YNCRG Inhibited Metabolic Syndrome Through Appetite Suppression and Improved Lipid Metabolism in Metabolic Syndrome Model Rats

Kudo, Maya; Hayashi, Misa; Tian, Peng; Liu, Danyang; Wu, Lili; Li, Wei; Hong, Zhaoyang; Zhao, Yitang; Nishigaki, Toshiaki; Nishihara, Masaaki; Koike, Kazuo; Liu, Tonghua; Gao, Ming

doi:10.21926/obm.icm.2003033

Cited by 4 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…YNCRG effectively reduced serum TG, CHO, AST, and ALT levels after eight weeks of intervention, indicating that YNCRG can improve lipid metabolism and liver function (Figures 1B-E). These results were similar to those of our previous study [8].…”

Section: Effects Of Yncrg On Weight and Metabolic Parameterssupporting

confidence: 93%

“…The WKY group could drink water freely, the control group was provided water every day via gavage, and the YNCRG group was gavaged with an aqueous solution of YNCRG (3.6 g/kg body weight/day). As mentioned previously, we administered multiple doses of YNCRG, and we selected the experimental period for which YNCRG had the strongest effect on the metabolic syndrome of rats [8]. We weighed the rats daily.…”

Section: Animals and Treatmentmentioning

confidence: 99%

“…YNCRG can effectively reduce total cholesterol levels and AST and ALT levels in SHR. In Cg-Lepr cp /NDmcr rats ((SHRcp rat(s)), YNCRG can inhibit lipogenesis and promote lipolysis in the adipose tissue [8]. YNCRG is a herbal formula prepared by our team and consists of five ingredients, including Tochu leaf extract, Red koji rice (Monascus purpureus), Noni (Morinda citrifolia), Lcitrulline, and gamma-aminobutyric acid.…”

Section: Introductionmentioning

confidence: 99%

“…YNCRG is a herbal formula prepared by our team and consists of five ingredients, including Tochu leaf extract, Red koji rice (Monascus purpureus), Noni (Morinda citrifolia), Lcitrulline, and gamma-aminobutyric acid. The pharmacological activities of these five ingredients were discussed in our previous study [8]. The SHRCP rat is a common animal model used for studying metabolic syndrome, and there is a close relationship between metabolic syndrome and NAFLD [9,10].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

YNCRG Alleviated Hepatic Steatosis in Metabolic Syndrome Model Rats by Improving Lipid Metabolism and Chronic Inflammatory Reaction

Sun¹,

Kudo²,

Hayashi³

et al. 2021

OBM ICM

Self Cite

View full text Add to dashboard Cite

YNCRG is a combination of five traditional Chinese ingredients with medicinal properties. We studied its effects and mechanism of action on a rat model of non-alcoholic fatty liver with metabolic syndrome. Eight-week-old male SHRcp rat(s) and Wister Kyoto (WKY) rats were adaptively fed for one week, after which they were randomly divided into three groups, including the control group (n = 9), the YNCRG group (n = 8), and the WKY group (n = 8). The WKY group could freely consume water, the control group was provided water every day, and the YNCRG group was gavaged with an aqueous solution of YNCRG (3.6 g/kg body weight/day) for eight weeks. After eight weeks of intervention, YNCRG effectively reduced the weight of rats and significantly reduced the serum levels of AST, ALT, CHO, and TG compared to the weight of the rats in the control group. The RT-PCR results showed that mRNA levels of NF-κB, TLR4, TNF-α, and IL-1β increased, and the levels of MCP-1 and ICAM-1 also increased, but YNCRG effectively reduced the mRNA levels. The results of the Western blotting assay showed that YNCRG did not affect the AMPK/ACC signaling pathway, but it strongly promoted the phosphorylation of PKA, ERK, and C/EBPβ in rat livers. YNCRG also significantly promoted the phosphorylation of IRS-1 (Tyr465) but inhibited IRS-1 (Ser1101); both are upstream factors of ERK. We found that YNCRG can reduce the accumulation of lipids in the liver of rats with metabolic syndrome, probably by affecting lipid metabolism and the signaling pathway associated with inflammatory reactions.

show abstract

Section: Effects Of Yncrg On Weight and Metabolic Parameterssupporting

confidence: 93%

Section: Animals and Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

YNCRG Alleviated Hepatic Steatosis in Metabolic Syndrome Model Rats by Improving Lipid Metabolism and Chronic Inflammatory Reaction

