In this paper, we define the inclusion graph Inc(A) of an S-act A which is a graph whose vertices are non-trivial subacts of A and two distinct vertices B 1 , B 2 are adjacent if B 1 ⊂ B 2 or B 2 ⊂ B 1 . We investigate the relationship between the algebraic properties of an S-act A and the properties of the graph Inc(A). Some properties of Inc(A) including girth, diameter and connectivity are studied. We characterize some classes of graphs which are the inclusion graphs of S-acts. Finally, some results concerning the domination number of such graphs are given.