Yinchenzhufu decoction protects against alpha-naphthylisothiocyanate-induced acute cholestatic liver injury in mice by ameliorating disordered bile acid homeostasis and inhibiting inflammatory responses

Wang, Guofeng; Li, Yuanyuan; Shi, Rong; Wang, Tianming; Li, Yifei; Li, Wenkai; Zheng, Min; Fan, Feng-Bo; Zou, Juan; Zan, Bin; Wu, Jiasheng

doi:10.1016/j.jep.2020.112672

Cited by 26 publications

(13 citation statements)

References 37 publications

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“…ANIT is a chemical substance that imitates cholestasis in human liver. It is coupled to glutathione in hepatocytes and transported from bile duct outflow transporter, namely, multidrug resistance-associated protein 2 (MRP2) into bile (Wang et al, 2020). Here, ANIT and glutathione resolve, meanwhile, selectively damage bile duct epithelial cells, causing cholangitis and subsequent intrahepatic cholestasis.…”

Section: Exploration Of Preclinical Mechanismmentioning

confidence: 99%

Preclinical evidence of Yinchenhao decoction on cholestasis: A systematic review and meta‐analysisof animal studies

Shi

Wen

Zeng

et al. 2020

Phytotherapy Research

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Cholestasis is an important cause of liver fibrosis and cirrhosis. Yinchenhao decoction has been used as a well‐known traditional Chinese medicine used in the treatment of cholestasis for over 2,000 years. The purpose of this systematic review is to evaluate the preclinical evidence of Yinchenhao decoction on cholestasis models. The following databases were searched from inception to February 2020. Chinese National Knowledge Infrastructure, VIP medicine information system, Wanfang Database, PubMed, Web of Science, Embase and the Cochrane Library were searched. The content concerned Yinchenhao decoction on different animal model experiments for the treatment of cholestasis. The methodological quality of the included studies was assessed based on the SYstematic Review Center for Laboratory animal Experimentation Animal Experiment Bias Risk Assessment Tool. A meta‐analysis was conducted with RevMan 5.3 software according to the Cochrane tool. Nineteen studies on a total of 404 animals were included with five kinds of experimental animal models. The results showed that serum total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin and total bile acid in the group treated with Yinchenhao decoction were significantly lower than those in the model group (P < 0.00001). The alanine aminotransferase (ALT), aspartate aminotransferase and alkaline phosphatase levels in the Yinchenhao decoction group were also significantly reduced (P < 0.00001). The subgroup analysis of the different models showed that Yinchenhao decoction had a significant effect on the bile duct ligation model, and there was a significant reduction in TBIL, DBIL and ALT levels (P < 0.00001) in ANIT‐induced cholestasis. After 24 hours of Yinchenhao decoction treatment, there was no significant difference in TBIL levels (P = 0.34), but after 48 and 72 hours of treatment, the TBIL levels were significantly reduced compared with the model group (P < 0.00001). There was no significant difference in DBIL after 48 hours of administration (P = 0.26), but compared with the model group, Yinchenhao decoction could significantly reduce the DBIL levels after 48 hours of treatment (P < 0.0003). Yinchenhao decoction could significantly reduce the ALT levels after 24, 48 and 72 hours (P < 0.006). Yinchenhao decoction was able to significantly reduce the levels of TBIL, DBIL and ALT on different rat species: Wistar and Sprague Dawley (P = 0.0001; P = 0.0002). The preclinical evidence indicated that Yinchenhao decoction might be a potent and promising agent for cholestasis. Moreover, this conclusion should be further confirmed with more well‐designed researches.

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Section: Exploration Of Preclinical Mechanismmentioning

confidence: 99%

Preclinical evidence of Yinchenhao decoction on cholestasis: A systematic review and meta‐analysisof animal studies

Shi

Wen

Zeng

et al. 2020

Phytotherapy Research

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“…Dysfunction in the formation, secretion, and excretion of bile acids may lead to the accumulation of a large amount of bile acids in the liver, which in turn leads to liver damage (Kong et al, 2013; Liu et al, 2019). It has been reported that the decrease in bile acid content in the serum and liver can be used as a prognostic indicator of cholestatic liver injury, while the increased bile acid content in the bile can reduce the liver toxicity induced by bile acid (Li et al, 2019; Wang et al, 2014, 2017, 2020). Our results were consistent with the observations in a study showing that the Xiaoyan Lidan Formula ameliorates ANIT‐induced intrahepatic cholestatic liver injury in rats by reversing the increase of hepatic bile acids and decreasing serum bile acids levels induced by ANIT (Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Section: Guidelines For the Diagnosis And Early Treatment Of Drug-ind...mentioning

confidence: 99%

Farnesoid X receptor contributes to oleanolic acid‐induced cholestatic liver injury in mice

