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Mezhlumyan, L. G.; Kasymova, T. D.; Yuldashev, P. Kh.

doi:10.1023/a:1025466030937

Cited by 15 publications

(4 citation statements)

References 10 publications

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“…Its consumption has been attributed to aid digestion. Previous researchers focused their study on papain activity from the latex of the unripe fruit or other parts of the plant, and also in the quantification of papain present in the pulp [3,4]. Packaging and handling systems have been developed in many countries to move products from farm to consumer expeditiously in order to minimize quality degradation.…”

Section: Introductionmentioning

confidence: 99%

Standardisation for Preparation and Storage Stability of Papaya (Carica papaya) Candy

Ashish,

Shrivastava,

Prasad

et al. 2023

IJECC

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The lab experiment was conducted in the year 2021-2022 at Post Harvest Lab, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, in order to Standardisation for preparation and Storage Stability of Papaya Candy (Carica papaya) to evaluate the physico- chemical properties and consumers acceptability of different treatments Quality Characters. From the above experiment T7 (1Kg papaya slice + sugar syrup @75o B + Ginger extract@5%) showed better results in combination. Total soluble solids (T.S.S.), Total titratable acidity, Ascorbic acid content, Reducing sugar, non-reducing sugar, Total sugar, sensory evaluation of candy, Colour, Texture, Flavour, Taste, Overall acceptability whereas minimum was observed in T0 (1Kg papaya slice + sugar syrup @75o B +Control).

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Section: Introductionmentioning

confidence: 99%

Standardisation for Preparation and Storage Stability of Papaya (Carica papaya) Candy

Ashish,

Shrivastava,

Prasad

et al. 2023

IJECC

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“…According to Mezhlumyan et al (2003), the strong proteolytic action of exogenous proteases could increase the digestibility of protein sources. Another benefit would be the possible release of amino acids, which could provide flavor and taste to food through the neuroreceptors of dogs (NRC, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of the addition of papain enzyme on digestive parameters and palatability of extruded diets for dogs

Santos

Sá

Souza

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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Common protein sources used in the manufacturing of diets for dogs are derived from by-products, which may have reduced digestibility depending on the source. This study evaluated the effect of the addition of a protease, the papain enzyme, as a supplement to extruded diets on palatability, nutrient digestibility, and fecal production and quality of dogs. A diet was formulated with poultry by-product meal, meat and bone meal, and feather meal as protein sources. This formula was divided into three isonutrient diets: one negative control (NC), without enzymes; treatment one (EZ1) with addition of 855.000 UI of papain per kilogram of diet, and treatment two (EZ2) with addition of 2.280.000 UI of papain per kilogram of diet, both added before extrusion. The experiment followed a randomized block design, with two blocks of nine animals (three animals per treatment in each block), 18 dogs in total, and six replicates per treatment. Data were submitted to analysis of variance and the means of three treatments were compared by polynomial contrasts (P < 0.05). No differences in the coefficients of total tract apparent digestibility of nutrients nor changes in palatability, pH, and fecal production among treatments were found with the addition of different doses of enzyme to the diets (P > 0.05). The fecal score was reduced with increased addition of enzyme (P < 0.05). Keywords: Additive. Canine. Digestibility. Protein. ResumoAs fontes comuns de proteína utilizadas na fabricação de rações para cães são oriundas de coprodutos, os quais podem apresentar digestibilidade reduzida de acordo com a fonte. Este estudo avaliou os efeitos da adição da enzima papaína em dietas secas e extrusadas na palatabilidade, digestibilidade dos nutrientes, qualidade e produção fecal de cães adultos. Uma dieta foi formulada contendo farinha de vísceras de frango, farinha de carne e ossos e farinha de penas hidrolisadas como fontes proteicas. Esta foi posteriormente dividida em três dietas isonutrientes: controle negativo (CN) sem adição da enzima; adição de 855.000 UI de papaína por quilograma de ração (EZ1); e adição de 2.280.000 UI de papaína por quiilograma de ração (EZ2), ambas adições feitas antes da extrusão. O experimento seguiu delineamento em blocos casualizados, com dois blocos de nove animais (três animais por tratamento em cada bloco), totalizando 18 cães, e seis repetições por tratamento. Os dados obtidos foram submetidos a análise de variância, com as médias dos três tratamentos comparadas por contrastes polinomiais (P < 0,05). Não foram verificadas diferenças nos coeficientes de digestibilidade aparente dos nutrientes ou mesmo alterações na palatabilidade, pH e produção de fezes entre os tratamentos com diferentes inclusões de enzima (P > 0,05). Apenas o escore fecal reduziu com o aumento da adição da enzima (P < 0,05). Palavras-chave: Aditivo. Canino. Digestibilidade. Proteína.

