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Spahn, Donat R.

doi:10.1186/cc364

Cited by 169 publications

(75 citation statements)

References 24 publications

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“…45 Moreover, using 19 F-MRI is an attractive technique as it provides positive contrast, and the high-resolution anatomical 1 H clinical images can be registered and superimposed with the highly sensitive 19 F hotspot images to create a precise mapping of where the 19 F-labeled NK cells exist in relation to the patient's tumor. The ability for repeated imaging over time with 19 F-MRI will also provide a sense of trafficking patterns as well as NK cell persistence after infusion.…”

Section: Discussionmentioning

confidence: 99%

¹⁹F-MRI for monitoring human NK cellsin vivo

et al. 2016

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The availability of clinical-grade cytokines and artificial antigen-presenting cells has accelerated interest in using natural killer (NK) cells as adoptive cellular therapy (ACT) for cancer. One of the technological shortcomings of translating therapies from animal models to clinical application is the inability to effectively and non-invasively track these cells after infusion in patients. We have optimized the nonradioactive isotope fluorine-19 ( 19

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Section: Discussionmentioning

confidence: 99%

¹⁹F-MRI for monitoring human NK cellsin vivo

et al. 2016

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“…The limitations of Fluosol PFC systems were significantly resolved by newer generation PFC compounds like perfluoroctyl bromide (also known as perflubron), perfluorodecyl bromide and perfluorodichlorooctane. 132-138 Using these compounds in specific ratios along with egg-yolk phospholipids, a PFC-based oxygen carrier named Oxygent (Alliance Pharmaceutical Corp, U.S.A.) was developed where the droplet size could be maintained at 160-180 nm in diameter, that showed reduced macrophage activation. 135,136 Intravascular circulation half-life for Oxygent droplets was found to be ~10 hr for a dose of 1.8 g/kg in humans.…”

Section: B Rbc Substitutes and Oxygen Carrier Systemsmentioning

confidence: 99%

Bio‐inspired nanomedicine strategies for artificial blood components

Gupta

2017

WIREs Nanomed Nanobiotechnol

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Blood is a fluid connective tissue where living cells are suspended in non-cellular liquid matrix. The cellular components of blood render gas exchange (RBCs), immune surveillance (WBCs) and hemostatic responses (platelets), and the non-cellular components (salts, proteins etc.) provide nutrition to various tissues in the body. Dysfunction and deficiencies in these blood components can lead to significant tissue morbidity and mortality. Consequently, transfusion of whole blood or its components is a clinical mainstay in the management of trauma, surgery, myelosuppression and congenital blood disorders. However, donor-derived blood products suffer from issues of shortage in supply, need for type matching, high risks of pathogenic contamination, limited portability and shelf-life, and a variety of side-effects. While robust research is being directed to resolve these issues, a parallel clinical interest has developed towards bioengineering of synthetic blood substitutes that can provide blood’s functions while circumventing the above problems. Nanotechnology has provided exciting approaches to achieve this, using materials engineering strategies to create synthetic and semi-synthetic RBC substitutes for enabling oxygen transport, platelet substitutes for enabling hemostasis and WBC substitutes for enabling cell-specific immune response. Some of these approaches have further extended the application of blood cell-inspired synthetic and semi-synthetic constructs for targeted drug delivery and nanomedicine. The current article will provide a comprehensive review of the various nanotechnology approaches to design synthetic blood cells, along with a critical discussion of successes and challenges of the current state-of-art in this field.

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“…The diffusion of gases into perfluorocarbon-based ultrasound contrast agents has also been discussed in other studies [18-21]. Further, the high solubility of oxygen in perfluorochemicals [22,23] and the use of perfluorochemicals as blood substitute agents have been demonstrated in previous studies [24,25]. Johnson et al [26] demonstrated that liquid PFP droplets alone can scavenge dissolved oxygen from the surrounding fluid.…”

Section: Introductionmentioning

confidence: 98%

Scavenging dissolved oxygen via acoustic droplet vaporization

Radhakrishnan

Holland

Haworth

2016

Ultrasonics Sonochemistry

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Acoustic droplet vaporization (ADV) of perfluorocarbon emulsions has been explored for diagnostic and therapeutic applications. Previous studies have demonstrated that vaporization of a liquid droplet results in a gas microbubble with a diameter 5 to 6 times larger than the initial droplet diameter. The expansion factor can increase to a factor of 10 in gassy fluids as a result of air diffusing from the surrounding fluid into the microbubble. This study investigates the potential of this process to serve as an ultrasound-mediated gas scavenging technology. Perfluoropentane droplets diluted in phosphate-buffered saline (PBS) were insonified by a 2 MHz transducer at peak rarefactional pressures lower than and greater than the ADV pressure amplitude threshold in an in vitro flow phantom. The change in dissolved oxygen (DO) of the PBS before and after ADV was measured. A numerical model of gas scavenging, based on conservation of mass and equal partial pressures of gases at equilibrium, was developed. At insonation pressures exceeding the ADV threshold, the DO of air-saturated PBS decreased with increasing insonation pressures, dropping as low as 25% of air saturation within 20 s. The decrease in DO of the PBS during ADV was dependent on the volumetric size distribution of the droplets and the fraction of droplets transitioned during ultrasound exposure. Numerically predicted changes in DO from the model agreed with the experimentally measured DO, indicating that concentration gradients can explain this phenomenon. Using computationally modified droplet size distributions that would be suitable for in vivo applications, the DO of the PBS was found to decrease with increasing concentrations. This study demonstrates that ADV can significantly decrease the DO in an aqueous fluid, which may have direct therapeutic applications and should be considered for ADV-based diagnostic or therapeutic applications.

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Untitled

Cited by 169 publications

References 24 publications

¹⁹F-MRI for monitoring human NK cellsin vivo

¹⁹F-MRI for monitoring human NK cellsin vivo

Bio‐inspired nanomedicine strategies for artificial blood components

Scavenging dissolved oxygen via acoustic droplet vaporization

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Untitled

Cited by 169 publications

References 24 publications

19F-MRI for monitoring human NK cellsin vivo

19F-MRI for monitoring human NK cellsin vivo

Bio‐inspired nanomedicine strategies for artificial blood components

Scavenging dissolved oxygen via acoustic droplet vaporization

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¹⁹F-MRI for monitoring human NK cellsin vivo

¹⁹F-MRI for monitoring human NK cellsin vivo