Zero‐Background Helicase‐Dependent Amplification and Its Application to Reliable Assay of Telomerase Activity in Cancer Cell by Eliminating Primer–Dimer Artifacts

Chen, Feng; Zhang, Dexin; Zhang, Qing; Zuo, Xiaolei; Fan, Chunhai; Zhao, Yongxi

doi:10.1002/cbic.201500605

Cited by 14 publications

(15 citation statements)

References 47 publications

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“…Optical assays are the most widely employed strategy for the detection of telomerase activity 45-62. Organic luminescent materials (molecular beacon, AIEgens) and inorganic nanomaterials (quantum dot, gold nanoparticle) which may release optical signal are used to monitor the telomerase product in vitro as well as in situ .…”

Section: Assays For the Detection Of Telomerase Activity Using Isothementioning

confidence: 99%

Advances in the detection of telomerase activity using isothermal amplification

Zhang¹,

Lou²,

Xia³

2017

Theranostics

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Telomerase plays a significantly important role in keeping the telomere length of a chromosome. Telomerase overexpresses in nearly all tumor cells, suggesting that telomerase could be not only a promising biomarker but also a potential therapeutic target for cancers. Therefore, numerous efforts focusing on the detection of telomerase activity have been reported from polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP) assays to PCR-free assays such as isothermal amplification in recent decade. In this review, we highlight the strategies for the detection of telomerase activity using isothermal amplification and discuss some of the challenges in designing future telomerase assays as well.

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Section: Assays For the Detection Of Telomerase Activity Using Isothementioning

confidence: 99%

Advances in the detection of telomerase activity using isothermal amplification

Zhang¹,

Lou²,

Xia³

2017

Theranostics

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“…However, it requires thermocycling. Due to the rapid development of molecular biotechnology, a variety of isothermal amplification methods using specific enzymes have been developed, including restriction enzyme‐dependent amplification, [ 40,45,75,76 ] helicase‐dependent amplification (HDA), [ 44,46,77,78 ] and rolling circle amplification (RCA). [ 48–51 ]…”

Section: Biosensing Applications Based On High‐order Dna‐crnsmentioning

confidence: 99%

“…Compared with other DNA amplification‐based microRNA assays, the HDA module was much more sensitive and much faster, and could exhibit a broader dynamical range. [ 44 ] In addition, the HDA module could also be developed for the detection of HIV‐1 DNA, [ 46 ] telomerase, [ 77 ] transcription factor, [ 78 ] and pathogens. [ 57 ]…”

Section: Biosensing Applications Based On High‐order Dna‐crnsmentioning

confidence: 99%

DNA‐Based Chemical Reaction Networks for Biosensing Applications

Jiang

Yuan

et al. 2020

Advanced Intelligent Systems

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The nature of biosensing is a biochemical reaction. DNA‐based chemical reaction networks (DNA‐CRNs) as a powerful programming language for describing behaviors of chemical reactions have shown great potential in designs and applications of biosensors. Due to their programmability, modularity, and versatility of the DNA strand, the performance of different detection strategies can be improved mainly by the rational design of DNA‐CRNs. Herein, an overview of the fundamental theory and biosensing processes of DNA‐CRNs is provided. Various detection strategies of DNA‐CRNs are introduced, either in a simple low‐order reaction model or in a complicated high‐order reaction type, in combination with some typical cases for the different detection purposes. In addition, an overview of the recent development of DNA‐CRNs for monitoring the cell microenvironment is presented, which is of significance to uncover some specific cell behaviors and functions. Finally, the roles of DNA‐CRNs in the rational design of high‐performance biosensors are summarized by pointing out the remaining challenges that impede the precise biosensing using DNA‐CRNs in complicated biological environments.

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“…The sensitivity, speed, and robustness of HDA assays can be further improved by restriction endonuclease-mediated DNA helicase homing, macromolecular crowding agents, and optimization of reaction enzyme mix [74]. HDA assays are critically dependent on primer design, and are often vulnerable to primer-dimer amplicon contamination [75]. Amplification of the primer-dimers in place of the target nucleic acid leads to erroneous detection and remains one of the major concerns for HDA based assays.…”

Section: Helicase-dependent Amplification (Hda)mentioning

confidence: 99%

Amplification chemistries in clinical virology

Dunbar

Das

2019

Journal of Clinical Virology

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Molecular diagnostic methods have evolved and matured considerably over the last several decades and are constantly being evaluated and adopted by clinical laboratories for the identification of infectious pathogens. Advancement in other technologies such as fluorescence, electronics, instrumentation, automation, and sensors have made the overall diagnostic process more accurate, sensitive, and rapid. Nucleic acid based detection procedures, which rely on the fundamental principles of DNA replication have emerged as a popular and standard diagnostic method, and several commercial assays are currently available based on different nucleic acid amplification techniques. This review focuses on the major amplification chemistries that are used for developing commercial assays and discusses their application in the clinical virology laboratory. Polymerase chain reaction (PCR)The development of PCR by Kary Mullis in the 1980s revolutionized the detection of microbial pathogens in clinical specimens. PCR is a highly sensitive method that involves cyclical heating and cooling of the reaction mixture to facilitate amplification of the target DNA [3]. The method consists of three basic steps (denaturation, annealing, and extension) and the reaction mixture requires four primary components (template DNA, primers, nucleotides, and a DNA polymerase) (Fig. 1a). Double-stranded target DNA (dsDNA) is thermally denatured to form a single-stranded DNA template (ssDNA). Oligonucleotide primers anneal to complementary target sequences on the nucleic acid template and are extended by the DNA polymerase, thus creating the resultant PCR product (amplicon). The reaction occurs in a programmable thermal https://doi.

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Zero‐Background Helicase‐Dependent Amplification and Its Application to Reliable Assay of Telomerase Activity in Cancer Cell by Eliminating Primer–Dimer Artifacts

Cited by 14 publications

References 47 publications

Advances in the detection of telomerase activity using isothermal amplification

Advances in the detection of telomerase activity using isothermal amplification

DNA‐Based Chemical Reaction Networks for Biosensing Applications

Amplification chemistries in clinical virology

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