Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion

Shen, Wenyi; Pu, Juan; Sun, Jing; Tan, Bing; Wang, Wei; Wang, Lili; Cheng, Jianmeng; Zuo, Yangsong

doi:10.1186/s12935-020-01460-z

Cited by 29 publications

(18 citation statements)

References 37 publications

(64 reference statements)

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“… [ 135 ] Lung cancer Xenograft model In the zebrafish xenograft model, knockdown of LINC00152 reduced the proliferation and migration of lung cancer cells and enhanced the inhibition effect of afatinib for lung cancer progression in cultured cells and the zebrafish xenograft model. [ 69 ] Lung cancer Xenograft model BPIQ-induced anti-lung cancer is involved in mitochondrial apoptosis. BPIQ could be a promising anti-lung cancer drug for further applications.…”

Section: Zebrafish Modelmentioning

confidence: 99%

“…In order to improve the diagnosis and treatment of lung cancer, it is necessary to further understand the pathogenesis and related molecular mechanism of lung cancer. Shen et al 69 established a xenotransplantation model after LINC00152 knockout lung cancer cells were implanted into zebrafish. The comparison between the control group and stereoscopic microscope showed that the silencing of LINC00152 could inhibit the proliferation and metastasis of lung cancer cells.…”

Section: Zebrafish Modelmentioning

confidence: 99%

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Application of Animal Models in Cancer Research: Recent Progress and Future Prospects

Wen-ling

Wang

et al. 2021

CMAR

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Animal models refers to the animal experimental objects and related materials that can simulate human body established in medical research. As the second-largest disease in terms of morbidity and mortality after cardiovascular disease, cancer has always been the focus of human attention all over the world, which makes it a research hotspot in the medical field. At the same time, more and more animal models have been constructed and used in cancer research. With the deepening of research, the construction methods of cancer animal models are becoming more and more diverse, including chemical induction, xenotransplantation, gene programming, and so on. In recent years, patient-derived xenotransplantation (PDX) model has become a research hotspot because it can retain the microenvironment of the primary tumor and the basic characteristics of cells. Animal models can be used not only to study the biochemical and physiological processes of the occurrence and development of cancer in objects but also for the screening of cancer drugs and the exploration of gene therapy. In this paper, several main tumor animal models and the application progress of animal models in tumor research are systematically reviewed. Finally, combined with the latest progress and development trend in this field, the future research of tumor animal model was prospected.

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Section: Zebrafish Modelmentioning

confidence: 99%

Section: Zebrafish Modelmentioning

confidence: 99%

Application of Animal Models in Cancer Research: Recent Progress and Future Prospects

Wen-ling

Wang

et al. 2021

CMAR

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“…The similarities in the immune cells, cytokines, and the presence of various receptors that are required for host-pathogen interactions makes the correlation of zebrafish research to humans, very much relevant ( Targen et al, 2020 ). Xeno-transplantation was achieved by introducing human lung epithelial A549 cells into the swim bladder of the fish ( Shen et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Giloy Ghanvati (Tinospora cordifolia (Willd.) Hook. f. and Thomson) Reversed SARS-CoV-2 Viral Spike-Protein Induced Disease Phenotype in the Xenotransplant Model of Humanized Zebrafish

