Yu-Ping-Feng Formula Ameliorates Alveolar-Capillary Barrier Injury Induced by Exhausted-Exercise via Regulation of Cytoskeleton

Abstract

Background: Yu-ping-feng powder (YPF) is a compound traditional Chinese medicine extensively used in China for respiratory diseases. However, the role of YPF in alveolar-capillary barrier dysfunction remains unknown. This study aimed to explore the effect and potential mechanism of YPF on alveolar-capillary barrier injury induced by exhausted exercise.Methods: Male Sprague–Dawley rats were used to establish an exhausted-exercise model by using a motorized rodent treadmill. YPF at doses of 2.18 g/kg was administrated by gavage before exercise training for 10 consecutive days. Food intake-weight/body weight, blood gas analysis, lung water percent content, BALF protein concentration, morphological observation, quantitative proteomics, real-time PCR, and Western blot were performed. A rat pulmonary microvascular endothelial cell line (PMVEC) subjected to hypoxia was applied for assessing the related mechanism.Results: YPF attenuated the decrease of food intake weight/body weight, improved lung swelling and hemorrhage, alleviated the increase of lung water percent content and BALF protein concentration, and inhibited the impairment of lung morphology. In addition, YPF increased the expression of claudin 3, claudin 18, occludin, VE-cadherin, and β-catenin, attenuated the epithelial and endothelial hyperpermeability in vivo and/or in vitro, and the stress fiber formation in PMVECs after hypoxia. Quantitative proteomics discovered that the effect of YPF implicated the Siah2-ubiquitin-proteasomal pathway, Gng12-PAK1-MLCK, and RhoA/ROCK, which was further confirmed by Western blot. Data are available via ProteomeXchange with identifier PXD032737.Conclusion: YPF ameliorated alveolar-capillary barrier injury induced by exhausted exercise, which is accounted for at least partly by the regulation of cytoskeleton.

An updated meta-analysis of Chinese herbal medicine for the prevention of COVID-19 based on Western-Eastern medicine

“…Within immunocompromised and high-risk older populations, there was a trend towards an increase in the CD4 + /CD8 + ratio, implying that CHM might play a role in enhancing patients’ cellular immunity. Moreover, our analysis unveiled that the preventative effect of CHM against COVID-19 was more pronounced in children and older adults ( Fang et al, 2020 ; Wang et al, 2022 ). We believe that the following factors contribute to this observation.…”

“…Seven studies had scores lower than four on the modified Jadad scale, indicating poor overall quality of research. The average NOS score for observational studies was 7.4 ( Fang et al, 2020 ; Lv et al, 2020 ; Wang et al, 2020 ; Zheng and Lu, 2021 ; Wang et al, 2022 ), suggesting the high quality of the included studies. The summary of the included studies in the analysis were shown in Table 4 and Table 5 .…”

“…The incidence of COVID-19 was investigated in eight clinical trials involving 46,165 patients ( Wang et al, 2022 ; Wang F et al, 2021 ; Wang YL et al, 2021 ; Wang ZZ et al, 2021 ; Yan et al, 2021 ; Zhang, 2021 ; Xie et al, 2023 ; Liu et al, 2023 ). Compared to the non-treatment or conventional control group, the use of oral CHM resulted in a substantially reduced incidence of COVID-19 onset (RR = 0.24, 95% CI = 0.11–0.53, p = 0.0004).…”

HDAC3 deficiency protects against acute lung injury by maintaining epithelial barrier integrity through preserving mitochondrial quality control

“…Epithelial barrier integrity is closely associated with the pulmonary function as well as the severity of ALI [ 33 , 34 ]. We next observed whether HDAC3 knockout in AT2 affect alveolar epithelial barrier integrity in mice with ALI.…”

Adjuvant treatment with yupingfeng granules for recurrent respiratory tract infections in children: A systematic review and meta-analysis

“…Ma et al ( 18 ) found that through conventional treatment, YPFG significantly relieved clinical symptoms, prevented acute exacerbations, and improved symptom scores in COPD patients with good safety, which may be related to the anti-fatigue and anti-hypoxia functions of astragalus ( 49 ). Animal experiments show that Yupingfeng (YPF) can improve alveolar-capillary barrier damage induced by exhaustive exercise in rats by modulating the cytoskeleton ( 50 ).…”