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“…We previously found a linear dependence between the concentration of proteinase inhibitors and the wilt resistance. As a result, genetically fortified cotton varieties with a high content of proteinase inhibitor-proteins could be sought [3].Varieties of the starting selection material with a high protein content and many other agriculturally favorable factors that are very valuable for further selection can be produced by crossing cultured varieties with wild species. By evaluating hybrid families (lines) based on their inhibitory data, we identified selection seed material.…”

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confidence: 99%

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confidence: 99%

Inhibitory and Immunochemical Analysis of Cotton Hybrids

Mezhlumyan

Yuldashev

Kim³

et al. 2005

Chem Nat Compd

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The discovery in selection studies of a positive correlation between the content of proteinase inhibitors and the resistance of plants to diseases and pests made it possible to use a new approach in which markers of plant resistance can act as proteinase inhibitors [1,2]. We previously found a linear dependence between the concentration of proteinase inhibitors and the wilt resistance. As a result, genetically fortified cotton varieties with a high content of proteinase inhibitor-proteins could be sought [3].Varieties of the starting selection material with a high protein content and many other agriculturally favorable factors that are very valuable for further selection can be produced by crossing cultured varieties with wild species. By evaluating hybrid families (lines) based on their inhibitory data, we identified selection seed material. This enabled lines with a high content of proteinase inhibitors to be selected. The activities of these in the studied cotton lines were determined using the Izotova-Stepanov method [4]. Table 1 presents the results of the inhibitory analysis.It can be seen that proteinase inhibitors isolated from seeds of cotton lines L-408, L-842, L-1708, L-218, and L-215 have the greatest activity. Of the studied regional varieties, Omad had the highest content of proetinase inhibitors. Results of resistance analyses in our laboratories and under field conditions have shown that the lines and Omad variety listed above have not only high wilt resistance but also resistance to black root rot and gummosis. Furthermore, it has been found under field conditions that the Omad variety and lines L-408, L-1708, L-842, L-215, and L-162 are valuable for selection because of their agricultural characteristics (early ripening, production, amount and length of fiber, size of bolls, mass of 1000 seeds, oil content).Immunochemical analysis was used to determine the immumological relationship of the studied hybrid combinations (lines) and controls [5]. Proteinase inhibitors isolated from cotton seeds of controls Namangan-77, Omad, C-6524, and C-6530 were used as the antigen to immunize rabbits by the scheme developed by us of intramuscular and subcutaneous injection at 10 mg/kg mass. The titer of each serum was found by the Ouchterloni technique in agar (1%) and was 1/64. It should be noted that varieties C-6524, C-6530, Namangan-77, and Omad were used as controls because they are direct or indirect parents of the studied lines. The sera obtained were used for screening the studied lines by the Feinberg method of gradient concentration of antibodies (the greatest culture of the antigen for which a precipitation reaction is still observed) [6]. Titration of proteinase inhibitors isolated from seeds of the studied cotton lines showed the different degree of immunochemical similarity with the controls. Line L-408 was most similar to variety C-6524. Lines L-842, L-178, L-218, L-162, and L-408 showed a relatively close similarity to the Omad and C-6530 varieties.Thus, the experiments found that lines with a hi...

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Cited by 15 publications

References 10 publications

Standardisation for Preparation and Storage Stability of Papaya (Carica papaya) Candy

Standardisation for Preparation and Storage Stability of Papaya (Carica papaya) Candy

Effect of the addition of papain enzyme on digestive parameters and palatability of extruded diets for dogs

Inhibitory and Immunochemical Analysis of Cotton Hybrids

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