We study implications of the four-fermion semileptonic operators at the low-energy and at electroweak (EW) scale in the framework of Standard Model Effective Field Theory (SMEFT). We show how the renormalization group (RG) running effects can play a key role in probing the generic flavour structure of such operators. It is shown that at the 1loop level, through RG running, depending upon the flavour structure these operators can give rise to sizable effects at low energy in the electroweak precision (EWP) observables, the leptonic, quark, as well as the Z boson flavour violating decays. To this end, we isolate the phenomenologically relevant terms in the already known full anomalous dimension matrices (ADMs) and discuss the impact of the QED+QCD running in the Weak effective field theory (WET) and the SMEFT running due to gauge and Yukawa interactions on the dim-4 and dim-6 operators at the low energy. Considering all the relevant processes, we derive lower bounds on new physics (NP) scale Λ for each semileptonic operator, keeping a generic flavor structure. In addition, we also report the allowed ranges for the Wilson coefficients at a fixed value of Λ = 3 TeV.