Zarconia navalis gen. nov., sp. nov., Romeriopsis navalis gen. nov., sp. nov. and Romeriopsis marina sp. nov., isolated from inter- and subtidal environments from northern Portugal

Hentschke, Guilherme Scotta; Ramos, Vítor; Pinheiro, Ângela; Barreiro, Anabela; Costa, Mário J.; Rego, Adriana; Brûle, Sébastien; Vasconcelos, Vítor; Leão, Pedro N.

doi:10.1099/ijsem.0.005552

Cited by 7 publications

(7 citation statements)

References 41 publications

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“…LEGE 10370). These were obtained from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) from CIIMAR, Portugal [ 56 , 57 ]. Romeriopsis sp.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hydrogen Peroxide on Cyanobacterial Biofilms

Romeu,

Morais,

Vasconcelos

et al. 2023

Antibiotics

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Although a range of disinfecting formulations is commercially available, hydrogen peroxide is one of the safest chemical agents used for disinfection in aquatic environments. However, its effect on cyanobacterial biofilms is poorly investigated. In this work, biofilm formation by two filamentous cyanobacterial strains was evaluated over seven weeks on two surfaces commonly used in marine environments: glass and silicone-based paint (Sil-Ref) under controlled hydrodynamic conditions. After seven weeks, the biofilms were treated with a solution of hydrogen peroxide (H2O2) to assess if disinfection could affect long-term biofilm development. The cyanobacterial biofilms appeared to be tolerant to H2O2 treatment, and two weeks after treatment, the biofilms that developed on glass by one of the strains presented higher biomass amounts than the untreated biofilms. This result emphasizes the need to correctly evaluate the efficiency of disinfection in cyanobacterial biofilms, including assessing the possible consequences of inefficient disinfection on the regrowth of these biofilms.

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“…LEGE 10370). These were obtained from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) from CIIMAR, Portugal [ 56 , 57 ]. Romeriopsis sp.…”

Section: Methodsmentioning

confidence: 99%

Effect of Hydrogen Peroxide on Cyanobacterial Biofilms

Romeu,

Morais,

Vasconcelos

et al. 2023

Antibiotics

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“…DsyGD was identified in an Oscillatoriales isolate Zarconia navalis LEGE 11467 [41,42] (Fig. 1c, Fig.…”

Section: Gsmentioning

confidence: 99%

“…Z. navalis LEGE 11467 was obtained from the Blue Biotechnology and Ecotoxicology Culture Collection (LEGE-CC) from CIIMAR in Portugal [41] and grown with shaking at 25°C in BG-11 medium at 25 PSU (with 17.65 mM NaNO3 as a nitrogen source) as described in [66] RNA isolation from Z. navalis LEGE 11467 and cDNA synthesis Z. navalis LEGE 11467 culture pellets were resuspended in 1 mL of RNAlater RNA Stabilization Reagent (Qiagen). The suspension was incubated at room temperature for 5 min, followed by centrifugation at 2,000 g for 5 min at 4°C.…”

Section: Growth Of Zarconia Navalis Lege 11467 Under Different Condit...mentioning

confidence: 99%

“…It is unknown why this single-domain Zn DsyG had MSM activity but the truncated Gs DsyG did not. Z. navalis LEGE 11467, isolated from a subtidal epilithic marine sample [41,42] , lacked dsyD but did produce DMSP (0.385 ± 0.069 nmol DMSP mg fresh weight). These DMSP levels were much lower than for G. sunshinyii supporting our hypothesis that the double domain DsyGD enzyme was responsible for higher level production.…”

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confidence: 99%

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Novel dimethylsulfoniopropionate biosynthesis enzymes in diverse marine bacteria, cyanobacteria and abundant algae

