Zebrafish anterior segment mesenchyme progenitors are defined by function of tfap2a but not sox10

Vöcking, Oliver; Meulen, K Van Der; Patel, M.K; Famulski, Jakub K.

doi:10.1016/j.diff.2022.11.002

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…The expression of FOXD3 and SOX10 mostly overlapped at every stage tested, although the expression of FOXD3 seemed to be weaker on day 5 than on day 4. In model animals, such as chicks and zebrafish, FOXD3 and SOX10 are expressed in distinct but partially overlapping patterns, and FOXD3 seems to be downregulated as development proceeds 15 , 16 . Weaker expression of FOXD3 at day 5 may reflect the downregulation observed in vivo.…”

Section: Resultsmentioning

confidence: 99%

In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells

Seto,

Ogihara,

Takizawa

et al. 2024

Nat Commun

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Early patterning of neural crest cells (NCCs) in the craniofacial primordium is important for subsequent development of proper craniofacial structures. However, because of the complexity of the environment of developing tissues, surveying the early specification and patterning of NCCs is difficult. In this study, we develop a simplified in vitro 3D model using human pluripotent stem cells to analyze the early stages of facial development. In this model, cranial NCC-like cells spontaneously differentiate from neural plate border-like cells into maxillary arch-like mesenchyme after a long-term culture. Upon the addition of EDN1 and BMP4, these aggregates are converted into a mandibular arch-like state. Furthermore, temporary treatment with EDN1 and BMP4 induces the formation of spatially separated domains expressing mandibular and maxillary arch markers within a single aggregate. These results suggest that this in vitro model is useful for determining the mechanisms underlying cell fate specification and patterning during early facial development.

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Section: Resultsmentioning

confidence: 99%

In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells

Seto,

Ogihara,

Takizawa

et al. 2024

Nat Commun

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“…We have recently shown that ASM cells labelled in Tg[foxc1b:GFP] migrate into the AS space and contain transcriptomic profiles consistent with being AS progenitors 39 . The foxc1 transcription factor is also a well-known regulator and marker of the AS 39,43 . To confirm that Tg[foxc1b:GFP] GFP+ cells can serve as reliable sources of ASM cell thorough AS development we assayed their expression patterns over AS development from 24-144 hpf (Fig.…”

Section: Resultsmentioning

confidence: 99%

A temporal single cell transcriptome atlas of zebrafish anterior segment development

Vöcking

Famulski

2023

Sci Rep

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Anterior segment dysgenesis (ASD), resulting in vision impairment, stems from maldevelopment of anterior segment (AS) tissues. Incidence of ASD has been linked to malfunction of periocular mesenchyme cells (POM). POM cells specify into anterior segment mesenchyme (ASM) cells which colonize and produce AS tissues. In this study we uncover ASM developmental trajectories associated with formation of the AS. Using a transgenic line of zebrafish that fluorescently labels the ASM throughout development, Tg[foxc1b:GFP], we isolated GFP+ ASM cells at several developmental timepoints (48–144 hpf) and performed single cell RNA sequencing. Clustering analysis indicates subdifferentiation of ASM as early as 48 hpf and subsequent diversification into corneal epithelium/endothelium/stroma, or annular ligament (AL) lineages. Tracking individual clusters reveals common developmental pathways, up to 72 hpf, for the AL and corneal endothelium/stroma and distinct pathways for corneal epithelium starting at 48 hpf. Spatiotemporal validation of over 80 genes found associated with AS development demonstrates a high degree of conservation with mammalian trabecular meshwork and corneal tissues. In addition, we characterize thirteen novel genes associated with annular ligament and seven with corneal development. Overall, the data provide a molecular verification of the long-standing hypothesis that POM derived ASM give rise to AS tissues and highlight the high degree of conservation between zebrafish and mammals.

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“…From 14 hpf, mesenchymal cells exhibiting cytoplasmic Laminin-sfGFP were seen to migrate anteriorly around the OP (Movie 1). According to the literature, these cells could represent neural crest cells (NCC) (Bryan et al, 2020 ;Harden et al, 2012 ;Torres-Paz and Whitlock, 2014 ) and/or mesodermally-derived cells of the periocular mesenchyme (Vöcking et al, 2023 ). A subset of RFP+ OP cells also displayed cytoplasmic Laminin-sfGFP (Fig.…”

Section: The Developing Op and Brain Tissues Are Surrounded By Lamini...mentioning

confidence: 99%

Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development

Tignard,

Pottin,

Geeverding

et al. 2024

eLife

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Despite recent progress, the complex roles played by the extracellular matrix in development and disease are still far from being fully understood. Here, we took advantage of the zebrafish sly mutation which affects Laminin γ1, a major component of basement membranes, to explore its role in the development of the olfactory system. Following a detailed characterisation of Laminin distribution in the developing olfactory circuit, we analysed basement membrane integrity, olfactory placode and brain morphogenesis, and olfactory axon development in sly mutants, using a combination of immunochemistry, electron microscopy and quantitative live imaging of cell movements and axon behaviours. Our results point to an original and dual contribution of Laminin γ1-dependent basement membranes in organising the border between the olfactory placode and the adjacent brain: they maintain placode shape and position in the face of major brain morphogenetic movements, they establish a robust physical barrier between the two tissues while at the same time allowing the local entry of the sensory axons into the brain and their navigation towards the olfactory bulb. This work thus identifies key roles of Laminin γ1-dependent basement membranes in neuronal tissue morphogenesis and axon development in vivo.

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Zebrafish anterior segment mesenchyme progenitors are defined by function of tfap2a but not sox10

Cited by 8 publications

References 34 publications

In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells

In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells

A temporal single cell transcriptome atlas of zebrafish anterior segment development

Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development

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