ZiBuPiYin recipe improves cognitive decline by regulating gut microbiota in Zucker diabetic fatty rats

Gu, Chunyan; Zhou, Wen; Wang, Wang; Xiang, Hong; Xu, Hui; Liang, Lina; Sui, Hua; Zhan, Lijun; Lu, Xiaoguang

doi:10.18632/oncotarget.14611

Cited by 23 publications

(11 citation statements)

References 56 publications

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“…Early reports have shown that ZBPYR improves the learning and memory process in high-fat diet combined with low-dose streptozotocin (STZ)-induced diabetic rats, and regulates the deposition of Ab in the brain (18). Our recent data also demonstrated that chronic psychological stress impairs glucose intolerance and decreases insulin sensitivity in Zucker diabetic fatty (ZDF) rats, suggesting that chronic psychological stress can contribute to the development of insulin resistance in T2DM (19). In the present work, we used spontaneous ZDF rats as a T2DM model and examined the hypothesis that ZBPYR could reverse the impairment of chronic psychological stress leading to DACD, mainly by reducing Ab deposition through the IRS-1/ AKT/mTOR signaling pathways in the medial prefrontal cortex (mPFC).…”

Section: Introductionmentioning

confidence: 63%

Effect of the ZiBuPiYin Recipe on Diabetes-Associated Cognitive Decline in Zucker Diabetic Fatty Rats After Chronic Psychological Stress

Zhan

Zhou

et al. 2020

Front. Psychiatry

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Background: Cognitive impairment is a complication of type 2 diabetes mellitus (T2DM) that affects the central nervous system (CNS). Studies have shown that chronic psychological stress may promote the development of T2DM into diabetes-associated cognitive decline (DACD). Previously, cognitive impairment in T2DM was correlated predominantly with insulin resistance in the medial prefrontal cortex (mPFC). Aims:We examined the effect of the ZiBuPiYin recipe (ZBPYR) on Zucker diabetic fatty (ZDF) rats and explored the impact of chronic stress on altered b-amyloid (Ab) metabolism through insulin receptor substrate (IRS) 1/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway after the induction of chronic psychological stress.Main Methods: After chronic psychological stress and drug treatment, cognitive function was observed via behavioral experiments. The activation of the hypothalamus-pituitaryadrenal (HPA) axis and levels of Ab were detected by enzyme-linked immunosorbent assay, and the expression of related proteins was evaluated by Western blotting.Key Findings: ZBPYR treatment significantly decreased anxious-like behaviors and plasma corticosterone (CORT) levels, and ameliorated learning and memory impairments of ZDF rats after chronic psychological stress. ZBPYR also reduced the deposition of Ab in the mPFC, improved brain insulin resistance, and modulated the mTOR-autophagy pathway.Significance: ZBPYR may be a potential therapeutic application for the treatment of DACD induced by chronic psychological stress.

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Section: Introductionmentioning

confidence: 63%

Effect of the ZiBuPiYin Recipe on Diabetes-Associated Cognitive Decline in Zucker Diabetic Fatty Rats After Chronic Psychological Stress

Zhan

Zhou

et al. 2020

Front. Psychiatry

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“…The progression of diabetes may be associated with microbiome dynamic changes; thus, we tracked the fecal microbiome changes in rats from 8 weeks of age until 15 weeks of age. Of note, we did not include rats older than 15 weeks of age in this work because published literature suggests that male ZDF rats exhibit significant diabetic complications at 15 weeks of age (Gu et al, 2017). A total of 1,944,426 16S rRNA (V3–V4 region) reads were obtained, averaging 60,763 reads per sample.…”

Section: Resultsmentioning

confidence: 99%

Dynamic Development of Fecal Microbiome During the Progression of Diabetes Mellitus in Zucker Diabetic Fatty Rats

Zhou

Zhan

et al. 2019

Front. Microbiol.