Wang

Zhou

Curson

et al. 2023

Preprint

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Dimethylsulfoniopropionate (DMSP) is an abundant marine organosulfur compound[1] with roles in stress protection[2, 3], chemotaxis[4], nutrient and sulfur cycling[5] and, potentially, climate regulation[6, 7]. Marine algae and bacteria are considered significant DMSP producers, but many diverse representatives lack known DMSP synthesis genes/enzymes[8, 9]. Here, new DMSP biosynthesis enzymes were identified that considerably increase the number and diversity of potential DMSP-producing organisms, inferring new and significant global DMSP producers. A novel bifunctional DMSP biosynthesis enzyme, DsyGD, identified in the rhizobacterium Gynuella sunshinyii, produces DMSP at levels higher than any other bacterium from methylthiohydroxybutyrate (MTHB) via an N-terminal MTHB S-methyltransferase domain (termed DsyG) and a C-terminal dimethylsulfoniohydroxybutyrate (DMSHB) decarboxylase domain (termed DsyD, which is the first reported enzyme with this activity). DsyGD is also found in some filamentous cyanobacteria, not previously known to produce DMSP. Regulation of DMSP production and dsyGD transcription was consistent with their role in osmoprotection. Indeed, cloned dsyGD conferred osmotolerance to bacteria deficient in osmolyte production, something not previously demonstrated for any known DMSP synthesis gene, and which could be exploited for biotechnology e.g., engineering salt tolerance. DsyGD characterisation led to identification of phylogenetically distinct DsyG-like proteins, termed DSYE, with MTHB S-methyltransferase activity, in diverse and environmentally abundant Chlorophyta, Chlorachniophyta, Ochraphyta, Haptophyta and Bacillariophyta algae. These algae comprise a mix of low, high and previously unknown DMSP producers[10]. Algae containing DSYE, particularly bloom-forming Pelagophyceae species, which we showed to accumulate medium-high intracellular DMSP levels, were globally more abundant DMSP producers than Haptophyta, Dinophyta and Bacillariophyta with DSYB and/or TpMMT. This highlights the potential importance of Pelagophyceae and other DSYE containing algae in global DMSP production and sulfur cycling.

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“…This goes beyond modeling studies, from the collection of data under an integrated surveillance system framework, to its analysis, to informing the implementation of policies and interventions, also beyond the health sector. The One Health concept stands out for fostering this kind of joint work highlighting collaborative efforts across multiple disciplines and sectors at local, national, regional, and global levels with the goal of achieving optimal health outcomes for people, animals, and our shared environment [178]. Clearly, addressing the research needs discussed in the previous section will require adopting this transdisciplinary thinking to prevent siloed approaches and increase communication and information sharing between experts from different fields.…”

Section: Fostering Transdisciplinary Thinkingmentioning

confidence: 99%

West Nile virus eco-epidemiology and climate change

2023

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West Nile virus (WNV) is an arbovirus with a wide geographical distribution. It is maintained in an enzootic bird-mosquito cycle and exhibits regular zoonotic transmission to humans, to whom it can cause West Nile fever and neuroinvasive disease. Over the last decades, WNV has emerged as a serious health threat with profound impacts on animal and human health. It is broadly accepted that climate change impacts the transmission of WNV through multiple pathways via its impacts on vectors, the pathogen, hosts, and the environment. Although some clarity has been established, the outcomes of these climate-driven processes are yet to be better understood before they can fully benefit tailored prevention and control measures. This review focuses on the eco-epidemiology of WNV and how it is impacted by climate change through an integrative lens. We discuss the role of eco-epidemiological modeling in knowledge generation and highlight the importance of adopting an interdisciplinary and transdisciplinary One Health approach for improved surveillance, data collection, modeling, and translation of outcomes into actionable policy and control.

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Zarconia navalis gen. nov., sp. nov., Romeriopsis navalis gen. nov., sp. nov. and Romeriopsis marina sp. nov., isolated from inter- and subtidal environments from northern Portugal

Cited by 7 publications

References 41 publications

Effect of Hydrogen Peroxide on Cyanobacterial Biofilms

Effect of Hydrogen Peroxide on Cyanobacterial Biofilms

Novel dimethylsulfoniopropionate biosynthesis enzymes in diverse marine bacteria, cyanobacteria and abundant algae

West Nile virus eco-epidemiology and climate change

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