Zell- und Gewebekultur

Gstraunthaler, Gerhard; Lindl, Toni

doi:10.1007/978-3-662-62606-1

Cited by 3 publications

(1 citation statement)

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“…Organ culture models serve as a promising alternative to animal models, as in vitro cultivation reduces the number of experimental animals. By preserving the tissue in its three-dimensional compsition, it is possible to analyse structural and synaptic organizations (Gstraunthaler und Lindl 2013; Humpel 2018). The viability of organotypic postnatal hippocampal cultures, as well as other organs, can be preserved over a limited period of time (Gogolla et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Methodological comparison of different approaches to cultivate postnatal organotypic hippocampal cultures

Czapla

Reiterer

Funke

2022

Preprint

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To study molecular and cellular processes of the brain in vitro, the cultivation of postnatal organotypic hippocampal slices should be established. The methodological approach had to be optimized several times because the result of the cultivation was not optimal. Initially, cultivation was performed by roller tube culture, which is the first developed cultivation method for these cultures. As the cultures detached or even dissolved, further cultivation was carried out using inserts, which represent a modification of the roller tube cultures. In the further course it came also to the use of different preparation methods and to the exchange of the medium and the incubator, in order to obtain a result of the organ cultivation corresponding to the expectations. The results show that cultivation on semi-permeable membranes has an advantage over roller tube cultures. On the one hand, there is no detachment and dissolution of hippocampal slices due to the mechanical influence and on the other hand, the tissue shows an improvement in general condition compared to roller tube cultures. Furthermore, it was found that an acceleration of the preparation time has positive effects on the neurons. In addition, it could be shown by the recorded time series that a cultivation over a longer period of time stabilizes the tissue. If the hippocampi remain connected to the entorhinal cortex, wallerian degeneration does not occur, resulting in longer survival of the cells in the postnatal cultures and thus strengthening the tissue.

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Section: Introductionmentioning

confidence: 99%

Methodological comparison of different approaches to cultivate postnatal organotypic hippocampal cultures

Czapla

Reiterer

Funke

2022

Preprint

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Mikrophysiometrischer Test zur Evaluierung serumfreier Zellkulturmedien

Eggert¹,

Stetsenko²,

Wiest³

2022

Biospektrum

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Nitrogen Accumulation in Oyster (Crassostrea gigas) Slurry Exposed to Virucidal Cold Atmospheric Plasma Treatment

Csadek

Paulsen

Weidinger

et al. 2021

Life

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Viral contamination of edible bivalves is a major food safety issue. We studied the virucidal effect of a cold atmospheric plasma (CAP) source on two virologically different surrogate viruses [a double-stranded DNA virus (Equid alphaherpesvirus 1, EHV-1), and a single-stranded RNA virus (Bovine coronavirus, BCoV)] suspended in Dulbecco’s Modified Eagle’s Medium (DMEM). A 15 min exposure effectuated a statistically significant immediate reduction in intact BCoV viruses by 2.8 (ozone-dominated plasma, “low power”) or 2.3 log cycles (nitrate-dominated, “high power”) of the initial viral load. The immediate effect of CAP on EHV-1 was less pronounced, with “low power” CAP yielding a 1.4 and “high power” a 1.0 log reduction. We observed a decline in glucose contents in DMEM, which was most probably caused by a Maillard reaction with the amino acids in DMEM. With respect to the application of the virucidal CAP treatment in oyster production, we investigated whether salt water could be sanitized. CAP treatment entailed a significant decline in pH, below the limits acceptable for holding oysters. In oyster slurry (a surrogate for live oysters), CAP exposure resulted in an increase in total nitrogen, and, to a lower extent, in nitrate and nitrite; this was most probably caused by absorption of nitrate from the plasma gas cloud. We could not observe a change in colour, indicative for binding of NOx to haemocyanin, although this would be a reasonable assumption. Further studies are necessary to explore in which form this additional nitrogen is deposited in oyster flesh.

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Zell- und Gewebekultur

Cited by 3 publications

References 0 publications

Methodological comparison of different approaches to cultivate postnatal organotypic hippocampal cultures

Methodological comparison of different approaches to cultivate postnatal organotypic hippocampal cultures

Mikrophysiometrischer Test zur Evaluierung serumfreier Zellkulturmedien

Nitrogen Accumulation in Oyster (Crassostrea gigas) Slurry Exposed to Virucidal Cold Atmospheric Plasma Treatment

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