Zebrafish: A novel model organism to assess probiotics influence on growth and development

Rajakumari, Devarapogu; Viswanath, Buddolla; Rani, A. Usha

doi:10.1016/b978-0-12-821406-0.00029-1

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…Indeed, germ-free larvae of zebrafish immersed with Lactobacillus fermentum NA4 displayed an increased il10 gene expression and a decreased il1β and tnfα gene expression after chemically induced inflammation compared to controls. However, in the same study, larvae immersed with several strains of Lactobacillus plantarum (WCFS1 and NA7) or other Lactobacillus fermentum strains (ATCC9338 and NA6) did not show differences in the expression of the same genes [53]. However, our study obtained a different pattern of gene expression for LHPro, LHPost and LPPost, underlining that not only the strain but the culturing process of production for both probiotic and postbiotics may affect the impact of the microbial product on innate effector cytokine expression profiles.…”

Section: Discussionmentioning

confidence: 77%

“…Moreover, lysozyme is highly conserved in the animal kingdom and plays an important role in the protection of the host from potential bacterial and viral infection. Today, there are only a limited number of studies that compare the capacity of candidate probiotic/postbiotic strains to induce such peptides' expression, and much of the work has been conducted in vitro, notably using the Caco-2 epithelial cell line [50][51][52][53]. Despite this, in healthy adult male zebrafish, lyz together with tnfα gene expression increased in groups fed with live Lactobacillus casei probiotic compared to the control, interestingly in a dose-response manner from 10 5 to 10 7 CFU/g feed.…”

Section: Discussionmentioning

confidence: 99%

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Modulation of Zebrafish (Danio rerio) Intestinal Mucosal Barrier Function Fed Different Postbiotics and a Probiotic from Lactobacilli

Rawling,

Schiavone,

Mugnier

et al. 2023

Microorganisms

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It is generally accepted that microbes play a critical role in maintaining gut barrier function, making them ideal to target in order to mitigate the effects of intestinal diseases such as inflammatory bowel disease with specialist supplementations such as probiotic or postbiotic preparations. In this study, specific strains of Lactobacillus helvictus both live and inactivated and Lactobacillus plantarum inactivated were fed to zebrafish at an inclusion level of 6 × 106 cells/g in order to assess the effects on gut barrier function and protection. Taken together, our results indicate that dietary administration of pro- or postbiotics strengthens the gut barrier function and innate immunity of healthy zebrafish in a strain-specific and process-dependent way. With some differences in the response intensity, the three treatments led to increased intestinal villi length and proportion of IELs, reinforcement of the GC population and up-regulated expression of biomarkers of AMP production and tight junction zona-occludin 2a (zo-2a). In addition, LPPost had an impact on the adaptive immune response, and we hypothesized that it conferred the potential to drive Th17/ILC3 immunity, as suggested by its effect on the gene expression of il22, of different AMPs, and the expression of zo2a. Moreover, LPPost showed the potential to drive Th1/ILC1-like immunity, with a higher percentage of CD8+ cells and higher ifnγ gene expression. In summary, the use of inactivated Lactobacilli species in this study represented a promising strategy for improving barrier function and regulating the immune fate of the intestinal mucosa in a strain-specific way.

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Section: Discussionmentioning

confidence: 77%