Zein/Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) electrospun blend fiber scaffolds: Preparation, characterization and cytocompatibility

Cai, Zengxuan; Zhang, Qin; Xianyou, Song; Liu, Yuanpei

doi:10.1016/j.msec.2016.10.053

Cited by 34 publications

(20 citation statements)

References 56 publications

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“…Structural proteins are critical components of the extracellular matrix (ECM) that provides appropriate strength and toughness [ 13 , 14 ]. Moreover, two or more polymers are often blended to overcome the limitations [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells

et al. 2017

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Periodontitis is a widespread dental disease affecting 10 to 15% of worldwide adult population, yet the current treatments are far from satisfactory. The human periodontal ligament stem cell is a promising potential seed cell population type in cell-based therapy and tissue regeneration, which require appropriate scaffold to provide a mimic extracellular matrix. Zein, a native protein derived from corn, has an excellent biodegradability, and therefore becomes a hotspot on research and application in the field of biomaterials. However, the high hydrophobicity of zein is unfavorable for cell adhesion and thus greatly limits its use. In this study, we fabricate co-electrospun zein/gelatin fiber scaffolds in order to take full advantages of the two natural materials and electrospun fiber structure. Zein and gelatin in four groups of different mass ratios (100:00, 100:20, 100:34, 100:50), and dissolved the mixtures in 1,1,1,3,3,3-hexafluoro-2-propanol, then produced membranes by electrospinning. The results showed that the scaffolds were smooth and homogeneous, as shown in scanning electron micrographs. The diameter of hybrid fibers was increased from 69 ± 22 nm to 950 ± 356 nm, with the proportion of gelatin increase. The cell affinity of zein/gelatin nanofibers was evaluated by using human periodontal ligament stem cells. The data showed that hydrophilicity and cytocompatibility of zein nanofibers were improved by blended gelatin. Taken together, our results indicated that the zein/gelatin co-electrospun fibers had sufficient mechanical properties, satisfied cytocompatibility, and can be utilized as biological scaffolds in the field of tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells

et al. 2017

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“…Vigneswari et al (2016) prepared electrospun P(3HB-co-4HB)/collagen peptides for wound healing and reported that cell compatibility (in vitro) and wound healing (in vivo) were improved by adding collagen peptides to P(3HB-co-4HB). Zhijiang et al (2017) controlled mechanical properties of electrospun P(3HB-co-4HB) by blending zein, natural protein obtained from corn, and confirmed the electrospun P(3HB-co-4HB)/zein was biocompatibile in vitro. Nishizuka et al (2014) focused on the excellent elongation property of P(3HB-co-4HB) to develop a novel fracture fixation device, intramedullaryfixation with biodegradable materials, for weakened long bones using electrospun P(3HB-co-4HB) as a bone-cement bag.…”

mentioning

confidence: 66%

X-ray Tomographic Imaging of Tensile Deformation Modes of Electrospun Biodegradable Polyester Fibers

et al. 2017

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Electrospinning allows the production of fibrous networks for tissue engineering, drug delivery, and wound healing in health care. It enables the production of constructs with large surface area and a fibrous morphology that closely resembles the extracellular matrix of many tissues. A fibrous structure not only promotes cell attachment and tissue formation but could also lead to very interesting mechanical properties. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)) is a biodegradable polyester that exhibits a large (>400%) elongation before failure. In this study, synchrotron X-ray phase contrast imaging was performed during tensile deformation to failure on a non-woven fiber mat of P(3HB-co-4HB) fibers. Significant reorientation of the fibers in the straining direction was observed, followed by localized necking and eventual failure. From an original average fiber diameter of 4.3 µm, a bimodal distribution of fiber diameter (modal diameters of 1.9 and 3.7 µm) formed after tensile deformation. Extensive localized necking (thinning) of fibers between (thicker) fiber-fiber contacts was found to be the cause for non-uniform thinning of the fibers, a phenomenon that is expected but has not been observed in 3D previously. The data presented here have implications not only in tissue regeneration but for fibrous materials in general.

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“…Based on vitro assays, it was found that the blended scaffolds were noncytotoxic for NIH3T3 fibroblast cells and MG-63 osteoblast cells and supported cell attachment, distribution, and proliferation. The good physiochemical and biological properties of electrospun fibrous scaffolds make them attractive for use in tissue engineering applications [209].…”

Section: Plant Protein-based Green Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

Jahangirian

Azizi²,

Rafiee-Moghaddam

et al. 2019

Biomolecules

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In recent decades, regenerative medicine has merited substantial attention from scientific and research communities. One of the essential requirements for this new strategy in medicine is the production of biocompatible and biodegradable scaffolds with desirable geometric structures and mechanical properties. Despite such promise, it appears that regenerative medicine is the last field to embrace green, or environmentally-friendly, processes, as many traditional tissue engineering materials employ toxic solvents and polymers that are clearly not environmentally friendly. Scaffolds fabricated from plant proteins (for example, zein, soy protein, and wheat gluten), possess proper mechanical properties, remarkable biocompatibility and aqueous stability which make them appropriate green biomaterials for regenerative medicine applications. The use of plant-derived proteins in regenerative medicine has been especially inspired by green medicine, which is the use of environmentally friendly materials in medicine. In the current review paper, the literature is reviewed and summarized for the applicability of plant proteins as biopolymer materials for several green regenerative medicine and tissue engineering applications.

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Zein/Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) electrospun blend fiber scaffolds: Preparation, characterization and cytocompatibility

Cited by 34 publications

References 56 publications

Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells

Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells

X-ray Tomographic Imaging of Tensile Deformation Modes of Electrospun Biodegradable Polyester Fibers

Status of Plant Protein-Based Green Scaffolds for Regenerative Medicine Applications

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