ZIF-8 labelled a new electrochemical aptasensor based on PEI-PrGO/AuNWs for DON detection

Ultrasensitive Electrochemiluminescence Biosensing Platform Based on Polymer Dots with Aggregation-Induced Emission for Dual-Biotoxin Assay

“…6,7 The European Union has issued the maximum residue levels of 0.75 mg•kg −1 for DON in cereals and the lethal oral dose of 0.1−1.0 μg•kg −1 for ABR in humans. 8,9 Unfortunately, DON and ABR usually exist in food, leading to synergistic and additive toxicological effects in the body. 10 In recent years, many analytical methods have been utilized for analyzing DON and ABR in foods, including high-performance liquid chromatography, 11 liquid chromatography-tandem mass spectrometry, 12 ultrahigh-performance liquid chromatography−mass spectrometry, 13 and electrochemical approaches.…”

“…At present, biotoxins show carcinogenic, teratogenic, neurotoxic, mutagenic, and immunotoxic effects, which threaten the world’s public health security. − Specifically, abrin (ABR) as a potential biological warfare pathogen and deoxynivalenol (DON) as a Group 3B carcinogen have attracted wide attention. , The European Union has issued the maximum residue levels of 0.75 mg·kg –1 for DON in cereals and the lethal oral dose of 0.1–1.0 μg·kg –1 for ABR in humans. , Unfortunately, DON and ABR usually exist in food, leading to synergistic and additive toxicological effects in the body . In recent years, many analytical methods have been utilized for analyzing DON and ABR in foods, including high-performance liquid chromatography, liquid chromatography-tandem mass spectrometry, ultrahigh-performance liquid chromatography–mass spectrometry, and electrochemical approaches. , In comparison, electrochemiluminescence (ECL), a highly efficient technology, has been considered as one of the most popular detection methods for biotoxin assay owing to its high sensitivity, low background, simple operation, and excellent controllability. − It should be noticed that the aptamers, short single oligonucleotide sequences, exhibit exceptional specificity and affinity for their corresponding targets through intermolecular interactions or electrostatic interactions, which makes them as valuable components for the design of ultrasensitive biosensors. , It is interesting that the ECL aptasensors from the combination of ECL and aptamers show ultrasensitivity and remarkable specificity toward molecular detection and open up great potential to develop more efficient and straightforward detection methods .…”