Abstract:Aim: The objective of this research was to obtain highly purified antirabic immunoglobulins to diagnostically significant fractions of the rabies virus antigen obtained by buoyant density separation in a step gradient of sucrose by ultracentrifugation at 30,000 g. Materials and Methods: As an immunogen, the rabies virus antigen of the "Ovechiy" GNKI strain with infectious titer Lg 10 −5.25 was used. Purification of the virus was carried out by separation in a stepwise gradient of sucrose 10-50% on Optima L-90K… Show more
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