Yin Yang-1(YY-1) expression in idiopathic pulmonary fibrosis

Margaritopoulos, G; Αντωνίου, Κατερίνα; Soufla, Giannoula; Vassalou, Evi; Spandidos, Demetrios Α.; Siafakas, Nikos M.

doi:10.3109/10799893.2011.557735

Cited by 3 publications

(4 citation statements)

References 34 publications

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“…Certain studies have suggested a link between IPF and lung cancer through different pathogenetic mechanisms, such as viral implications, inflammation, coagulation, dysregulated apoptosis, focal hypoxia, activation of oncogenes, genetics and the accumulation of myofibroblasts, as well as extracellular matrix accumulation (21,22). However, diversities in the expression of molecular pathways have also been recently described by ours (14,23) and other study groups (4–6). Previous studies have demonstrated that mutations in telomerase h-TERT and h-TERC genes cause a shortening of telomere lengths, and account for approximately 10% of familial IPF cases (24–26).…”

Section: Discussionsupporting

confidence: 84%

“…Control lung tissue specimens were obtained from patients undergoing lobectomy or pneumonectomy for bronchogenic carcinoma, at a macroscopically healthy site distant from the malignancy. The samples were further verified histologically as free of malignancy before being classified as control samples, as previously described by ours and other groups (14,15). The BALF samples were obtained from 23 patients with IPF, 31 patients with NSCLC and 12 controls.…”

Section: Methodsmentioning

confidence: 99%

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Differential telomerase expression in idiopathic pulmonary fibrosis and non-small cell lung cancer

et al. 2013

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Telomerase is a reverse transcriptase ribonucleo-protein (h-TERT) that synthesizes telomeric repeats using its RNA component (h-TERC) as a template. Telomerase dysfunction has been associated with both fibrogenesis and carcinogenesis. In this study, we aimed to evaluate the telomerase mRNA expression levels of both subunits (h-TERT and h-TERC) in lung tissue and bronchoalveolar lavage fluid (BALF) from patients with idiopathic pulmonary fibrosis (IPF) and non-small cell lung cancer (NSCLC), since there are indications of common pathogenetic pathways in these diseases. We prospectively examined lung tissue samples from 29 patients with IPF, 10 patients with NSCLC and 21 controls. Furthermore, we examined BALF samples from 31 patients with NSCLC, 23 patients with IPF and 12 control subjects. The mRNA expression for both h-TERT and h-TERC was measured by real-time RT-PCR. In the lung tissue samples, both h-TERT and h-TERC mRNA expression levels varied among the 3 groups (p=0.036 and p=0.002, respectively). h-TERT mRNA levels in the patients with IPF were lower compared with those in the controls (p=0.009) and patients with NSCLC (p=0.004). h-TERC mRNA levels in the patients with IPF were lower compared with those in the controls (p=0.0005) and patients with NSCLC (p=0.0004). In the BALF samples, h-TERT mRNA expression levels varied among the groups (p=0.012). More specifically, h-TERT mRNA levels in the patients with IPF were higher compared with those in the controls (p=0.03) and patients with NSCLC (p=0.007). The attenuation of telomerase gene expression in IPF in comparison to lung cancer suggests a differential role of this regulatory gene in fibrogenesis and carcinogenesis. Further functional studies are required in order to further elucidate the role of telomerase in these devastating diseases.

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Section: Discussionsupporting

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Differential telomerase expression in idiopathic pulmonary fibrosis and non-small cell lung cancer

et al. 2013

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“…RNA expression was measured using a real-time RT-PCR assay with SYBR-Green I. Primers were designed to span introns. β-actin was used as the internal control to normalize VEGF, FLT-1, Ang-1, Ang-2, Tie-2A, Tie-2B, SDF-1α, SDF-1β, CXCR4 and YY1 (15)(16)(17)(18)(19) (Table I). In particular, 1 µl cDNA from all patient and control samples was amplified in a PCR reaction containing 2X Brilliant SYBR-Green I QPCR Master Mix, 300 nM of each primer and 30 µM ROX passive reference dye, in a final volume of 20 µl.…”