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Background: Although substantial efforts have been made to link the gut microbiota to type 2 diabetes, dynamic changes in the fecal microbiome under the pathological conditions of diabetes have not been investigated. Methods: Four male Zucker diabetic fatty (ZDF) rats received Purina 5008 chow [protein = 23.6%, Nitrogen-Free Extract (by difference) = 50.3%, fiber (crude) = 3.3%, ash = 6.1%, fat (ether extract) = 6.7%, and fat (acid hydrolysis) = 8.1%] for 8 weeks. A total of 32 stool samples were collected from weeks 8 to 15 in four rats. To decipher the microbial populations in these samples, we used a 16S rRNA gene sequencing approach. Results: Microbiome analysis showed that the changes in the fecal microbiome were associated with age and disease progression. In all the stages from 8 to 15 weeks, phyla Firmicutes , Bacteroidetes , Actinobacteria , and Proteobacteria primarily dominated the fecal microbiome of the rats. Although Lactobacillus and Turicibacter were the predominant genera in 8- to 10-week-old rats, Bifidobacterium , Lactobacillus , Ruminococcus , and Allobaculum were the most abundant genera in 15-week-old rats. Of interest, compared to the earlier weeks, relatively greater diversity (at the genus level) was observed at 10 weeks of age. Although the microbiome of 12-week-old rats had the highest diversity, the diversity in 13–15-week-old rats was reduced. Spearman’s correlation analysis showed that F/B was negatively correlated with age. Random blood glucose was negatively correlated with Lactobacillus and Turicibacter but positively correlated with Ruminococcus and Allobaculum and Simpson’s diversity index. Conclusion: We demonstrated the time-dependent alterations of the abundance and diversity of the fecal microbiome during the progression of diabetes in ZDF rats. At the genus level, dynamic changes were observed. We believe that this work will enhance our understanding of fecal microbiome development in ZDF rats and help to further analyze the role of the microbiome in metabolic diseases. Furthermore, our work may also provide an effective strategy for the clinical treatment of diabetes through microbial intervention.

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“…Coprococcus positively correlated with short-term object recognition memory and hippocampal PSD95 protein levels. A previous study showed that the abundance of Coprococcus was decreased in diabetic fatty rats with psychological-stress-induced diabetes-associated cognitive decline [ 51 ]. Erysipelatoclostridium positively correlated with long-term object recognition memory.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota from mice with cerebral ischemia-reperfusion injury affects the brain in healthy mice

Wang

Ren

et al. 2021

Aging

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Gut microorganisms can profoundly influence brain function in the host and their behavior. Since altered brain functional connectivity (FC) has been implicated in various cerebrovascular disorders, including cerebral ischemia-reperfusion (I/R) injury, we hypothesized that gut microbiota in mice with cerebral I/R injury would affect brain FC when transplanted into germ-free mice. Metagenomic analysis of germ-free male C57BL/6J mice colonized with microbiota from mice with and without cerebral I/R injury showed a clear distinction in microbiota composition between mice colonized with control and I/R microbiota. The I/R microbiota-colonized mice showed decreased FC in the cingulate cortex, hippocampus, and thalamus, and exhibited increased anxiety as well as diminished spatial learning and memory and short-term object recognition memory. I/R microbiota-colonized mice also had significantly reduced dendritic spine density and synaptic protein levels and exhibited increased hippocampal inflammation. These results indicate that gut microbiota components from mice with cerebral I/R injury can alter animal behavior, brain functional connectivity, hippocampal neuronal plasticity, and neuroinflammation. Moreover, they increase our understanding of the mechanisms through which the gut microbiome contributes to the pathobiology of cerebrovascular diseases.

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ZiBuPiYin recipe improves cognitive decline by regulating gut microbiota in Zucker diabetic fatty rats

Cited by 23 publications

References 56 publications

Effect of the ZiBuPiYin Recipe on Diabetes-Associated Cognitive Decline in Zucker Diabetic Fatty Rats After Chronic Psychological Stress

Effect of the ZiBuPiYin Recipe on Diabetes-Associated Cognitive Decline in Zucker Diabetic Fatty Rats After Chronic Psychological Stress

Dynamic Development of Fecal Microbiome During the Progression of Diabetes Mellitus in Zucker Diabetic Fatty Rats

Gut microbiota from mice with cerebral ischemia-reperfusion injury affects the brain in healthy mice

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