Zebrafish as an emerging model to study gonad development

Ye, Mengling; Chen, Ye

doi:10.1016/j.csbj.2020.08.025

Cited by 29 publications

(19 citation statements)

References 105 publications

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“…Future experiments to elucidate possible roles for Cd9b in these processes could include histological analysis of the gonads at stages throughout development, analysis of gametogenesis and reproductive hormones such as follicular stimulating hormone and luteinizing hormone in sexually mature zebrafish, as well as investigating the expression of genes known to play a role in gonad development (e.g. ar , cyp11c1 , cyp17a1 , fancl , foxl2 , hsf5 , piwil1 , piwil2) [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

Tetraspanin Cd9b plays a role in fertility in zebrafish

et al. 2022

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In mice, CD9 expression on the egg is required for efficient sperm-egg fusion and no effects on ovulation or male fertility are observed in CD9 null animals. Here we show that cd9b knockout zebrafish also appear to have fertility defects. In contrast to mice, fewer eggs were laid by cd9b knockout zebrafish pairs and, of the eggs laid, a lower percentage were fertilised. These effects could not be linked to primordial germ cell numbers or migration as these were not altered in the cd9b mutants. The decrease in egg numbers could be rescued by exchanging either cd9b knockout partner, male or female, for a wildtype partner. However, the fertilisation defect was only rescued by crossing a cd9b knockout female with a wildtype male. To exclude effects of mating behaviour we analysed clutch size and fertilisation using in vitro fertilisation techniques. Number of eggs and fertilisation rates were significantly reduced in the cd9b mutants suggesting the fertility defects are not solely due to courtship behaviours. Our results indicate that CD9 plays a more complex role in fish fertility than in mammals, with effects in both males and females.

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Section: Resultsmentioning

confidence: 99%

Tetraspanin Cd9b plays a role in fertility in zebrafish

et al. 2022

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“…Instead, the F1 effects could be attributed to direct exposure to the germ line. At 17 dpf in zebrafish, perinuclear oocytes appear with gonad differentiation occurring between 21 and 40 dpf, at which point germ cells are present at multiple stages of oogenesis in female gonads, and maturation continues until 90 dpf [ 37 ]. Therefore, germ cells destined to become the F1 generation were present and exposed during the period that the F0 juveniles were fed a contaminated diet.…”

Section: Discussionmentioning

confidence: 99%

“…At 17 dpf, the juvenile zebrafish were transferred to 8 tanks of 9 L capacity at densities of 120 fish per tank and put onto an isolated intermittent flow system to facilitate the dietary exposure. Fish were fed the control or PFHxA-contaminated diets from 17 to 59 dpf, a total of 42 days encompassing critical windows of gonad development ( Figure 1 ) [ 37 ]. From 17 to 30 dpf, exposure groups in two tanks each ( n = 240 fish total) were fed the control or contaminated GEMMA Micro 75 diets.…”

Section: Methodsmentioning

confidence: 99%

Dietary Perfluorohexanoic Acid (PFHxA) Exposures in Juvenile Zebrafish Produce Subtle Behavioral Effects across Generations

Rericha

Truong

Leong

et al. 2022

Toxics

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Ubiquitous anthropogenic contaminants of concern, per- and polyfluoroalkyl substances (PFAS) are frequently detected in the environment and human populations around the world. Diet is a predominate route of human exposure, and PFAS are frequently measured in food. Manufacturing trends have shifted from legacy PFAS to shorter-chain alternatives that are suggested to be safer, such as perfluorohexanoic acid (PFHxA). However, the current amount of data to support safety assessments of these alternatives is not yet sufficient. The present study investigated the effects of a 42-day dietary exposure to 1, 10, or 100 ng/g PFHxA in juvenile zebrafish. The zebrafish model was leveraged to interrogate morphometrics, fecundity, and numerous behavior endpoints across multiple generations. Dietary PFHxA exposure did not result in measurable body burden and did not affect growth, fecundity, adult social perception behavior, or associative learning. PFHxA exposure did induce abnormal adult anxiety behaviors in the F0 generation that persisted transgenerationally in the F1 and F2. Abnormal larval and juvenile behavior was observed in the F1 generation, but not in the F2. PFHxA juvenile dietary exposure induced subtle and multigenerational behavior effects that warrant further investigation of this and other alternative short-chain PFAS.

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“…Additionally, the exposures performed in this study ended at 120 hpf, prior to key reproductive events such as sex differentiation and production of germ cells. The lack of bioactivity we observed with exposure to DEHP and DEP may be due to the target tissues not yet being present at 5 dpf, or the effects may simply be unobservable until later in life [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals

Rivera

Wilson

Kim

et al. 2022

IJERPH

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A 2019 retrospective study analyzed wristband personal samplers from fourteen different communities across three different continents for over 1530 organic chemicals. Investigators identified fourteen chemicals (G14) detected in over 50% of personal samplers. The G14 represent a group of chemicals that individuals are commonly exposed to, and are mainly associated with consumer products including plasticizers, fragrances, flame retardants, and pesticides. The high frequency of exposure to these chemicals raises questions of their potential adverse human health effects. Additionally, the possibility of exposure to mixtures of these chemicals is likely due to their co-occurrence; thus, the potential for mixtures to induce differential bioactivity warrants further investigation. This study describes a novel approach to broadly evaluate the hazards of personal chemical exposures by coupling data from personal sampling devices with high-throughput bioactivity screenings using in vitro and non-mammalian in vivo models. To account for species and sensitivity differences, screening was conducted using primary normal human bronchial epithelial (NHBE) cells and early life-stage zebrafish. Mixtures of the G14 and most potent G14 chemicals were created to assess potential mixture effects. Chemical bioactivity was dependent on the model system, with five and eleven chemicals deemed bioactive in NHBE and zebrafish, respectively, supporting the use of a multi-system approach for bioactivity testing and highlighting sensitivity differences between the models. In both NHBE and zebrafish, mixture effects were observed when screening mixtures of the most potent chemicals. Observations of BMC-based mixtures in NHBE (NHBE BMC Mix) and zebrafish (ZF BMC Mix) suggested antagonistic effects. In this study, consumer product-related chemicals were prioritized for bioactivity screening using personal exposure data. High-throughput high-content screening was utilized to assess the chemical bioactivity and mixture effects of the most potent chemicals.

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Zebrafish as an emerging model to study gonad development

Cited by 29 publications

References 105 publications

Tetraspanin Cd9b plays a role in fertility in zebrafish

Tetraspanin Cd9b plays a role in fertility in zebrafish

Dietary Perfluorohexanoic Acid (PFHxA) Exposures in Juvenile Zebrafish Produce Subtle Behavioral Effects across Generations

A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals

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