1 × 10 5 cells per well and incubated for 48 h to reach at least 95% confluency.…”

Section: Methodsmentioning

confidence: 99%

Excoecarianin, Isolated from Phyllanthus urinaria Linnea, Inhibits Herpes Simplex Virus Type 2 Infection through Inactivation of Viral Particles

Cheng

Yang

Lin

et al. 2011

Evidence-Based Complementary and Alternative Medicine

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Phyllanthus urinaria Linnea (Euphorbiaceae) is one of the traditional medicinal plants widely used by oriental people to treat various diseases. We have previously demonstrated that the acetone extract of P. urinaria inhibits herpes simplex virus type 2 (HSV-2) but not HSV-1 infection. In a continuing effort to clarify the antiviral mechanisms of P. urinaria, we isolated the pure compound excoecarianin from the whole plant of P. urinaria through acetone extraction, and investigated its anti-HSV-1 and HSV-2 activities. Our results indicated that excoecarianin protected Vero cells from HSV-2 but not HSV-1 infection, and its 50% inhibitory concentration (IC50) was 1.4 ± 0.1 μM. The antiviral effective concentration of excoecarianin did not affect the viability or the morphology of Vero cells. Although excoecarianin inhibited HSV-2 infection, the inhibitory effect, however, was most prominent when excoecarianin was concurrently added with the virus. Pretreatment of Vero cells with excoecarianin with removal of the drug prior to infection did not yield any antiviral effects, and the same observation was made for post viral entry treatment. Subsequent studies revealed that excoecarianin inactivated HSV-2 virus particles to prevent viral infection. A synergistic antiviral effect against HSV-2 was also observed when Vero cells were treated with a combination of acyclovir (ACV) and excoecarianin. These results suggested that excoecarianin merits to be further explored as an entry inhibitor against HSV-2 and could potentially be investigated for combinatorial drug treatment with nucleoside analogues such as ACV in therapeutic management of HSV-2 infection.

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“…For instance, ent-epiafzelechin-(4a→8)-epiafzele chin, extracted from Cassia javanica, inhibits HSV-2 replication; the herbal prescriptions Long-Dan-Xie-Gan-Tan (龍膽瀉肝湯 Lóng Dǎn Xiè Gān Tāng) and Yin-Chen-Hao-Tang (茵陳蒿湯 Yīn Chén Hāo Tang) both possess broad efficiency in diminishing HSV-1 and HSV-2 infectivity; hippomanin A, geraniin, 1,3,4,6-tetra-O-galloyl-beta-d-glucose, and excoecarianin isolated from Phyllanthus urinaria (葉下珠 Yè Xià Zhū) can potently impede HSV infection. [72][73][74][75][76][77] In addition, we have also identified the hydrolyzable tannins chebulagic acid and punicalagin as cell surface glycosaminoglycan (GAG) competitors that can inhibit HSV-1 entry and cell-to-cell spread. [78] HSV-1 and also a multitude of viruses employ GAGs as initial attachment receptors during infection of their host cell.…”

Section: Herpes Simplex Virusmentioning

confidence: 99%

Antiviral Natural Products and Herbal Medicines

Lin

Hsu

Lin

2014

Journal of Traditional and Complementary Medicine

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453

355

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Viral infections play an important role in human diseases, and recent outbreaks in the advent of globalization and ease of travel have underscored their prevention as a critical issue in safeguarding public health. Despite the progress made in immunization and drug development, many viruses lack preventive vaccines and efficient antiviral therapies, which are often beset by the generation of viral escape mutants. Thus, identifying novel antiviral drugs is of critical importance and natural products are an excellent source for such discoveries. In this mini-review, we summarize the antiviral effects reported for several natural products and herbal medicines.

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Yin Chen Hao Tang, a Chinese prescription, inhibits both herpes simplex virus type-1 and type-2 infections in vitro

Cited by 40 publications

References 22 publications

Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus

Antiviral effect of diammonium glycyrrhizinate and lithium chloride on cell infection by pseudorabies herpesvirus

Excoecarianin, Isolated from Phyllanthus urinaria Linnea, Inhibits Herpes Simplex Virus Type 2 Infection through Inactivation of Viral Particles

Antiviral Natural Products and Herbal Medicines

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