Zastosowanie metod biologii molekularnej do identyfikacji grzybów zasiedlających martwe drewno sosny zwyczajnej (Pinus Sylvestris L.)

Abstract

The aim of this study was to evaluate the biodiversity of fungi, found in the dead wood of pine. The material for the study was divided into three individual stages of wood decomposition. The analysed wood samples were obtained from two areas in managed forest and protected area. Fungal communities were selected using a molecular method, which based on polymerase chain reaction (PCR), cloned and sequenced by Sangar. The results have shown that the species composition of fungi communities was varied, depending on the stage of wood decomposition. The most numerous was phylum Ascomycota.