Section: Rna Isolation and Rt-pcrmentioning

confidence: 99%

Investigation of angiogenetic pathways in nasal polyposis

et al. 2012

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Abstract. Tissue angiogenesis is a complex phenomenon that results in the growth of new blood vessels from the microcirculation. This process has been known to play a crucial role in tumor growth as well as several benign diseases. The aim of this study was to assess mRNA expression of various angiogenic factors and chemokines in nasal polyps and compare the results to normal nasal mucosa. mRNA expression was measured using real-time RT-PCR for the following angiogenic factors and chemokines: VEGF, VEGFR-1, Ang-1, Ang-2, Tie-2A, Tie-2B, SDF-1α, SDF-1β, CXCR4 and YY1. Biopsy specimens from nasal polyps in the polyposis group and middle turbinates in the control group were studied. A total of 18 nasal polyposis patients were studied and compared to 10 control subjects. Results showed VEGF, VEGFR-1, Ang-1, Ang-2, Tie-2A, Tie-2B, SDF-1α and SDF-1β mRNA expression to be significantly higher in nasal polyposis patients compared to the control group (p<0.05). The findings of this study support the role of angiogenic growth factors in the pathogenenesis of nasal polyposis. Further studies are required to confirm these results and evaluate potential clinical implications. IntroductionNasal polyposis (NP) constitutes a chronic inflammatory disease of the nose and sinuses characterized by the appearance of protruding tissue swellings of the nasal mucosa. These swellings most commonly originate from the middle meatus and anterior ethmoids. On histologic examination, they are typically characterized by proliferation of the epithelial layer, glandular hyperplasia, thickening of the basal membrane, focal fibrosis, edema and cellular infiltration of the stromal layer. Nasal polyps are relatively common in the general population and significantly affect patient quality of life. Despite these facts, etiology of the disease remains largely unknown (1-4).Tissue angiogenesis is a complex phenomenon that results in the growth of new blood vessels from the microcirculation (5-7). This process has been known to play a crucial role in tumor growth, where it is regarded as a prerequisite allowing for the expansion of tumor populations beyond the size restricted by oxygen and nutrient diffusion (6). It also facilitates tumor progression and metastasis by causing degradation of the epithelial basement membrane and providing cancer cells with new capillaries for entering the circulation (7,8).Increasing evidence shows that angiogenesis may also play a critical role in benign disorders (9,10). The phenomenon, appearing in the form of growth of new blood vessels or the enlargement of existing ones, has been strongly associated with airway remode ling in chronic asthma and chronic obstructive pulmonary disease (11,12). In addition, using immunohistochemistry it has been shown that vascular endothelial growth factor (VEGF), possibly the most important driver of angiogenesis, along with its receptors, are strongly expressed in nasal polyp tissues, thus supporting a role in the development of chronic inflammation and edema in this disease (13).Re...

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“…Transforming growth factor (TGF)-b, which is known as a potent stimulator of fibro-production in IPF, stimulates PAR-2 production in human lung fibroblasts and therefore could be closely related to intensifying fibrotic processes [24]. The other novel molecule being overexpressed under TGF-beta stimulation is Ying Yang 1 (YY1), a gene expression regulator, which might also be involved in IPF and lung cancer pathogenesis [25,26].…”

Section: Introductionmentioning

confidence: 98%

IL-4 polymorphisms, HRCT score and lung tissue markers in idiopathic pulmonary fibrosis

et al. 2013

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Yin Yang-1(YY-1) expression in idiopathic pulmonary fibrosis

Abstract: Our results do not support the hypothesis of YY-1 involvement in IPF. However, similar expression of YY-1 gene in two biological samples cannot exclude a possible role of this polymorphic gene in the pathway of IPF. Further studies in a larger scale of patients are needed.

Cited by 3 publications

References 34 publications

Differential telomerase expression in idiopathic pulmonary fibrosis and non-small cell lung cancer

Differential telomerase expression in idiopathic pulmonary fibrosis and non-small cell lung cancer

Investigation of angiogenetic pathways in nasal polyposis

IL-4 polymorphisms, HRCT score and lung tissue markers in idiopathic pulmonary fibrosis

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