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An ever increasing wealth of ancient biological material is providing opportunities to study biomolecules. Animal, plant, and microbial samples dating back hundreds, thousands, and even millions of years have been preserved in a dry state under climatic conditions ranging from the arctic to hot deserts. Various small molecules, often crystalized or polymerized, have improved preservation. Modern methods such as polymerase chain reaction (PCR), mass spectrometry, and shotgun sequencing have detected and characterized ancient biomolecules. Modern sequencing has the capacity not only to assemble the whole genome of the target host but also those of the host's parasites, mutualists, and commensals. The study of ancient RNA has barely begun. Several studies show that RNA has been preserved for decades to hundreds of years and the germination of ancient seeds implies that messenger RNA can be preserved for thousands of years. This review briefly examines the types of ancient materials available and assesses their suitability for the study of ancient RNA. Sequencing RNA from this material has the potential not only to illuminate the target host's transcriptome and small RNAs but also to characterize the host's RNA parasites: viruses and viroids.
An ever increasing wealth of ancient biological material is providing opportunities to study biomolecules. Animal, plant, and microbial samples dating back hundreds, thousands, and even millions of years have been preserved in a dry state under climatic conditions ranging from the arctic to hot deserts. Various small molecules, often crystalized or polymerized, have improved preservation. Modern methods such as polymerase chain reaction (PCR), mass spectrometry, and shotgun sequencing have detected and characterized ancient biomolecules. Modern sequencing has the capacity not only to assemble the whole genome of the target host but also those of the host's parasites, mutualists, and commensals. The study of ancient RNA has barely begun. Several studies show that RNA has been preserved for decades to hundreds of years and the germination of ancient seeds implies that messenger RNA can be preserved for thousands of years. This review briefly examines the types of ancient materials available and assesses their suitability for the study of ancient RNA. Sequencing RNA from this material has the potential not only to illuminate the target host's transcriptome and small RNAs but also to characterize the host's RNA parasites: viruses and viroids.
Numerous and various ecological and morphological groups of viable micro-organisms survive under permafrost conditions. They are the only known living organisms preserved over a geologically significant time. Their preservation within permafrost is of interest from both biological and geocryological points of view and forms the basis for a number of investigations in different scientific fields. The latter are performed not only upon morphological but also upon functional characteristics of viable cells. This approach is relevant to questions concerning the protective properties of unfrozen water and may provide new ways of solving major problems of historical geocryology. Of special interest is the interaction of both biological and geological knowledge to better understand the spatial and temporal limits of the biosphere. RESUMEDe nombreux groupes varies de micro-organismes survivent a leur emprisonnement dans le pergelisol. 11s constituent les seuls organismes qui sont prkservks aprks un temps gkologique significatif. Leur prkservation au sein du pergklisol est intkressante a la fois au point de vue biologique et gkocryologique, et elle constitue l'objet de nombreuses recherches dans diffkrents domaines scientifiques, notamment en ce qui concerne la morphologie mais aussi en ce qui concerne les caractkristiques fonctionnelles des cellules rksistantes. Ces recherches peuvent apporter des donnkes propos des possibilitks protectives de l'eau non gelee et peuvent aussi fournir des solutions a des problitmes fondamentaux de geocryologie historique. Ces recherches concernent en outre l'interaction entre les connaissances biologiques et gkologiques et aideront ZI mieux comprendre les limites spatiales et temporelles de la biosphitre. KEY WORDS: permafrost; micro-organisms: biology COLD-ADAPTED MICRO-ORGANISMSorganisms in natural environments, which mostly are at low temperatures. Including seawater, of Temperature is one of the most important which two-thirds has a temperature of around parameters regulating the activities of micro-+ 2"C, and polar regions, more than 80% of the
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