Zellkern-DNS-Analysen durch Einzelzell-Fluoreszenz-Zytophotometrie an Prostatakarzinomen vor und während der Therapie

Seppelt, U; Sprenger, E

doi:10.1007/978-3-642-81706-9_13

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…The few publications available on analyses of prostatic carcino mas by means of single-cell scanning cytophotometry and flow-through cytophotometry differ in their results; they do report high DNA values especially for dediffer entiated carcinoma, though [12,13,16].…”

Section: Discussionmentioning

confidence: 99%

Nuclear DNA Analysis: The Relevance of Ploidy, DNA Heterogeneity and Phases of the Cell Cycle in 329 Patients with Prostatic Carcinoma

Al-Abadi

Nagel²

1990

Urol Int

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The ploidy, DNA heterogeneity and the phases of the cell cycle of the tumor were analyzed, by means of single-cell DNA cytophotometry, in 329 patients with locally advanced prostatic carcinoma to find out and establish prognostic factors apart from those already known (stage, grade). Follow-up periods ranged from 1 to 9 years. 253 (76.8%) of the 329 patients had carcinoma stage T₃ N_x M₀, and 76 of them (23.1 %) had carcinoma stage T₃/T₄ N₂_––4 M₁. 11.8% of the patients showed a cytological grade of malignancy I, while 64.3% had grade II carcinoma and 23.8% had grade III carcinoma. Single-cell DNA cytophotometry demonstrated aneuploidy rates of up to 71 % and diploidy rates of up to 23.8% for the higher grades of malignancy, i.e. grades II and III, whereas the diploidy rate established for grade I was 68% and the respective aneuploidy rate was 21%. These differences are significant (p < 0.001). There was a significant correlation between the results of DNA cytophotometry and the clinical course of the disease. Only 3 (3.7%) of the patients with diploid tumor cell nuclei developed metastases and local tumor progression within 8 years, whereas patients with aneuploid tumor cell nuclei showed metastases and local tumor progression within 8–22 months. These patients died of carcinoma after an average 18 months following primary diagnosis.

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Section: Discussionmentioning

confidence: 99%

Nuclear DNA Analysis: The Relevance of Ploidy, DNA Heterogeneity and Phases of the Cell Cycle in 329 Patients with Prostatic Carcinoma

Al-Abadi

Nagel²

1990

Urol Int

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“…Die vorliegenden Ergebnisse der DNS-Untersuchungen deuten auf ein unterschiedliches Wachstumsverhalten des Prostatakarzinoms und damit auf seine Heterogenität hin. Die wenigen bisher vorliegenden Untersuchungen an Prostatakarzinomen mittels der Einzelzellzytophotometrie als auch mit der Durchflußzytophotometrie zeigen unterschiedliche Ergebnisse und berichten über hohe DNS-Werte, besonders bei entdifferenzierten Karzinomen (12,13,16).…”

Section: Diskussionunclassified

Prognostische Bedeutung von Ploidie und proliferativer Aktivität beim lokal fortgeschrittenen Prostatakarzinom

Al-Abadi¹,

Nagel²

1988

Aktuel Urol

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“…As the histological grading is subjective and nonuniform, it is necessary to assess the grade of malignancy objectively by means of prognostic methods of investigation. Single-cell DNA cytometry and flow-through DNA cytometry are such methods [ 1,2,4,6,7,9,10,15,20,21,23,24], DNA cytophotometry of the actual tumor cells is the only method which per mits more accurate assessment of differentiated malig nancy potentials in tumor cell nuclei and prediction of tumor progression than is possible with visual morphol ogy alone [1-3, 20, 22, 26]. In the present prospective study ploidy, DNA heterogeneity and the counts of cell cycle phases in the individual malignancy grades were investigated by single-cell DNA absorption cytophotom etry with the aim of finding new cellular prognostic fac tors which could be used in addition to those conven tionally employed (stage and grade).…”

Section: Introductionmentioning

confidence: 99%

Prognostic Relevance of DNA Ploidy and Proliferative Activity in Urothelial Carcinoma of the Renal Pelvis and Ureter

Borgmann

Al-Abadi²,

Nagel³

1991

Urol Int

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In 55 patients with urothelial carcinoma of the renal pelvis or ureter, the ploidy, the DNA heterogeneity and the counts of cell cycle phases in the tumor were examined by means of single-cell DNA cytophotometry in order to find more prognostic factors than those already known (stage and grade). Follow-up periods ranged from 1 to 6 years. At the time of first diagnosis, 42 (76%) of the patients had tumors of the renal pelvis, 13 (24%) of them had ureteral tumors. 23 (42%) patients were in stage pT 1 N 0, 15 (27%) in stage pT 2 N 0, 12 (22%) in stage pT 3 N 0, and 5 (9%) were in stage pT 3 N +. The histological malignancy grade most frequently seen in the patients examined – i.e. in 51% of cases – was malignancy grade II. 25% of the patients had grade III tumors whereas only 24% had grade I tumors. With malignancy grade I, DNA cytophotometry showed DNA frequency peaks to be in the diploid range while tumors with malignancy grade II showed heterogenous DNA patterns. 71% of the patients with malignancy grade III showed aneuploid DNA values; 29% of them had polyploid DNA values. For malignancy grades II and III, the proliferation rate of the tumor cells was statistically significantly higher than for malignancy grade I. The determination of tumor heterogeneity and tumor cell proliferation by means of DNA cytophotometry gives valuable clues regarding prognosis.

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Zellkern-DNS-Analysen durch Einzelzell-Fluoreszenz-Zytophotometrie an Prostatakarzinomen vor und während der Therapie

Cited by 5 publications

References 6 publications

Nuclear DNA Analysis: The Relevance of Ploidy, DNA Heterogeneity and Phases of the Cell Cycle in 329 Patients with Prostatic Carcinoma

Nuclear DNA Analysis: The Relevance of Ploidy, DNA Heterogeneity and Phases of the Cell Cycle in 329 Patients with Prostatic Carcinoma

Prognostische Bedeutung von Ploidie und proliferativer Aktivität beim lokal fortgeschrittenen Prostatakarzinom

Prognostic Relevance of DNA Ploidy and Proliferative Activity in Urothelial Carcinoma of the Renal Pelvis and Ureter

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