Hong

Zhou

et al. 2022

J of Applied Toxicology

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Farnesoid X receptor (FXR) is a nuclear receptor involved in the metabolism of bile acid. However, the molecular signaling of FXR in bile acid homeostasis in cholestatic drug-induced liver injury remains unclear. Oleanolic acid (OA), a natural triterpenoid, has been reported to produce evident cholestatic liver injury in mice after a longterm use. The present study aimed to investigate the role of FXR in OA-induced cholestatic liver injury in mice using C57BL/6J (WT) mice and FXR knockout (FXR À/À ) mice. The results showed that a significant alleviation in OA-induced cholestatic liver injury was observed in FXR À/À mice as evidenced by decreases in serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase as well as reduced hepatocyte necrosis. UPLC-MS analysis of bile acids revealed that the contents of bile acids decreased significantly in liver and serum, while increased in the bile in FXR À/À mice compared with in WT mice. In addition, the mRNA expressions of hepatic transporter Bsep, bile acid synthesis enzymes Bacs and Baat, and bile acids detoxifying enzymes Cyp3a11, Cyp2b10, Ephx1, Ugt1a1, and Ugt2b5 were increased in liver tissues of FXR À/À mice treated with OA. Furthermore, the expression of membrane protein BSEP was significantly higher in livers of FXR À/À mice compared with WT mice treated with OA. These results demonstrate that knockout of FXR may alleviate OA-induced cholestatic liver injury in mice by decreasing accumulation of bile acids both in the liver and serum, increasing the export of bile acids via the bile, and by upregulation of bile acids detoxification enzymes.

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“…Studies conducted on Linggui Zhugan decoction ( Supplementary Table S2 ) showed that the prescriptions could significantly improve liver fat content, and Baizhu played a role in invigorating the spleen and dryness of the spleen ( Liu et al, 2013a ). In the Yinchen Zhufu decoction ( Supplementary Table S2 ), Baizhu is an important crude drug that exerts a good protective effect against alpha-naphthylisothiocyanate-induced acute cholestatic liver injury in mice ( Wang GF. et al, 2020 ).…”

Section: Applicationmentioning

confidence: 99%

A Review of the Ethnopharmacology, Phytochemistry, Pharmacology, Application, Quality Control, Processing, Toxicology, and Pharmacokinetics of the Dried Rhizome of Atractylodes macrocephala

Hou

Man

et al. 2021

Front. Pharmacol.

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The product investigated herein is the dried rhizome of Atractylodes macrocephala Koidz. [Asteraceae] (Baizhu), which is also known as Dongbaizhu, Wuzhu, Yuzhu, Zhezhu, and Zhongzhu, among others. It invigorates the spleen, replenishes qi, and removes dampness, diuresis, and hidroschesis, and impacts fetal safety. It is often used for the treatment of diseases such as spleen function deficiency, abdominal distension, diarrhea, sputum, vertigo, edema, fever, and sweating and also aids cessation of minimal vaginal bleeding during pregnancy. In this study, research pertaining to the ethnopharmacology, application, phytochemistry, analytical methods, quality control, processing, pharmacology, toxicology, and pharmacokinetics of Baizhu has been reviewed. Relevant information and data reported for Baizhu were collected from CNKI, VIP, PubMed, Web of Science, scientific databases, Chinese Medicinal Material Encyclopedia, Chinese herbal medicine classics, Chinese medicine dictionary, doctoral and master’s theses, and so on. Baizhu demonstrates diuretic, antidiuretic, anti-inflammatory effects and antitumor function and aids regulation of gastrointestinal function, hypoglycemic effect, analgesic action, protection on the liver ischemia-reperfusion injury (IRI) in rats, inhibition of aromatase, treatment of bone disease, strengthening myocardial contraction ability, detoxification and cholagogic effect, fall hematic fat action, such as the treatment of acute renal injury, and so on. It also can be an anticoagulant, improve the nervous system disease, affect the immune system, and regulate uterine smooth muscle, antioxidation, antiaging, and antibacterial effect. Sesquiterpenoids, triterpenoids, polyacetylenes, phenylpropanoids, coumarins, flavonoids, flavonoid glycosides, steroids, benzoquinones, polysaccharides, and other compounds were isolated from Baizhu. Among them, sesquiterpenoids, polysaccharides, and polyacetylenes are the main components of Baizhu. Baizhu exhibits a wide range of pharmacological effects and constitutes a considerable proportion of the composition of many proprietary crude drugs. It mainly affects the endocrine, nervous, and urinary systems. The presented information suggests that we should focus on the development of new drugs related to Baizhu, including specific components, to achieve a greater therapeutic potential that can be considered to further explore the information related to Baizhu.

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Yinchenzhufu decoction protects against alpha-naphthylisothiocyanate-induced acute cholestatic liver injury in mice by ameliorating disordered bile acid homeostasis and inhibiting inflammatory responses

Cited by 26 publications

References 37 publications

Preclinical evidence of Yinchenhao decoction on cholestasis: A systematic review and meta‐analysisof animal studies

Preclinical evidence of Yinchenhao decoction on cholestasis: A systematic review and meta‐analysisof animal studies

Farnesoid X receptor contributes to oleanolic acid‐induced cholestatic liver injury in mice

A Review of the Ethnopharmacology, Phytochemistry, Pharmacology, Application, Quality Control, Processing, Toxicology, and Pharmacokinetics of the Dried Rhizome of Atractylodes macrocephala

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