Zebrafish models: do we have valid paradigms for depression?

Abreu, Murilo S. de; Friend, Ashton J.; Demin, Konstantin A.; Amstislavskaya, Tamara G.; Bao, Wandong; Kalueff, Allan V.

doi:10.1016/j.vascn.2018.07.002

Cited by 36 publications

(24 citation statements)

References 107 publications

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“…Given problems with data reliability and replicability in the field 74 , the choice of prolonged stress protocol, such as PUCS developed here, seems to be justified. Finally, while chronic stress models are widely interpreted as models of affective disorders, including depression, it is still unclear whether there is a clear-cut depression-like phenotype in the zebrafish 75 . Given the constantly evolving, and sometimes opposite, behavioral patterns evoked by chronic stress in many neurobehavioral domains (except anxiety) 26 , 27 , further studies are needed to better understand the exact interplay between these factors in CUS, necessitating further protocols with differing numbers of stressors, their severity, and modeling duration.…”

Section: Discussionmentioning

confidence: 99%

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Demin

Колесникова

Galstyan

et al. 2021

Sci Rep

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Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS + EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism.

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Section: Discussionmentioning

confidence: 99%

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Demin

Колесникова

Galstyan

et al. 2021

Sci Rep

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“…It seems that the most important problem that emerges when studying depression in Danio rerio is the inability to appropriately distinguish differences between the locomotor inactivity and the anxiety-like from the depressive-like state. As indicated in the review by de Abreu et al [ 133 ], a more detailed analysis of fish behavior during a given experiment is necessary. As the authors highlighted, in order to correctly classify the depressive- and anxiety-like behavior of zebrafish in the behavioral tests described above, many parameters must be taken into account at the same time.…”

Section: Zebrafish In Neurological Studiesmentioning

confidence: 99%

“…In order to increase the certainty of whether the depressive-, anxiety-, or depressive-anxiety-like behavior have occurred in Danio rerio , it is important to check whether reactions of the tested subjects can be reversed by administration of antidepressant and/or anxiolytic treatment. For example, in the NTT, zebrafish displaying hypolocomotion and maintaining an up/down preference may be treated as subjects with the typical depressive-like behavior, whereas a reduced motor activity accompanied by freezing episodes, irregular movements, and/or a preference for swimming in a lower part of the tank may be specific to the anxiety-like behavior [ 133 ]. However, as indicated by de Abreu et al [ 133 ], this cannot be taken for granted.…”

Section: Zebrafish In Neurological Studiesmentioning

confidence: 99%

“…For example, in the NTT, zebrafish displaying hypolocomotion and maintaining an up/down preference may be treated as subjects with the typical depressive-like behavior, whereas a reduced motor activity accompanied by freezing episodes, irregular movements, and/or a preference for swimming in a lower part of the tank may be specific to the anxiety-like behavior [ 133 ]. However, as indicated by de Abreu et al [ 133 ], this cannot be taken for granted. Therefore, it seems a good solution to check whether the observed behavioral changes in fish improve after introduction of conventional antidepressants and/or anxiolytics.…”

Section: Zebrafish In Neurological Studiesmentioning

confidence: 99%

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Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential

Lachowicz

Niedziałek

Rostkowska³

et al. 2021

Life

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Depression is a serious mental disease that, according to statistics, affects 320 million people worldwide. Additionally, a current situation related to the COVID-19 pandemic has led to a significant deterioration of mental health in people around the world. So far, rodents have been treated as basic animal models used in studies on this disease, but in recent years, Danio rerio has emerged as a new organism that might serve well in preclinical experiments. Zebrafish have a lot of advantages, such as a quick reproductive cycle, transparent body during the early developmental stages, high genetic and physiological homology to humans, and low costs of maintenance. Here, we discuss the potential of the zebrafish model to be used in behavioral studies focused on testing agents with antidepressant potential.

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“…Therefore, when choosing a stress model, one is guided by such biological objects in which cortisol is an indicator of stress. Among laboratory animals, these include fish, in particular Zebrafish, or Danio rerio, a wild type found in shallow waters of Southeast Asia and actively reproduced as an aquarium animal (Menke et al 2001, Griffiths et al 2012, De Abreu et al 2018. A feature of Danio rerio is also the non-isolation of individual genetic lines of the species, but the use of exclusively wild-type animals as a biological object for laboratory research.…”

Section: Introductionmentioning

confidence: 99%

In search of new brain biomarkers of stress

Shabanov¹,

Blazhenko²,

Devyashin³

et al. 2021

RRP

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The aim: of the study was to investigate the level of ghrelin in various brain structures during a stress response in Zebrafish to a predator, to evaluate this indicator as a potential biomarker of stress, and the effect of a benzodiazepine tranquilizer (phenazepam) on stress-induced changes Materials and methods: The object of the study was Zebrafish, or Danio rerio wild type, which was subjected to stress by exposure to a predator Hypsophrys nicaraguensis from the cichlid family. In the tail tissue, the level of cortisol was determined, in the brain – the level of total (acylated and non-acylated) ghrelin by the method of enzyme-linked immunosorbent assay. The benzodiazepine anxiolytic phenazepam (1 mg/L), a ghrelin antagonist [D-Lys3]-GHRP-6 (0.333 mg/l) and corticotropin-releasing hormone (CRF; 0.4 mg/L) were used as the pharmacological agents. Results and discussion: Exposure to a predator, just as administering CRF, more than doubled the level of cortisol in the tail tissue. [D-Lys3]-GHRP-6 and phenazepam prevented an increase in a tissue cortisol level. Simultaneously, in the medulla oblongata and cerebellum, the phylogenetically most ancient structures, rather than in the forebrain (telencephalon) or in the midbrain (corpora bigemia), the level of ghrelin was recorded about 500 pg/g of total protein. In response to exposure to a predator, the level of ghrelin increased in the forebrain and midbrain to nanogram concentrations and moderately decreased in the cerebellum. The effect was prevented by phenazepam and [D-Lys3]-GHRP-6. Conclusion: Increases in ghrelin in the brain in response to stressful situations can be seen as a functional brain biomarker of stress, along with increased levels of tissue cortisol levels. Both of these effects are prevented by both the ghrelin antagonist and the benzodiazepine tranquilizer. The mechanism of action of the tranquilizer is a functional antagonism between the GABAergic system of the brain and the ghrelin system.

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Zebrafish models: do we have valid paradigms for depression?

Cited by 36 publications

References 107 publications

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential

In search of new brain biomarkers of stress

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