Z-scan method to measure the nonlinear optical behavior of cells for evaluating the cytotoxic effects of chemotherapy and hyperthermia treatments

Minaei, Soraya Emamgholizadeh; Ghader, Alireza; Ardakani, Ali Abbasian; Khoei, Samideh; Asgari, Hamid; Ara, M.H. Majles

doi:10.1007/s10103-020-03148-2

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…Indeed, the efficiency of novel treatments should be evaluated in vitro before utilizing in vivo. In this regard, another study by Minaei et al 58 was conducted to present the Z-scan technique as an accurate, in vitro procedure to investigate the cytotoxicity of different treatments. In this way, OLN-93 and C6 cells were treated with radiofrequency hyperthermia (HT), Temozolomide (TMZ), and chemo-hyperthermia (HT + TMZ).…”

Section: Discussionmentioning

confidence: 99%

“…The results of Z-scan method were in accordance with the data of clonogenic assay. 58 Moreover, in another study, researchers demonstrated that the Z-scan technique can be considered as a reliable method by the potential to detect cell changes throughout treatments with HT. 59 In addition, a previous report confirmed the advantage of chemo-photothermal (PTT) effect to understand the release of DOX from hairpin DNA.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Radiotherapy in Combination With Irinotecan and 17-AAG on Bcl-2 and Caspase 3 Gene Expression in Colorectal Cancer Cells

et al. 2022

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Introduction: In this study, the cytotoxic and anti-cancer effects of Irinotecan as a conventional chemotherapeutic agent compared to 17-(allyl amino)-17-demethoxygeldanamycin (17-AAG) as possible radiosensitizers in the HCT-116 cell line were investigated. Methods: HCT-116 cells were treated with various concentrations of irinotecan and 17-AAG and also irradiated with a 2-Gy of X-ray radiation. Then, the cell viability was examined by a water-soluble tetrazolium-1 assay after 24 hours. For single therapies and double and triple combination cases, IC50, 0.5×IC50 and 0.25×IC50 concentrations of each drug were selected respectively for a terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay and other tests. In treated and untreated cells, the caspase 3 and Bcl-2 gene expression ratios were evaluated by the real-time PCR method. Likewise, caspase 3 activity was detected with a colorimetric assay. Results: In all combined treatments, including 17-AAG- radiation, irinotecan - radiation, irinotecan -17-AAG, and irinotecan-17-AAG-radiation, decreased cellular viability and increased TUNEL positive cells were presented versus the control group (P<0.05). There were increased TUNEL positive cells in the triple combination, in concentrations of 0.25×IC50 of each drug, in comparison with single and double agent treatments. Moreover, in triple combination, the caspase 3 mRNA level and caspase 3 activity increased versus related single treatments. Likewise, in the irinotecan-17-AAG-radiation combined treatment and the 17-AAG-radiation double treatment, the Bcl-2 gene expression level decreased in comparison with single therapies. Conclusion: It can be indicated that the combination of chemo-radiotherapy versus single treatments has significant anti-cancer effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Radiotherapy in Combination With Irinotecan and 17-AAG on Bcl-2 and Caspase 3 Gene Expression in Colorectal Cancer Cells

et al. 2022

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“…Bax 5 0 -CGATGAACTGGACAACAACATGG-3 0 5 0 -GCAAAGTAGAAAAGGGCAACCAC-3 0 Bcl-2 5 0 -TGGATGACTGAGTACCTGAA-3 0 5 0 -GAGACAGCCAGGAGAAATCAAA-3 0 GAPDH 5 0 -AGTTCAACGGCACAGTCAAG-3 0 5 0 -TACTCAGCACCAGCATCACC-3 0 heightened uptake of DOX/MJNPs-FLA and MJNPs-FLA by the C6 cells, which possess elevated levels of folate receptor expression on their surfaces. 30 The results also showed that MJNPs-FLA are highly biocompatible and suppress the growth of the C6 glioma and OLN-93 cell lines in a concentration-dependent manner. In vivo and ex vivo experiments Liver enzyme tests.…”

Section: Target Gene Forward Primer Reverse Primermentioning

confidence: 81%

“…The findings substantiate that the C6 cell line exhibited a greater uptake of MJNPs-FLA compared to MJNPs-FL, attributed to the high expression levels of the folate receptor on the surfaces of C6 cells. 30 From the Prussian blue results, the conversion of ferric to ferrous iron resulted in the development of blue precipitates, 31 which served as a sign of the presence of nanoparticles inside the C6 cells (see the arrows in Fig. 5a).…”

Section: Resultsmentioning

confidence: 99%

Active targeted delivery of theranostic thermo/pH dual-responsive magnetic Janus nanoparticles functionalized with folic acid/fluorescein ligands for enhanced DOX combination therapy of rat glioblastoma

Ali,

Khoee,

Mafakheri

et al. 2024

J. Mater. Chem. B

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“…In 2021, Minaei SE et al conducted a study demonstrating the efficacy of the Z-scan technique in assessing the toxic effects of chemotherapy and hyperthermia therapies. This was achieved by quantifying the nonlinear optical response of cellular entities [37].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Z-scan technique for quantifying circulating cell-free DNA (ccfDNA) extracted from blood plasma as a potential biomarker for various cancers

Ara,

Tarek,

Biswas

et al. 2024

Biomed. Phys. Eng. Express

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AbstractThe Z-scan technique is a nonlinear optical method that has found applications in characterizing various materials, particularly those exhibiting nonlinear optical response (NLOR). This study applies the continuous wave (CW) Z-scan technique to examine the NLOR in terms of the nonlinear optical phase shifts (ΔΦ_0) exhibited by the ccfDNA extracted from blood plasma samples collected from a group constituting 30 cancer-diagnosed patients and another group constituting 30 non-diagnosed individuals. The cancer group exhibited significantly higher ΔΦ_0vs. incident power slopes compared to the non-cancer group (0.34 vs 0.12) providing a clear distinction between the two groups. The receiver operating characteristic (ROC) curve analysis of the results indicates a clear separation between cancer and non-cancer groups, along with a 94% accuracy rate of the data. The Z-scan results are corroborated by spectrophotometric analysis, revealing a consistent trend in the concentration values of ccfDNA samples extracted from both cancerous and non-cancerous samples, measuring 3.24 and 1.41 respectively. Additionally, more sensitive fluorometric analyses of the respective samples demonstrate significantly higher concentrations of ccfDNA in the cancer group, further affirming the correlation with the Z-scan results. The study suggests that the Z-scan technique holds promise as an effective method for cancer detection, potentially contributing to improved oncology diagnosis and prognosis in the future.

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Z-scan method to measure the nonlinear optical behavior of cells for evaluating the cytotoxic effects of chemotherapy and hyperthermia treatments

Cited by 5 publications

References 38 publications

Effects of Radiotherapy in Combination With Irinotecan and 17-AAG on Bcl-2 and Caspase 3 Gene Expression in Colorectal Cancer Cells

Effects of Radiotherapy in Combination With Irinotecan and 17-AAG on Bcl-2 and Caspase 3 Gene Expression in Colorectal Cancer Cells

Active targeted delivery of theranostic thermo/pH dual-responsive magnetic Janus nanoparticles functionalized with folic acid/fluorescein ligands for enhanced DOX combination therapy of rat glioblastoma

Investigating the Z-scan technique for quantifying circulating cell-free DNA (ccfDNA) extracted from blood plasma as a potential biomarker for various cancers

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