Zebrafish as a model system to study toxicology

Dai, Yujie; Jia, Yongfang; Chen, Na; Bian, Wan-Ping; Li, Qinkai; Ma, Yanbo; Chen, Yanling; Pei, De‐Sheng

doi:10.1002/etc.2406

Cited by 460 publications

(224 citation statements)

References 89 publications

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“…Most fish of this species available in the market are imported from South-East Asia, where they are raised in the aquarium fish farms and in special laboratories or collected from the wild. The zebrafish has many advantages as a model organism, such as small size, reaching maturity in about three months, short reproductive cycle, easy breeding, transparent embryos and many others as possibility to spawn through the year under controlled conditions (Gioacchini et al, 2010;Dai et al, 2014). The zebrafish genome shows a high level of homology with human genome, so the importance of this species as a model species is highly increasing (Dai et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Kucharczyk¹,

Stepien²,

Nowosad³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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Zebrafish is popular aquarium fish and laboratory model species but some aspects of advanced spawning technologies, especially of reproduction and rearing in low temperatures (>25°C), which involve the production of high quality and viable gametes, should be studied. In the present study fish before spawning were reared at cold thermal regime: 19-20°C. From three tested temperatures for fish spawning, the highest spawning effectiveness was noted at 23°C. The highest rate of embryo survival was noted when the temperature in which the spawners were kept before spawning was the lowest (19°C). In the spawning of small breeders groups, the number of males should be higher than the number of females. If the sex rate was 1:1, the lowest embryo survival was noted. From the breeding perspective fish of this species should be reproduced again shortly after the completed spawning a time between spawns should be 20 to 60 days if the spawners were kept at 19°C. Keeping the fish between spawning periods more than 40 days results in a significant deterioration of quality of gametes, expressed as embryo survival. It was observed that spawners of this species produce viable gametes and spawn successfully every 20 days, during a few following spawning periods without changes of embryo survival.

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Section: Introductionmentioning

confidence: 99%

Untitled

Kucharczyk¹,

Stepien²,

Nowosad³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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“…The generation of mutant and transgenic fish lines opened up new possibilities for zebrafish research, and novel genome-editing techniques like CRISPR/Cas9 will further advance the field. As a result, over the last decade, the zebrafish has emerged as an important model system not only for research on vertebrate development (Beis & Stainier 2006, Collins & Stainier 2016 but also for ecotoxicological (Dai et al 2014) and biomedical research (Lieschke & Currie 2007, MacRae & Peterson 2015. In this review, an overview is presented of the research on NRs using the zebrafish as a model system.…”

Section: Introductionmentioning

confidence: 99%

Nuclear receptor research in zebrafish

Schaaf

2017

Journal of Molecular Endocrinology

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Nuclear receptors (NRs) form a superfamily of transcription factors that can be activated by ligands and are involved in a wide range of physiological processes. NRs are well conserved between vertebrate species. The zebrafish, an increasingly popular animal model system, contains a total of 73 NR genes, and orthologues of almost all human NRs are present. In this review article, an overview is presented of NR research in which the zebrafish has been used as a model. Research is described on the three most studied zebrafish NRs: the estrogen receptors (ERs), retinoic acid receptors (RARs) and peroxisome proliferator-activated receptors (PPARs). The studies on these receptors illustrate the versatility of the zebrafish as a model for ecotoxicological, developmental and biomedical research. Although the use of the zebrafish in NR research is still relatively limited, it is expected that in the next decade the full potential of this animal model will be exploited.

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“…Acid guanidinium thiocyanate phenol chloroform extraction is widely used for extraction and isolation of pure RNA from different tissue samples of rodents and humans (Ma et al, 2008). Zebrafish is a powerful model organism currently used in molecular biology, toxicology, environmental monitoring and cancer research (Dai et al, 2014). Methods are available for the extraction of RNA from zebrafish embryos but no specific method for obtaining RNA from tissue.…”

Section: Introductionmentioning

confidence: 99%

A novel method to achieve high yield of total RNA from zebrafish for expression studies

Shukla

Jhamtani

Dahiya

et al. 2017

IJB

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Gene expression studies require high-quality intact total RNA for real-time PCR analysis. Degraded or impure sample can provide disastrous results in the subsequence process. RNA is very unstable and can be contaminated easily by ribonuclease (RNase), therefore utmost quality control checks are required to ensure best quality RNA with high yield. Herein, we have come out with a protocol to isolate total RNA from liver, kidney and brain of adult zebrafish using a commercially available chemical denaturant and subsequent clean-up to remove traces of DNA and impurities. Kidney tissue gives maximum yield of total RNA concentration i.e. 135.8786 ng/µL. Purity of isolated RNA was observed with an absorbance ranging from 1.9 to 2.0 observed in all samples. The whole procedure was repeatedly performed to get satisfactory results. Throughout these procedures there are numerous quality control checks to ensure that the sample is neither degraded nor contaminated.

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Zebrafish as a model system to study toxicology

Cited by 460 publications

References 89 publications

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Nuclear receptor research in zebrafish

A novel method to achieve high yield of total RNA from zebrafish for expression studies

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