Zerumbone induces growth inhibition of Burkitt’s lymphoma cell line via apoptosis

Abstract

Zerumbone (ZER), a natural compound has been extracted from Zingiber zerumbet with known pharmacological activities. The aim was to determine the anti-human Burkitt's lymphoma (Raji) cell effect of ZER. The 3-(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide (MTT) assay was used to determine cytotoxic effect while the Annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays was used to determine apoptotic effect of ZER on the human Burkitt's lymphoma (Raji) cell (ATCC CCL-86) cell line. The expressions of Bax, Bcl-2, and c-Myc genes were determined via real-time PCR. ZER suppressed the proliferation of Raji cells with a 48 h IC50 value of 5.1 μg/mL. Treated Raji cells also underwent late apoptosis especially after treatment with 100 μg/mL ZER. The apoptotic effect of ZER is associated with increase in Bax and decrease in Bcl-2 and c-Myc gene expressions. These results suggest that ZER inhibited the proliferation of Raji cells through the modification of apoptosis-related gene expressions. Therefore, ZER has potential as a candidate for the treatment of Burkitt's lymphoma.

The Anti-cancer Impact of Genetically Engineered Newcastle Disease Virus Expressing GFP Gene Against U87-MG Cell Line

“…The release of cytochrome C GFP was monitored using time-lapse imaging and fluorescence microscopy. These results also demonstrated that the extrinsic or intrinsic apoptosis pathway is activated by the release of cytochrome C-GFP, following the administration of apoptosis-inducing drugs [23,24].…”

Antiangiogenic Activity and Ros-Mediated Lung Cancer Cell Line Injury of Zerumbone

“…Among various anticancer mechanisms proposed, antiangiogenic and modulating intracellular ROS mechanism has also important role, in which immune systems are given opportunity to identify and respond to the tumor cells [14]. ZER, a natural sesquiterpene from edible ginger Zingiber zerumbet Smith, has anti-inflammatory, immunomodulatory, antibacterial [15][16][17], antioxidant, antinociceptive, and anti-proliferation activities against several cancer cells [18][19][20]. Even though, the pharmacological studies had shown the anticancer effects of ZER through the induction of apoptosis on human lung cancer cells [21], the antiangiogenic activity of ZER and its possible anticancer capacity by modulating intracellular ROS have not been clearly evidenced yet.…”