Zebrafish (Danio rerio) TRβ- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols

Antagonism of Metal Pyridinethiones and Their Degradation Products toward Estrogen Receptor α: A Combination of In Vitro, In Vivo, and In Silico Approaches

“…The E2 concentrations were plotted against the corresponding differential pulse voltammetry (DPV) peak currents, resulting in a standard curve. E2 equivalent concentrations of MePT and their degradation products were calculated by applying this linear regression equation, and the relative binding potencies of MePT and their degradation products with ERα were expressed as E2 equivalents, i.e., at the same level (taken as 10 –7 mol/L), E2 equivalent concentrations divided by E2 concentration …”

“…The binding potencies of MePTs and their degradation products with ERα were analyzed using an ERα-based electrochemical biosensor, which was developed using a CHI660D electrochemical workstation with a three-electrode system (platinum counter electrode and Ag/AgCl reference electrode) (CH Instruments, Shanghai, China), according to our published study (Supporting Information). 26 Serial dilutions of MePTs and their degradation products from 10 −11 to 10 −4 mol/L were added to the fabricated electrode. A blank control, a DMSO solvent control, and E2 positive control groups (10 −15 to 10 −4 mol/L) completed the exposure design.…”

“…E2 equivalent concentrations of MePT and their degradation products were calculated by applying this linear regression equation, and the relative binding potencies of MePT and their degradation products with ERα were expressed as E2 equivalents, i.e., at the same level (taken as 10 −7 mol/L), E2 equivalent concentrations divided by E2 concentration. 26 2.3. Two-Hybrid Yeast Assay and MCF-7 Cell Proliferation Assay.…”