Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development

Liu, Yu-Hsiu; Lin, Tz-Chi; Hwang, Sheng-Ping L.

doi:10.1016/j.mod.2020.103598

Cited by 15 publications

(10 citation statements)

References 30 publications

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“…The steady continuation of atoh8 expression in the second phase supports our assumption of atoh8 marking the sclerotome in the somite. The areas associated with atoh8 expression have been shown to be positive for other markers such as twist (Germanguz et al 2007 ; Gitelman 2007 ; Yeo et al, 2007 , 2009 ), pax (Liu et al 2020 ; Ma et al 2018 ; Nornes et al 1996 ) and nkx3 (Ma et al 2018 ), all genes which are known to be specifically expressed in the sclerotome of vertebrates. Our expression profile of atoh8 furthermore corroborates a previously observed finding (Ma et al 2018 ), which points to a novelty regarding the established location of the vertebrate sclerotome: while in amniotes the sclerotome is restricted to a ventral domain from which later on in development cells migrate along the notochord to dorsal positions, there exists a further, dorsal domain in zebrafish (Ma et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

atoh8 expression pattern in early zebrafish embryonic development

Fragale

Divvela

Brand‐Saberi

2021

Histochem Cell Biol

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Atonal homologue 8 (atoh8) is a basic helix-loop-helix transcription factor expressed in a variety of embryonic tissues. While several studies have implicated atoh8 in various developmental pathways in other species, its role in zebrafish development remains uncertain. So far, no studies have dealt with an in-depth in situ analysis of the tissue distribution of atoh8 in embryonic zebrafish. We set out to pinpoint the exact location of atoh8 expression in a detailed spatio-temporal analysis in zebrafish during the first 24 h of development (hpf). To our surprise, we observed transcription from pre-segmentation stages in the paraxial mesoderm and during the segmentation stages in the somitic sclerotome and not—as previously reported—in the myotome. With progressing maturation of the somites, the restriction of atoh8 to the sclerotomal compartment became evident. Double in situ hybridisation with atoh8 and myoD revealed that both genes are expressed in the somites at coinciding developmental stages; however, their domains do not spatially overlap. A second domain of atoh8 expression emerged in the embryonic brain in the developing cerebellum and hindbrain. Here, we observed a specific expression pattern which was again in contrast to the previously published suggestion of atoh8 transcription in neural crest cells. Our findings point towards a possible role of atoh8 in sclerotome, cerebellum and hindbrain development. More importantly, the results of this expression analysis provide new insights into early sclerotome development in zebrafish—a field of research in developmental biology which has not received much attention so far.

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Section: Discussionmentioning

confidence: 99%

atoh8 expression pattern in early zebrafish embryonic development

Fragale

Divvela

Brand‐Saberi

2021

Histochem Cell Biol

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“…In Xenopus embryos, Pax1 transcripts starts to be detected in early somitogenesis (st. 17) and becomes more and more abundant (st. 20–45) in the sclerotome and endodermal pharyngeal pouches, as reported for other vertebrates ( Sanchez and Sanchez, 2013 ). Zebrafish has two pax1 paralogues (pax1a and pax1b), both of which exhibit expression in the developing pharyngeal pouches, and sclerotomes from 18 to 96 hpf ( Liu Y. H. et al, 2020 ).…”

Section: Pax1 Displays Conserved Expression Patterns During Vertebrat...mentioning

confidence: 99%

An overview of PAX1: Expression, function and regulation in development and diseases

Kong²,

Jia³

et al. 2022

Front. Cell Dev. Biol.

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Transcription factors play multifaceted roles in embryonic development and diseases. PAX1, a paired-box transcription factor, has been elucidated to play key roles in multiple tissues during embryonic development by extensive studies. Recently, an emerging role of PAX1 in cancers was clarified. Herein, we summarize the expression and functions of PAX1 in skeletal system and thymus development, as well as cancer biology and outline its cellular and molecular modes of action and the association of PAX1 mutation or dysregulation with human diseases, thus providing insights for the molecular basis of congenital diseases and cancers.

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“…Tbx1 regulates pouch morphogenesis by controlling Wnt11r and Fgf8a expression in the head mesoderm in zebrafish ( Choe & Crump, 2014 ). Pax1 promotes pouch formation by expressing tbx1 and fgf3 in the PE in medaka and zebrafish, with Foxi1 controlling pouch development through ectodermal Wnt4a expression in zebrafish ( Okada et al, 2016 ; Jin et al, 2018 ; Liu et al, 2020 ). The cellular process of pouch formation has been studied in detail in zebrafish.…”

Section: Introductionmentioning

confidence: 99%

“…Tbx1 is a master regulator for developing pouches in fish to human, and TBX1 haploinsufficiency is the genetic cause of DGS ( Lindsay et al, 2001 ; Piotrowski et al, 2003 ; Tran et al, 2011 ). Pax1/9 and Foxi1/3 are required for pouch formation in fish and mice ( Peters et al, 1998 ; Nissen et al, 2003 ; Solomon et al, 2003 ; Edlund et al, 2014 ; Okada et al, 2016 ; Jin et al, 2018 ; Liu et al, 2020 ). Signaling molecules such as Fgf3/8, Wnt11r/4a, EphrinB2/B3, and Bmp are also necessary for pouch development in either zebrafish or mice ( Crump et al., 2004 ; Herzog et al, 2004 ; Agrawal et al, 2012 ; Choe et al, 2013 ; Choe & Crump, 2015 ; Lovely et al, 2016 ; Li et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Expression and Functional Analysis of cofilin1-like in Craniofacial Development in Zebrafish

Jin¹,

Jeon²,

Choe³

2022

Dev. Reprod.

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Pharyngeal pouches, a series of outgrowths of the pharyngeal endoderm, are a key epithelial structure governing facial skeleton development in vertebrates. Pouch formation is achieved through collective cell migration and rearrangement of pouch-forming cells controlled by actin cytoskeleton dynamics. While essential transcription factors and signaling molecules have been identified in pouch formation, regulators of actin cytoskeleton dynamics have not been reported yet in any vertebrates. Cofilin1-like (Cfl1l) is a fish-specific member of the Actin-depolymerizing factor (ADF)/Cofilin family, a critical regulator of actin cytoskeleton dynamics in eukaryotic cells. Here, we report the expression and function of cfl1l in pouch development in zebrafish. We first showed that fish cfl1l might be an ortholog of vertebrate adf , based on phylogenetic analysis of vertebrate adf and cfl genes. During pouch formation, cfl1l was expressed sequentially in the developing pouches but not in the posterior cell mass in which future pouch-forming cells are present. However, pouches, as well as facial cartilages whose development is dependent upon pouch formation, were unaffected by loss-of-function mutations in cfl1l . Although it could not be completely ruled out a possibility of a genetic redundancy of Cfl1l with other Cfls, our results suggest that the cfl1l expression in the developing pouches might be dispensable for regulating actin cytoskeleton dynamics in pouch-forming cells.

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Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development

Cited by 15 publications

References 30 publications

atoh8 expression pattern in early zebrafish embryonic development

atoh8 expression pattern in early zebrafish embryonic development

An overview of PAX1: Expression, function and regulation in development and diseases

Expression and Functional Analysis of cofilin1-like in Craniofacial Development in Zebrafish

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