Cultural mistrust is a protective mechanism employed by minority group members when risk of victimization from majority group members is high. In Northern Ireland, depending on the context, both Catholics and Protestants may perceive themselves at risk of discrimination, and use similar protective strategies. This study examined the appropriateness of the Cultural Mistrust Inventory in assessing mistrust among members of different religious groups within Northern Ireland. Exploratory and confirmatory factor analyses resulted in an 11‐item, 3‐factor measure of religious mistrust. Mistrust appears to be experienced by both Catholics and Protestants, regardless of group status; with males reporting significantly higher levels of mistrust of the “other” group. Higher levels of mistrust were associated with higher psychological distress.