Zhenbao Pill reduces the percentage of Treg cells by inducing HSP27 expression

Zhenbao Pill reduces Treg cell proportion in acute spinal cord injury rats by regulating TUG1/miR-214/HSP27 axis

“…Further, Zhenbao Pill reduced the proportion of Treg cells by regulating the expression of HSP27 through TUG1/ miR-214 and thus improved ASCI. Compared with previous study [ 11 ], our study further elucidated the underlying molecular mechanism of how Zhenbao Pill reduced the proportion of Treg cells and improved ASCI. However, the TUG1/ miR-214 /HSP27 signaling pathway should be further confirmed in vivo in the future studies.…”

“…As reported, Zhenbao Pill could repair the damaged neurones, promote microcirculation, and remove oxygen free radicals [ 8 , 9 ], suggesting the potential application of Zhenbao Pill in treating SCI patients. Indeed, Zhenbao Pill has been shown to protect SCI rats from further damage [ 10 , 11 ], with improved BBB score. Consistent with this finding, in the present study, we also found that the BBB score was significantly lower in SCI group compared with sham group, which was dramatically reversed by Zhenbao Pill.…”

“…In an ASCI model, decrease in Treg cells were found to enhance the functional recovery following SCI, indicating that alleviated systemic immunosuppression could promote SCI repair [ 12 ]. Our previous study found that Zhenbao Pill inhibited the Treg lymphocyte amount by inhibiting the miR-214 induced expression of HSP27 during the repair of SCI [ 11 ]. This finding was also further confirmed by the present study: miR-214 mimic reversed the inhibition of Treg cell proportion and Foxp3 protein expression by Zhenbao Pill, which was abolished by the overexpression of HSP27.…”

“…Our previous research has found that Zhenbao Pill had a protective effect on the nerves of SCI rats [ 10 ]. We also found that Zhenbao Pill improved the performance of SCI rats in the Basso, Beattie, and Bresnahan (BBB) score and reduced the proportion of Treg cells [ 11 ].…”

“…Currently, it has been confirmed that the reduction in Treg cells and thus the alleviation of systemic immunosuppression can promote the repair of spinal cord injuries [ 12 ]. Our previous study shown that Zhenbao Pill inhibited the Treg lymphocyte numbers and the decrease in TGF-β levels by inhibiting the miR-214 induced expression of heat shock protein 27 (HSP27) during the repair of SCI [ 11 ]. However, studies are still required to further elucidate the underlying molecular mechanism of how Zhenbao Pill reduces the proportion of Treg cells and improves ASCI.…”