Zebrafish <i>znfl1s</i> regulate left‐right asymmetry patterning through controlling the expression of <i>fgfr1a</i>

Li, Jingyun; Gao, Feng; Zhao, Yanling; He, Luqingqing; Huang, Yun; Yang, Xiaojing; Zhou, Yahui; Yu, Lingling; Zhao, Qingshun; Dong, Xiaohua

doi:10.1002/jcp.27564

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…While other transcription factors such as gata4 ( 29 ), mef2ca ( 30 ), and tbx20 ( 31 ) form a key regulatory network indispensable to maintain the normal cardiac development. In addition, the heart is one of the earliest internal LR asymmetric organs with conserved regulatory mechanism across different species ( 32 , 33 ). For example, the nodal pathway plays a conserved role in LR asymmetric morphogenesis through establishing initial molecular differences.…”

Section: Discussionmentioning

confidence: 99%

An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis

Ouyang

Qin

Niu

et al. 2022

Front. Cardiovasc. Med.

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BackgroundDrug exposure during gestation or in prematurely born children represents a significant risk to congenital heart disease (CHD). Amantadine is an antiviral agent also effective in the treatment of Parkinson’s disease. However, while its potential side effects associated with tetralogy of fallot (ToF) and birth defects were implicated, its underlying etiologic mechanisms of action remain unknown. Here, we report teratogenic effects of amantadine drug during early cardiogenesis through developing a novel zebrafish (Danio rerio) knock-in (KI) animal model and explore the underlying mechanisms.MethodsHomologous recombination (HR) pathway triggered by CRISPR/Cas9 system was utilized to generate an enhanced green fluorescent protein (EGFP) KI zebrafish animal model. Dynamic fluorescence imaging coupled with a whole-mount in-situ hybridization (WISH) assay was employed to compare the spatial and temporal expression patterns of the EGFP reporter in the KI animal model with the KI-targeted endogenous gene. Heart morphology and EGFP expression dynamics in the KI animal models were monitored to assess cardiac side effects of different doses of amantadine hydrochloride. Expression of key genes required for myocardium differentiation and left–right (LR) asymmetry was analyzed using WISH and quantitative reverse transcription-PCR (RT-PCR).ResultsA novel EGFP KI line targeted at the ventricular myosin heavy chain (vmhc) gene locus was successfully generated, in which EGFP reporter could faithfully recapitulate the endogenous expression dynamics of the ventricle chamber-specific expression of the vmhc gene. Amantadine drug treatment-induced ectopic expression of vmhc gene in the atrium and caused cardiac-looping or LR asymmetry defects to dose-dependently during early cardiogenesis, concomitant with dramatically reduced expression levels of key genes required for myocardium differentiation and LR asymmetry.ConclusionWe generated a novel zebrafish KI animal model in which EGFP reports the ventricle chamber-specific expression of vmhc gene dynamics that is useful to effectively assess drug safety on the cardiac morphology in vivo. Specifically, this study identified teratogenic effects of amantadine drug during early cardiogenesis dose dependent, which could be likely conveyed by inhibiting expression of key genes required for cardiac myocardium differentiation and LR asymmetry.

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Section: Discussionmentioning

confidence: 99%

An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis

Ouyang

Qin

Niu

et al. 2022

Front. Cardiovasc. Med.

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“…lncrps25 MO1 sequences is GAAACCAATGCGCTACT TACTTTGC, and lncrps25 MO2 sequences is GGCCTTCTGTAATG TACAGTTATTT. The sequences of control MO is CCTCTTACCTC AGTTACAATTTATA (Dong et al, 2017;Li et al, 2019).…”

Section: Microinjection Of Morpholino Oligonucleotides (Mos) Into Zebrafish Embryosmentioning

confidence: 99%

lncrps25 play an essential role in motor neuron development through controlling the expression of olig2 in zebrafish

Gao

et al. 2019

Journal Cellular Physiology

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lncrps25 is an intergenic long noncoding RNA (lncRNA), which is location close to rps25 (ribosomal protein S25) gene, is reported share high conserved sequence with NREP (neuronal regeneration-related protein) 3′-untranslated region. The function and mechanism of most of the lncRNA in embryo development remain largely unknown. In zebrafish, lncrps25 is widely expressed in the early embryonic stage and spinal cord during development. Morpholino (MO) knockdown of zebrafish lncrps25

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Research Progress and Direction of Novel Organelle—Migrasomes

Zhang

Xie

Ding

et al. 2022

Cancers

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Migrasomes are organelles that are similar in structure to pomegranates, up to 3 μm in diameter, and contain small vesicles with a diameter of 50–100 nm. These membranous organelles grow at the intersections or tips of retracting fibers at the back of migrating cells. The process by which cells release migrasomes and their contents outside the cell is called migracytosis. The signal molecules are packaged in the migrasomes and released to the designated location by migrasomes to activate the surrounding cells. Finally, the migrasomes complete the entire process of information transmission. In this sense, migrasomes integrate time, space, and specific chemical information, which are essential for regulating physiological processes such as embryonic development and tumor invasion and migration. In this review, the current research progress of migrasomes, including the discovery of migrasomes and migracytosis, the structure of migrasomes, and the distribution and functions of migrasomes is discussed. The migratory marker protein TSPAN4 is highly expressed in various cancers and is associated with cancer invasion and migration. Therefore, there is still much research space for the pathogenesis of migratory bodies and cancer. This review also makes bold predictions and prospects for the research directions of the combination of migrasomes and clinical applications.

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Zebrafish znfl1s regulate left‐right asymmetry patterning through controlling the expression of fgfr1a

Cited by 3 publications

References 38 publications

An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis

An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis

lncrps25 play an essential role in motor neuron development through controlling the expression of olig2 in zebrafish

Research Progress and Direction of Novel Organelle—Migrasomes

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