ZFP91: A Noncanonical NF-<i>κ</i>B Signaling Pathway Regulator with Oncogenic Properties Is Overexpressed in Prostate Cancer

Paschke, Lukasz; Jopek, Karol; Szyszka, Marta; Tyczewska, Marianna; Ziółkowska, Agnieszka; Ruciński, Marcin; Malendowicz, Ludwik K.

doi:10.1155/2016/6963582

Cited by 16 publications

(25 citation statements)

References 37 publications

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“…Among studied cancer types only in the case of prostate cancer ZFP91 expression was significantly elevated in comparison to the unchanged prostatic tissue. Similarly as in our previous study on this subject (15), the increased (even 10-fold) ZFP91 mRNA quantity was present only in the fraction of cancer cases. ZFP91 overexpression on mRNA level was observed by Lee et al (3) in cancers of prostate, stomach and liver.…”

Section: Discussionsupporting

confidence: 87%

“…Changes in ZFP91 mRNA levels observed in corresponding tissues were not reflected on a protein level. A discrepancy between mRNA and protein levels is often encountered (36)(37)(38)(39) and in the case of ZFP91 has been described in our previous works (14,15) as well as observed by the team of Lee et al (3). At this point of knowledge it seems that no clear correlation between ZFP91 mRNA and protein levels exist.…”

Section: Discussionsupporting

confidence: 56%

“…In prostate cancer cell lines ZFP91 protein was markedly upregulated compared to normal prostate epithelial cells (14). In most recent work ZFP91 mRNA was found to be significantly overexpressed in prostate cancer specimens (15).…”

Section: Introductionmentioning

confidence: 92%

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ZFP91 zinc finger protein expression pattern in normal tissues and cancers

et al. 2019

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Zinc finger protein 91 (ZFP91) gene has been recently acknowledged to possess oncogenic properties. To date, its expression has been examined only in a handful of human organs and cancer types. The aim of the present study was to characterize, for the first time, the ZFP91 expression pattern in a range of human tissues and cancer types. ZFP91 mRNA expression was examined using Cancer Survey cDNA sets. Utilized cDNA samples represented 15 human organs and 17 cancer types. ZFP91 mRNA expression was the highest in the testes and lymph nodes. It was downregulated in testis cancer, lymphoma and thyroid cancer, and upregulated in prostate cancer. Among the analyzed cancer types, ZFP91 expression was markedly elevated in sarcomas and melanoma. On a protein level, a large-scale reverse phase protein array was employed providing samples from 11 organ types and from cancers derived from these organs. ZFP91 protein expression was revealed to be generally stable across the tested samples and was only moderately elevated in breast, ovarian and pancreatic cancers. To the best of our knowledge, this is the first study to thoroughly analyze the ZFP91 expression pattern in human tissues and cancers. The obtained results provide the foundation for further work aiming to reveal its full biological significance.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 56%

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ZFP91 zinc finger protein expression pattern in normal tissues and cancers

et al. 2019

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“…Numerous studies have reported that zinc finger protein 91 (ZFP91) promotes proliferation and tumorigenesis of different cancer types via regulation of the NF-κB p65 pathway (12)(13)(14). Therefore, to further investigate the regulatory mechanism of the miR-188-5p/ZFP91 axis, we detected the expression of NF-κBp65 and RelB in BC cells.…”

Section: Mir-188-5p/zfp91 Axis Regulates Nf-kb/p65 and Relb Expressionmentioning

confidence: 99%

MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91

et al. 2020

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Breast cancer (BC) is the most commonly diagnosed malignant cancer in women. BC is the main cause of cancer-related death in women and seriously threatens the life and health of women worldwide. MicroRNAs (miRNAs/miRs) have been reported to regulate the development and progression of different types of cancer. However, the regulatory functions of miR-188-5p in BC have not been thoroughly demonstrated. In this present research, we identified that miR-188-5p was downregulated in BC tissues and several BC cell lines. Downregulation of miR-188-5p was significantly associated with advanced TNM stage. Moreover, we identified that miR-188-5p mimics significantly inhibited proliferation using CCK-8 assay, colony formation and xenograft animal model, suppressed invasion and migration detected by Transwell invasion assay, and increased the cellular apoptosis of BC cells as determined by cell apoptosis assay. Moreover miR-188-5p mimics also reduced the expression of NF-κB p65(Rel). To further investigate its regulatory mechanism, transcription factor zinc finger protein 91 (ZFP91) was predicted as the targeted protein of miR-188-5p by bioinformatic method. We confirmed their specific binding by dual luciferase (DLR) assay. We demonstrated that the overexpression of miR-188-5p significantly inhibited the expression of ZFP91 in BC cell lines and reduced the expression of NF-κB p65(Rel). An inverse correlation was found between the expression of miR-188-5p and ZFP91 in BC tissues. Importantly, we demonstrated that the restoration of ZFP91 was able to block the effect of miR-188-5p on the progression of MDA-MB-231 cells. Therefore, our study showed that miR-188-5p may be one of the important indicators and could inhibit the progression of human BC via targeting the ZFP91/NF-κB p65(Rel) signaling pathway, suggesting that miR-188-5p may be a promising future target for BC treatment.

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“…Prostate cancer (PC) is the second most common cancer and the fifth leading cause of cancer deaths among men worldwide. A million new PC cases are diagnosed every year (Paschke et al, ). Historically, the incidence of PC has been lower in men from Asian countries than those from western countries (Lassed et al, ).…”

Section: Introductionmentioning

confidence: 99%

AMD3100 inhibits epithelial–mesenchymal transition, cell invasion, and metastasis in the liver and the lung through blocking the SDF‐1α/CXCR4 signaling pathway in prostate cancer

Zhu

Zhao

Zhou

2018

Journal Cellular Physiology

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Stromal cell‐derived factor‐1 (SDF‐1) and CXC chemokine receptor 4 (CXCR4) have been found to be tightly correlated with the progression of prostate cancer (PC). In this study, we investigated the effects of an SDF‐1α/CXCR4 inhibitor, AMD3100, on cell progression and metastasis potential of human PC cells. Human PC cell lines (LNCaP, PC3, and DU145) were cultured to detect SDF‐1α/CXCR4, which showed higher SDF‐1α and CXCR4 expression than the normal human prostate epithelial cell line, RWPE‐1. AMD3100 was confirmed to be an inhibitor of SDF‐1α, and to detect the effect of SDF‐1α/CXCR4 inhibition on PC, PC cells were treated with AMD3100 or/and CXCR4 siRNA. The results suggested that inhibition of the SDF‐1α/CXCR4 pathway could promote the E‐cadherin level but inhibit the levels of invasion and migration of vimentin, N‐cadherin and α5β1 integrin. Finally, tumor formation in nude mice was conducted, and the cell experiment results were verfied. These data show that AMD3100 suppresses epithelial–mesenchymal transition and migration of PC cells by inhibiting the SDF‐1α/CXCR4 signaling pathway, which provides a clinical target in the treatment of PC.

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ZFP91: A Noncanonical NF-κB Signaling Pathway Regulator with Oncogenic Properties Is Overexpressed in Prostate Cancer

Cited by 16 publications

References 37 publications

ZFP91 zinc finger protein expression pattern in normal tissues and cancers

ZFP91 zinc finger protein expression pattern in normal tissues and cancers

MicroRNA‑188‑5p inhibits the progression of breast cancer by targeting zinc finger protein 91

AMD3100 inhibits epithelial–mesenchymal transition, cell invasion, and metastasis in the liver and the lung through blocking the SDF‐1α/CXCR4 signaling pathway in prostate cancer

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