Zeolite‐Coated Porous Arrays: A Novel Strategy for Enzyme Encapsulation

Marthala, V. R. Reddy; Friedrich, M; Zhou, Zhou; Distaso, Monica; Reuß, S.; Al-Thabaiti, Shaeel A.; Schwieger, Wilhelm; Hartmann, Martin

doi:10.1002/adfm.201404335

Cited by 15 publications

(5 citation statements)

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“…The method is based on the aerosol-assisted [40][41][42] assembly of TS-1 nanocrystals to form hollow zeolite microspheres with a hierarchical porosity and high epoxidation activity in water-based media. Hierarchical zeolites [43][44][45][46][47][48][49][50] have already been identied as possible candidates for enzyme immobilization 51,52 but never exploited in chemo-enzymatic catalysis. The presented aerosol method allows preparing hierarchical hollow zeolite structures using an easy, continuous, and scalable aerosol process.…”

Section: Rscli/chemical-science Introductionmentioning

confidence: 99%

Hollow zeolite microspheres as a nest for enzymes: a new route to hybrid heterogeneous catalysts

et al. 2020

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Section: Rscli/chemical-science Introductionmentioning

confidence: 99%

Hollow zeolite microspheres as a nest for enzymes: a new route to hybrid heterogeneous catalysts

et al. 2020

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“…Hierarchical zeolite [41][42][43][44][45][46][47][48] have already been identified as possible candidates for enzyme immobilization [49][50][51] but never exploited in chemo-enzymatic catalysis. Here, the presented aerosol method allows preparingin an easy, continuous, and scalable way 52 hierarchical zeolite structures with a central cavity that can accommodate large amounts of enzyme.…”

Section: Introductionmentioning

confidence: 99%

“…The method is based on the aerosol-assisted 40 assembly of TS-1 nanocrystals to form hollow zeolite microspheres with a hierarchical porosity and high epoxidation activity in water-based media. Hierarchical zeolite [41][42][43][44][45][46][47][48] have already been identified as possible candidates for enzyme immobilization [49][50][51] but never exploited in chemo-enzymatic catalysis. Here, the presented aerosol method allows preparingin an easy, continuous, and scalable way 52 hierarchical zeolite structures with a central cavity that can accommodate large amounts of enzyme.…”

Section: Introductionmentioning

confidence: 99%

Hollow zeolite microspheres as a nest for enzymes: a new route to hybrid heterogeneous catalysts for chemo-enzymatic cascade reactions

Smeets¹,

Baaziz²,

Ersen³

et al. 2019

Preprint

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<div> <p>Integrating enzymatic and heterogeneous catalysis can pave the way to new and performing cascade chemical processes. In this perspective, the preparation of bifunctional structures combining both an active inorganic catalyst and an enzyme is a key. However, such combinations are not straightforward, for example in the case of zeolite catalysts for which enzyme immobilization would be restricted to the external surface. We overcame this challenge by developing a new kind of hybrid catalysts based on hollow zeolite microspheres. The method leverages on the aerosol-assisted assembly of TS-1 nanocrystals to form hollow zeolite microspheres with tailored hierarchical texture and high epoxidation activity in water. The latter spheres were subsequently loaded with glucose oxidase enzymes which were then cross-linked to secure their entrapment. This controlled design allows to combine all the decisive features of the zeolite with a high enzyme loading. A chemo-enzymatic reaction is demonstrated, where the structured zeolite microsphere is used both as a nest for the enzyme and as an efficient inorganic heterogeneous catalyst. The enzyme ensures the <i>in situ</i> production of H<sub>2</sub>O<sub>2</sub> subsequently utilized by the zeolite for the epoxidation of allylic alcohol. We anticipate our method will open up new perspectives in the field of hybrid catalysis. Starting from various catalytic nano-building blocks, hollow microspheres with open entry ways could be prepared using the aerosol process and could be used as vessels for enzymes or even multi-enzymatic systems, thereby giving access to a multitude of new heterogeneous chemo-biocatalysts. <br></p> </div>

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“…The effective interfacial retention of proteins lays the foundation for various protein-involved applications, such as antifouling interfaces, therapy or tissue engineering devices, catalytic surfaces, and active interfaces of smart devices. [1][2][3][4] The layer-by-layer technique is easy to conduct and can be used to precisely control film structures down to the nanoscopic scale, [5][6][7][8][9][10] and it has been frequently used in the fabrication of protein-containing films. [11][12][13] Both covalent immobilization and non-covalent entrapment have been explored in the construction of protein-containing multilayers.…”

Section: Introductionmentioning

confidence: 99%

A functional protein retention and release multilayer with high stability

Nie

Zhang

2016

Nanoscale

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Effective and robust interfacial protein retention lies at the heart of the fabrication of protein-based functional interfaces, which is potentially applicable in catalysis, medical therapy, antifouling, and smart devices, but remains challenging due to the sensitive nature of proteins. This study reports a general protein retention strategy to spatial-temporally confine various types of proteins at interfacial regions. The proteins were preserved in mesoporous silica nanoparticles embedded in covalently woven multilayers. It is worth noting that the protein retention strategy effectively preserves the catalytic capabilities of the proteins, and the multilayer structure is robust enough to withstand the bubbling catalytic reactions and could be repeatedly used due to conservation of proteins. The spatiotemporal retention of proteins could be adjusted by varying the number of capping layers. Furthermore, we demonstrate that the protein-loaded interfacial layers could not only be used to construct catalytic-active interfaces, but also be integrated as the power-generating unit to propel a macroscopic floating device.

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Zeolite‐Coated Porous Arrays: A Novel Strategy for Enzyme Encapsulation

Cited by 15 publications

References 50 publications

Hollow zeolite microspheres as a nest for enzymes: a new route to hybrid heterogeneous catalysts

Hollow zeolite microspheres as a nest for enzymes: a new route to hybrid heterogeneous catalysts

Hollow zeolite microspheres as a nest for enzymes: a new route to hybrid heterogeneous catalysts for chemo-enzymatic cascade reactions

A functional protein retention and release multilayer with high stability

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