2021

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The current Severe Acute Respiratory Syndrome disease caused by Coronavirus-2 (SARS-CoV-2) has been a serious strain on the healthcare infrastructure mainly due to the lack of a reliable treatment option. Alternate therapies aimed at symptomatic relief are currently prescribed along with artificial ventilation to relieve distress. Traditional medicine in the form of Ayurveda has been used since ancient times as a holistic treatment option rather than targeted therapy. The practice of Ayurveda has several potent herbal alternatives for chronic cough, inflammation, and respiratory distress which are often seen in the SARS-CoV-2 infection. In this study we have used the aqueous extracts of Tinospora cordifolia (willd.) Hook. f. and Thomson in the form of Giloy Ghanvati, as a means of treatment to the SARS-CoV-2 spike-protein induced disease phenotype in a humanized zebrafish model. The introduction of spike-protein in the swim bladder transplanted with human lung epithelial cells (A549), caused an infiltration of pro-inflammatory immune cells such as granulocytes and macrophages into the swim bladder. There was also an increased systemic damage as exemplified by renal tissue damage and increased behavioral fever in the disease induction group. These features were reversed in the treatment group, fed with three different dosages of Giloy Ghanvati. The resultant changes in the disease phenotype were comparable to the group that were given the reference compound, Dexamethasone. These findings correlated well with various phyto-compounds detected in the Giloy Ghanvati and their reported roles in the viral disease phenotype amelioration.

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“…Several recent studies have demonstrated the ceRNA mechanism of different lncRNAs in HCC metastasis, such as HULC/miR-372/PRKACB, LINC00974/miR-642/KRT19, AGAP2-AS1/miR-16-5p/ANXA11, and so on [19][20][21][22]. Zebra sh xenograft can offer the fast and intuitive metastasis model in cancer research for lncRNA study [23], which prompts us to explore its application in veri cation of ceRNA network in cancer metastasis.…”

Section: Introductionmentioning

confidence: 99%

LncRNA HOXD-AS1 functions as a ceRNA to promote hepatocellular carcinoma metastasis in zebrafish xenograft models

Zhang¹,

Wang²,

Feng³

et al. 2021

Preprint

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Background: A few studies have shown that long noncoding RNA (lncRNA) HOXD cluster antisense RNA 1 (HOXD-AS1) plays an important role in hepatocellular carcinoma (HCC) metastasis as a competing endogenous RNA (ceRNA), but there is little in vivo evidence. This study aims to explore the zebrafish HCC xenograft as an in vivo metastasis model to verify the ceRNA network of HOXD-AS1. Methods: The quantitative reverse transcription PCR (qRT-PCR) assay was used to assess the expression level of HOXD-AS1 in HCC cell lines. Knockdown of HOXD-AS1 or miR-130a-3p was performed by transfecting small interfering RNA (siRNA) or microRNA (miRNA) inhibitor, respectively. The proliferation and invasion of HCC cells in vitro were analyzed by CCK-8 and transwell assays. The growth and metastasis of HCC cells in vivo were assessed by zebrafish xenograft models.Results: We verified that HOXD-AS1 was overexpressed in all tested HCC cell lines than the normal hepatic cells. Silence of HOXD-AS1 suppressed cell proliferation and invasion in Hep3B and Huh7 HCC cell lines in vitro. In zebrafish xenograft models, knockdown of HOXD-AS1 also reduced the growth and metastasis of the two HCC cells. Moreover, downregulation of miR-130a-3p not only increased the HCC metastasis, but also rescued the metastasis which inhibited by silence of HOXD-AS1 in vitro and in vivo.Conclusions: Our study demonstrates the metastasis role of the HOXD-AS1/miR-130a-3p ceRNA network in HCC cells in vitro and in vivo, and these findings suggest that zebrafish xenograft model could be used for ceRNA mechanism verification in tumor metastasis.

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Zebrafish xenograft model of human lung cancer for studying the function of LINC00152 in cell proliferation and invasion

Cited by 29 publications

References 37 publications

Application of Animal Models in Cancer Research: Recent Progress and Future Prospects

Application of Animal Models in Cancer Research: Recent Progress and Future Prospects

Giloy Ghanvati (Tinospora cordifolia (Willd.) Hook. f. and Thomson) Reversed SARS-CoV-2 Viral Spike-Protein Induced Disease Phenotype in the Xenotransplant Model of Humanized Zebrafish

LncRNA HOXD-AS1 functions as a ceRNA to promote hepatocellular carcinoma metastasis in zebrafish xenograft models

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