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Wiltshire, Karen Helen; Boersma, Maarten; Möller, A. B.; Buhtz, H.

doi:10.1023/a:1009911418606

Cited by 138 publications

(24 citation statements)

References 26 publications

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“…The primary metabolites are source of bioactive metabolites, such as vitamins and enzymes, which are commercially beneficial due to their antioxidant, anti-inflammatory, antiangiogenic, antiobesity, and anticancer properties [3]. Commonly used extraction technologies via chemical and mechanical methods include expellers, liquid-liquid extraction (solvent extraction), super-critical fluid extraction (SFE), and ultrasound techniques [4–7]. …”

Section: Introductionmentioning

confidence: 99%

“…The collapse of the bubbles emits shockwaves which shatter the cell wall to enable the release of intracellular materials. Wiltshire et al [7] reported ultrasound extraction yields of 90% for fatty acids and pigments from Scenedesmus obliquus . Although ultrasound extraction of lipids from microalgae is quite effective at small scale, numerous technoeconomic challenges arise with large scale applications.…”

Section: Introductionmentioning

confidence: 99%

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Subcritical Water Technology for Enhanced Extraction of Biochemical Compounds fromChlorella vulgaris

Awaluddin

Thiruvenkadam

Izhar

et al. 2016

BioMed Research International

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Subcritical water extraction (SWE) technology has been used for the extraction of active compounds from different biomass materials with low process cost, mild operating conditions, short process times, and environmental sustainability. With the limited application of the technology to microalgal biomass, this work investigates parametrically the potential of subcritical water for high-yield extraction of biochemicals such as carbohydrates and proteins from microalgal biomass. The SWE process was optimized using central composite design (CCD) under varying process conditions of temperature (180–374°C), extraction time (1–20 min), biomass particulate size (38–250 μm), and microalgal biomass loading (5–40 wt.%). Chlorella vulgaris used in this study shows high volatile matter (83.5 wt.%) and carbon content (47.11 wt.%), giving advantage as a feedstock for biofuel production. The results showed maximum total carbohydrate content and protein yields of 14.2 g/100 g and 31.2 g/100 g, respectively, achieved under the process conditions of 277°C, 5% of microalgal biomass loading, and 5 min extraction time. Statistical analysis revealed that, of all the parameters investigated, temperature is the most critical during SWE of microalgal biomass for protein and carbohydrate production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Subcritical Water Technology for Enhanced Extraction of Biochemical Compounds fromChlorella vulgaris

Awaluddin

Thiruvenkadam

Izhar

et al. 2016

BioMed Research International

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“…Both primary and secondary metabolites from microalgae are considered promising natural sources of bioactive compounds since approx. 40% of global photosynthesis being due to these microorganisms [4]. Figure 1 presents a simplified scheme of the primary and secondary metabolism of microalgae.…”

Section: Bioactive Metabolites Found In Microalgae Speciesmentioning

confidence: 99%

Screening of Bioactive Compounds Synthesized by Microalgae: a Progress Overview on Extraction and Chemical Analysis

Patras¹,

Moraru²,

Socaciu³

2018

Studia UBB Chemia

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ABSTRACT. Considering the high biodiversity of microalgae and their important impact on economy and ecological balance, new screening techniques are needed for their phenotypic characterization and efficient valorization of bioactive components. This represents one the key purpose of the emerging bioeconomy concept, involving new biorefinery technologies coupled with advanced analytical tools. The article presents an overview of the recent extraction and chemical techniques used to recover, separate and characterize the main bioactive molecules (lipids and fatty acids, carbohydrates, proteins and mycosporine-like amino acids chlorophylls and carotenoid pigments) syntesized by microalgae, considering their interesting applications in food, pharmaceutical and cosmetic industries. Special focuses will be directed towards the cell disintegration and extraction procedures, identification and quantification of main metabolites by advanced analytical techniques.

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“…Apart from the numerous extraction and purification protocols developed to obtain proteins from S. platensis microalgae [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28], several analytical methods have been developed in parallel to characterize these phycobiliproteins. Thus, analytical methods have frequently used UV spectroscopy [13,16,23,29] and/or SDS-PAGE of the denatured proteins [13,23,29,30].…”

Section: Introductionmentioning

confidence: 99%

Characterization of proteins from Spirulina platensis microalga using capillary electrophoresis‐ion trap‐mass spectrometry and capillary electrophoresis‐time of flight‐mass spectrometry

Simó¹,

Herrero²,

Neusüß

et al. 2005

Electrophoresis

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In this work, a new capillary electrophoresis-mass spectrometry (CE-MS) procedure is developed to analyze proteins in Spirulina platensis microalgae. It is demonstrated that a fine optimization of several separation parameters is essential in order to achieve suitable CE-MS analysis of these proteins in natural extracts from microalgae. Namely, optimization of the composition of the separation buffer, electrospray conditions, and washing routine between runs are required in order to obtain reliable and reproducible CE-MS analyses of the main proteins found in this microalga (namely, allophycocyanin-alpha chain, allophycocyanin-beta, c-phycocyanin-alpha, and c-phycocyanin-beta). The relative molecular mass of these biopolymers is determined using two different MS instruments coupled to CE, i.e., CE-ion trap-MS and CE-time of flight-MS (CE-TOF-MS). A comparison between the results obtained with both instruments is carried out. The high resolution of the TOF-MS enables the distinction of small modifications in proteins and, thus, a more accurate mass determination. Interestingly, molecular mass values obtained by both CE-MS procedures agree very well while these experimental values are only in partial agreement with those theoretically expected (i.e., genetically derived masses). Some protein modifications due to amino acids exchange induced by nucleotide codon mutations are proposed to explain this difference.

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Cited by 138 publications

References 26 publications

Subcritical Water Technology for Enhanced Extraction of Biochemical Compounds fromChlorella vulgaris

Subcritical Water Technology for Enhanced Extraction of Biochemical Compounds fromChlorella vulgaris

Screening of Bioactive Compounds Synthesized by Microalgae: a Progress Overview on Extraction and Chemical Analysis

Characterization of proteins from Spirulina platensis microalga using capillary electrophoresis‐ion trap‐mass spectrometry and capillary electrophoresis‐time of flight‐mass spectrometry

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