Sun¹,

Kudo²,

Hayashi³

et al. 2021

OBM ICM

Self Cite

View full text Add to dashboard Cite

show abstract

“…The amplification was performed as follows in a Thermal Cycler Dice (TAKARA BIO INC., Japan): 1 cycle at 95°C for 30 s, and 40 cycles at 95°C for 5 s and 60°C for 30 s. We determined the fold differences in gene expression levels using the 2 −ΔΔCT method. The ratio of mRNA expression levels was normalized to the house keeping gene glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (Kudo et al., 2020 ; Li et al., 2015 ). We show a comparison of gene expression by L‐Cit administration when the gene expression level in the control group is 100%.…”

Section: Methodsmentioning

confidence: 99%

L‐citrulline inhibits body weight gain and hepatic fat accumulation by improving lipid metabolism in a rat nonalcoholic fatty liver disease model

Kudo

Yamagishi²,

Suguro³

et al. 2021

Food Science & Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

Amycenone reduces excess body weight and attenuates hyperlipidaemia by inhibiting lipogenesis and promoting lipolysis and fatty acid β-oxidation in KK-A^yobese diabetic mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

Excess body weight and hyperlipidaemia cause severe health problems and have social implications. Amycenone is an active substance extracted from Yamabushitake mushrooms with no reports of its activity against excess body weight and hyperlipidaemia. This research clarifies the effects and mechanisms of action of amycenone on the inhibition of body weight excess and hyperlipidaemia attenuation using KK-Ay mice. Amycenone or water was administered to 8-week-old male KK-Ay mice by gavage for 8 weeks. Their body weight and food intake were recorded during the experiment. At the end of the experimental period, the mice were dissected, and blood samples, lipid metabolism-related organs and tissues were collected and stored for further analysis. Amycenone treatment suppressed body weight gain and improved serum levels of fasting blood glucose and non-esterified fatty acids. Additionally, serum and hepatic cholesterol and triacylglycerol levels were reduced after this treatment, whereas the phosphorylation levels of AMPK, PKA and HSL increased and the expression level of FAS decreased. The protein level of C/EBPβ and gene expression level of Cpt1 were higher in the perirenal adipose tissue of amycenone-treated KK-Ay mice. Furthermore, amycenone phosphorylated AMPK, PKA and ACC, and PPARγ expression was lower in the mesenteric adipose tissue. The phosphorylation levels of AMPK, LKB1, PKA and ACC were also induced, and FAS expression level was reduced in the liver of the amycenone-treated group. Amycenone could reduce excess body weight and attenuate hyperlipidaemia in KK-Ay mice by inhibiting lipogenesis and promoting lipolysis through lipid metabolism pathway stimulation and fatty acid β-oxidation acceleration.

show abstract

YNCRG Inhibited Metabolic Syndrome Through Appetite Suppression and Improved Lipid Metabolism in Metabolic Syndrome Model Rats

Cited by 4 publications

References 0 publications

YNCRG Alleviated Hepatic Steatosis in Metabolic Syndrome Model Rats by Improving Lipid Metabolism and Chronic Inflammatory Reaction

YNCRG Alleviated Hepatic Steatosis in Metabolic Syndrome Model Rats by Improving Lipid Metabolism and Chronic Inflammatory Reaction

L‐citrulline inhibits body weight gain and hepatic fat accumulation by improving lipid metabolism in a rat nonalcoholic fatty liver disease model

Amycenone reduces excess body weight and attenuates hyperlipidaemia by inhibiting lipogenesis and promoting lipolysis and fatty acid β-oxidation in KK-A^yobese diabetic mice

Contact Info

Product

Resources

About

YNCRG Inhibited Metabolic Syndrome Through Appetite Suppression and Improved Lipid Metabolism in Metabolic Syndrome Model Rats

Cited by 4 publications

References 0 publications

YNCRG Alleviated Hepatic Steatosis in Metabolic Syndrome Model Rats by Improving Lipid Metabolism and Chronic Inflammatory Reaction

YNCRG Alleviated Hepatic Steatosis in Metabolic Syndrome Model Rats by Improving Lipid Metabolism and Chronic Inflammatory Reaction

L‐citrulline inhibits body weight gain and hepatic fat accumulation by improving lipid metabolism in a rat nonalcoholic fatty liver disease model

Amycenone reduces excess body weight and attenuates hyperlipidaemia by inhibiting lipogenesis and promoting lipolysis and fatty acid β-oxidation in KK-Ayobese diabetic mice

Contact Info

Product

Resources

About

Amycenone reduces excess body weight and attenuates hyperlipidaemia by inhibiting lipogenesis and promoting lipolysis and fatty acid β-oxidation in KK-A^yobese diabetic mice