Young and old animals “use” different strategies for adapting to the chronic effects of ethanol

Bozhkov, Anatoly I.; Dobrianska, Nataliia I.; Akzhyhitov, Rustam A.; Novikova, Anna V.; Haiovyi, Stanislav S.; Pankratova, Alona O.; Ivanova, Olena O.; Onipko, Illia R.; Komburlei, Yuliia O.

doi:10.1016/j.tma.2024.01.001

Cited by 2 publications

(1 citation statement)

References 53 publications

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“…Excessive alcohol consumption can cause liver injury [1]. At a concentration of 15-50%, ethanol has chronic effects on test animals, and consumption for 160 days can cause death due to damage to the liver, lymph, and kidneys [2].…”

Section: Introductionmentioning

confidence: 99%

Determination of Ethanol in Vinegar and Beverage by Gas Chromatography: A Validated Method for Halal Verification

Yulirohyami,

Wijaya,

Ruwindya

2024

Ind. J. Chem. Anal.

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Validation of the method for determining ethanol in vinegar and beverages has been carried out in the linearity range of 0.05-1% using GC-FID. This research was carried out using an internal standard solution and without an internal standard solution. The research aims to validate the linearity of ethanol determination at low concentration levels. This validation is useful to ensure that the ethanol test method in the sample can be detected at low concentration levels. In the halal authentication process, the maximum limit for ethanol content is 5%. The method validation results confirm that in the concentration range 0.05-1% it has good linearity with a correlation coefficient (R) without the addition of internal standards and with the addition of standard solutions being 0.9972 (R2=0.9943) and 0.9952 (R2=0.9905). The results of the analysis of variance (ANOVA) test with a 95% confidence interval and 9 degrees of freedom F-count (0.0141) < F-critical (0.3146) show that the range 0.05-1% corresponds to the limit of linearity. The results of the ANOVA test with a 95% confidence interval with df=6 show the F-test value (0.0879) < F-critical (0.2334) indicating that the two proposed methods have high precision and no significant differences. Both methods also have high accuracy and no significant differences in accuracy as shown by the results of the ANOVA test with a 95% confidence interval and degrees of freedom = 6 showing the F-test value (1.5204) < F-critical (4.2839). It is recommended that the results of this validation be useful in the halal verification process for vinegar and beverage products.

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Section: Introductionmentioning

confidence: 99%

Determination of Ethanol in Vinegar and Beverage by Gas Chromatography: A Validated Method for Halal Verification

Yulirohyami,

Wijaya,

Ruwindya

2024

Ind. J. Chem. Anal.

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Copper sulfate and carbon tetrachloride induces a uniform response at the level of the redox system and the nature of this response depends on age

Ivanov,

Lebid-Biletska,

Bozhkov

et al. 2024

Regul. Mech. Biosyst.

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The concept of oxidative stress, which is a development of D. Harman’s idea of random harmful tissue damage by free radicals, remains one of the most popular in the study of pathological processes, including age-dependent chronic changes. The work tested the hypothesis according to which, a shift in equilibrium towards pro-oxidants, i.e. oxidative stress, is the primary adaptive response of the body to exogenous toxic environmental factors. To test this, a number of indicators of the redox system were determined as a response to hepatotoxic compounds of different nature (copper sulfate and carbon tetrachloride) in young and old animals. The amount of lipid hydroperoxides and the activity of a number of antioxidant enzymes were determined in the blood serum of young (3 months) and old (20 months) rats before exposure (initial level) and after repeated sequential injections (3 injections) of copper sulfate at a dose of 1 mg/100 g body weight and carbon tetrachloride in a dose of 0.1 mL in 50% vegetable oil. It was found that in intact (initial level) old animals, the indicators of the redox system are shifted towards antioxidants. After three consecutive administrations of various hepatotoxic compounds, with an interval of 48 hours between administrations, the balance shifted towards pro-oxidants, regardless of the inducer, however, this effect was more pronounced in old animals compared to young ones, relative to their initial level. Such different reactivity of redox system indicators in animals of different ages led to the “evening out” of the initially different redox system indicators. We came to the conclusion that changing the balance in the pro-oxidant-antioxidant system is a universal, primary reaction of the body to endogenous or exogenous factors that perform regulatory functions and, depending on the temporal and functional characteristics of the body, are “transformed” into specific physiological manifestations.

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Young and old animals “use” different strategies for adapting to the chronic effects of ethanol

Cited by 2 publications

References 53 publications

Determination of Ethanol in Vinegar and Beverage by Gas Chromatography: A Validated Method for Halal Verification

Determination of Ethanol in Vinegar and Beverage by Gas Chromatography: A Validated Method for Halal Verification

Copper sulfate and carbon tetrachloride induces a uniform response at the level of the redox system and the nature of this response depends on age

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