Zeptomole Detection of Streptavidin Using Carbon Paste Electrode and Square‐Wave Voltammetry

Abstract

We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination -analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS).

Square‐Wave Voltammetry: A Review on the Recent Progress

“…The authors have shown that the method is specific, while its sensitivity is comparable to the electrophoretic methods developed for avidin determination in the transgenic plants. In another work [132] the authors have reported on ultra-sensitive detection of streptoavidin (from Streptomyces avidinii) in a zeptomolar concentrations by using carbon paste electrode.…”

Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements

“…SWV is one of the most sensitive electrochemical methods for analysis of biomolecules [8,18,20,21]. To prevent interferences [22] and to enable analysis of very low volumes [20,23], voltammetric methods can be coupled with adsorptive transfer stripping technique.…”

“…To prevent interferences [22] and to enable analysis of very low volumes [20,23], voltammetric methods can be coupled with adsorptive transfer stripping technique. The main improvement is based on electrode removing and rinsing after accumulating of target molecules [22,24].…”

An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode

“…The carbon paste was housed in a Teflon body having a 2.5 mm diameter of active disk surface. The electrode surface was polished before each determination with a soft filter paper prior to measurement [43-45]. For smoothing and baseline correction [46], the software GPES 4.4 was employed.…”