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Carr, K. E.; Hazzard, R A; Reid, Shane W.; Hodges, Gisèle M.

doi:10.1023/a:1016064320334

Cited by 76 publications

(15 citation statements)

References 8 publications

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“…LSs are approximately 25 μm in size. Previous studies investigating oral transport of particles have determined that particles approximately 2 μm or less are appropriately-sized for uptake via M-cells of the Peyer's patches [44]. However, the same study also reported uptake of 20 μm diameter particles, although with reduced frequency.…”

Section: Resultsmentioning

confidence: 98%

Pollen grains for oral vaccination

Atwe¹,

Ma²,

Gill³

2014

Journal of Controlled Release

147

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Oral vaccination can offer a painless and convenient method of vaccination. Furthermore, in addition to systemic immunity it has potential to stimulate mucosal immunity through antigen-processing by the gut-associated lymphoid tissues. In this study we propose the concept that pollen grains can be engineered for use as a simple modular system for oral vaccination. We demonstrate feasibility of this concept by using spores of Lycopodium clavatum (clubmoss) (LSs). We show that LSs can be chemically cleaned to remove native proteins to create intact clean hollow LS shells. Empty pollen shells were successfully filled with molecules of different sizes demonstrating their potential to be broadly applicable as a vaccination system. Using ovalbumin (OVA) as a model antigen, LSs formulated with OVA were orally fed to mice. LSs stimulated significantly higher anti-OVA serum IgG and fecal IgA antibodies compared to those induced by use of cholera toxin as a positive-control adjuvant. The antibody response was not affected by pre-neutralization of the stomach acid, and persisted for up to seven months. Confocal microscopy revealed that LSs can translocate in to mouse intestinal wall. Overall, this study lays the foundation of using LSs as a novel approach for oral vaccination.

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Section: Resultsmentioning

confidence: 98%

Pollen grains for oral vaccination

Atwe¹,

Ma²,

Gill³

2014

Journal of Controlled Release

147

View full text Add to dashboard Cite

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“…On the other hand, whole-body inhalation exposure to ultrafine carbon particles have suggested some deposition in the liver, which has been speculated to originate from gastro intestinal exposure and uptake from the gut (Oberdörster et al 2002). In addition, the uptake of C 60 fullerenes and polystyrene latex microspheres from the gastrointestinal tract into blood circulation has been estimated in the range of 1% of the applied dose (Carr et al 1996; Yamago et al 1995). However, it is interesting to note that in drug delivery research, there is acceptance that particulate matter can be absorbed from the gastrointestinal tract, and this feature of nanomaterials is being used as an approach of altering the pharmacokinetic behavior of drugs (Florence 2004).…”

Section: Discussionmentioning

confidence: 99%

Oxidatively Damaged DNA in Rats Exposed by Oral Gavage to C ₆₀ Fullerenes and Single-Walled Carbon Nanotubes

Folkmann

Risom

Jacobsen

et al. 2009

Environ Health Perspect

224

117

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BackgroundC60 fullerenes and single-walled carbon nanotubes (SWCNT) are projected to be used in medicine and consumer products with potential human exposure. The hazardous effects of these particles are expected to involve oxidative stress with generation of oxidatively damaged DNA that might be the initiating event in the development of cancer.ObjectiveIn this study we investigated the effect of a single oral administration of C60 fullerenes and SWCNT.MethodsWe measured the level of oxidative damage to DNA as the premutagenic 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) in the colon mucosa, liver, and lung of rats after intragastric administration of pristine C60 fullerenes or SWCNT (0.064 or 0.64 mg/kg body weight) suspended in saline solution or corn oil. We investigated the regulation of DNA repair systems toward 8-oxodG in liver and lung tissue.ResultsBoth doses of SWCNT increased the levels of 8-oxodG in liver and lung. Administration of C60 fullerenes increased the hepatic level of 8-oxodG, whereas only the high dose generated 8-oxodG in the lung. We detected no effects on 8-oxodG in colon mucosa. Suspension of particles in saline solution or corn oil yielded a similar extent of genotoxicity, whereas corn oil per se generated more genotoxicity than the particles. Although there was increased mRNA expression of 8-oxoguanine DNA glycosylase in the liver of C60 fullerene-treated rats, we found no significant increase in repair activity.ConclusionsOral exposure to low doses of C60 fullerenes and SWCNT is associated with elevated levels of 8-oxodG in the liver and lung, which is likely to be caused by a direct genotoxic ability rather than an inhibition of the DNA repair system.

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“…Particles (1–2 µm) have been shown to be effective in inducing an immune response because the particles can be selectively taken up by the M cells and can be actively transported across the epithelium [93]. They can also selectively target either M cells or other intestinal cells such as enterocytes by use of targeting moieties or by incorporating mucoadhesive polymers [55].…”

Section: Mucosal Vaccine Delivery Systemsmentioning

confidence: 99%

Mucosal vaccine delivery: Current state and a pediatric perspective

Shakya

Chowdhury

Tao

et al. 2016

Journal of Controlled Release

127

115

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Most childhood infections occur via the mucosal surfaces, however, parenterally delivered vaccines are unable to induce protective immunity at these surfaces. In contrast, delivery of vaccines via the mucosal routes can allow antigens to interact with the mucosa-associated lymphoid tissue (MALT) to induce both mucosal and systemic immunity. The induced mucosal immunity can neutralize the pathogen on the mucosal surface before it can cause infection. In addition to reinforcing the defense at mucosal surfaces, mucosal vaccination is also expected to be needle-free, which can eliminate pain and the fear of vaccination. Thus, mucosal vaccination is highly appealing, especially for the pediatric population. However, vaccine delivery across mucosal surfaces is challenging because of the different barriers that naturally exist at the various mucosal surfaces to keep the pathogens out. There have been significant developments in delivery systems for mucosal vaccination. In this review we provide an introduction to the MALT, highlight barriers to vaccine delivery at different mucosal surfaces, discuss different approaches that have been investigated for vaccine delivery across mucosal surfaces, and concludes with an assessment of perspectives for mucosal vaccination in the context of the pediatric population.

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Cited by 76 publications

References 8 publications

Pollen grains for oral vaccination

Pollen grains for oral vaccination

Oxidatively Damaged DNA in Rats Exposed by Oral Gavage to C ₆₀ Fullerenes and Single-Walled Carbon Nanotubes

Mucosal vaccine delivery: Current state and a pediatric perspective

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Untitled

Cited by 76 publications

References 8 publications

Pollen grains for oral vaccination

Pollen grains for oral vaccination

Oxidatively Damaged DNA in Rats Exposed by Oral Gavage to C 60 Fullerenes and Single-Walled Carbon Nanotubes

Mucosal vaccine delivery: Current state and a pediatric perspective

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Product

Resources

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Oxidatively Damaged DNA in Rats Exposed by Oral Gavage to C ₆₀ Fullerenes and Single-Walled Carbon Nanotubes