YinYang 1 (YY1) and P53 Gene Expression Analysis in Cervical Cancer and Its Relationship with Cancer Staging

Wibisono, Jacobus Jeno; Rauf, Syahrul; Hatta, Mochammad; Natsir, Rosdiana; Massi, Maurizio; Cangara, Muhammad Husni; Masadah, Rina; Pattelongi, Ilham Jaya; Salim, Jonathan

doi:10.13005/bpj/1977

Cited by 1 publication

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“…The mRNA concentration was measured with a QubitTM fluorometer (Invitrogen, Carlsbad, CA, United States) according to the manufacturer's 90 instructions, and the RNA samples were then stored at −80 °C until the next analysis. Examination of expression mRNA gene using the nucleic acid extraction method, expression mRNA gene Treg using the Real-Time Polymerase Chain Reaction (RT PCR) method, calculating the calibration curve with Ct (Cycle threshold) [ [12] , [13] , [14] , [15] ].…”

Section: Methodsmentioning

confidence: 99%

Relationship between expression mRNA gene Treg, Treg, CD4+, and CD8+ protein levels with TST in tuberculosis children: A nested case-control

Farsida

Shabariah

Hatta

et al. 2021

Annals of Medicine and Surgery

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Background The ability of Mycobacterium tuberculosis to survive intracellularly, provides a cellular adaptive immune response played by specific T cells to defend against tuberculosis. The adaptive immune response to Bacillus of Calmette and Guerin (BCG) immunization is responded to by B cells, T Follicular B helper, T regulatory, restriction CD1, CD8 + , CD4 + , Th1, Th2, and Th17. BCG immunization can cause a tuberculin test reaction to being positive. The tuberculin test is a method for diagnosing TB infection and for screening individuals for latent infection and assessing the rate of TB infection in a given population. Methods a nested case-control survey was conducted on patients with a diagnosis of TB and parents 0–18 years of age from 3 hospitals in Indonesia during September–November 2019 with a total sample of 69 people undergoing clinical examinations, supporting and diagnosing subjects, blood sampling 1–2 cc for examination mRNA gene Treg, Treg, CD 4+, and CD 8+, then centrifuged at 3000 rpm for 10 min to support blood cells and serum. Results There was a significant relationship between expression of mRNA gene Treg with TST (p = 0,000), Treg with TST (p = 0,000), and CD4 + with TST (p = 0,000). Meanwhile, CD8 + was not significantly associated with TST (p = 0.118). Conclusions It is necessary to check the expression of mRNA gene Treg, Treg, CD4 + , and CD8 + with more samples to find the mean value that shows the protective value of further TB.

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Section: Methodsmentioning

confidence: 99%