Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

Yang, Jiancheng; Carey, Patrick H.; Ren, F.; Mastro, Michael A.; Beers, Kimberly; Pearton, S. J.; Kravchenko, Ivan I.

doi:10.1063/1.5029902

Cited by 32 publications

(19 citation statements)

References 31 publications

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“…This strategy was successful enough to spot down to 47.8 aM of the target biomolecules [ 111 ]. The efficiency of these investigations and many others [ 89 , 91 , 92 , 93 , 103 , 106 , 107 , 108 , 110 , 115 ] acknowledge the capability of FET-based immunosensors in detecting infectious diseases.…”

Section: Surface Modification and Functionalization Of Chem/biofetsmentioning

confidence: 98%

Recent Advances of Field-Effect Transistor Technology for Infectious Diseases

et al. 2021

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Field-effect transistor (FET) biosensors have been intensively researched toward label-free biomolecule sensing for different disease screening applications. High sensitivity, incredible miniaturization capability, promising extremely low minimum limit of detection (LoD) at the molecular level, integration with complementary metal oxide semiconductor (CMOS) technology and last but not least label-free operation were amongst the predominant motives for highlighting these sensors in the biosensor community. Although there are various diseases targeted by FET sensors for detection, infectious diseases are still the most demanding sector that needs higher precision in detection and integration for the realization of the diagnosis at the point of care (PoC). The COVID-19 pandemic, nevertheless, was an example of the escalated situation in terms of worldwide desperate need for fast, specific and reliable home test PoC devices for the timely screening of huge numbers of people to restrict the disease from further spread. This need spawned a wave of innovative approaches for early detection of COVID-19 antibodies in human swab or blood amongst which the FET biosensing gained much more attention due to their extraordinary LoD down to femtomolar (fM) with the comparatively faster response time. As the FET sensors are promising novel PoC devices with application in early diagnosis of various diseases and especially infectious diseases, in this research, we have reviewed the recent progress on developing FET sensors for infectious diseases diagnosis accompanied with a thorough discussion on the structure of Chem/BioFET sensors and the readout circuitry for output signal processing. This approach would help engineers and biologists to gain enough knowledge to initiate their design for accelerated innovations in response to the need for more efficient management of infectious diseases like COVID-19.

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Section: Surface Modification and Functionalization Of Chem/biofetsmentioning

confidence: 98%

Recent Advances of Field-Effect Transistor Technology for Infectious Diseases

et al. 2021

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“…Since 2015, nine papers were published on AlGaN/ GaN HEMT for the detection of biomarkers of different diseases: bacterial infection [61], Zika virus [64], prostate cancer [65,69], heart failure [66], mental stress [68] and DNA [62].…”

Section: Minireviewmentioning

confidence: 99%

Electrochemical Affinity Sensors Using Field Effect Transducer Devices for Chemical Analysis

2021

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The review presents advances and main challenges of the affinity sensors based on field-effect transistors published during the last five years. The different nanomaterial-based field-effect transistors are classified according to the nature of the nanomaterials, beginning by silicon, the "gold-standard" semiconductor, the gallium nitride semiconductor, the organic semiconductors, the silicon nanowires, the inorganic nanomaterials, the carbon nanotubes and the graphene. Due to its exceptional electrical properties, the main works are devoted to graphene. The obtained analytical performances for the detection of biomarkers, of DNA sequences and of miRNA are listed. The relation between the operational conditions -nature of the nanomaterials, procedure of preparation, choice of the receptor molecule, method of immobilization -and the analytical performance are discussed. The perspective of industrialization of these affinity sensors based on field-effect transistors is discussed.

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“…24.1 shows the schematic of the sensor, consisting of an antibody-functionalized cover glass and an AlGaN/ GaN HEMT. Pulsed biasing of the electrode fabricated on the cover glass and functionalized with Zika antibody was used for detection [20]. The target Zika antigen (recombinant ZIKV NS1) solutions were diluted with bovine serum albumin (BSA) in pF 7.4 PBS solution with 0.1, 1, 10, or 100 ng/mL concentration.…”

Section: Zika Virusmentioning

confidence: 99%

“…The target Zika antigen (recombinant ZIKV NS1) solutions were diluted with bovine serum albumin (BSA) in pF 7.4 PBS solution with 0.1, 1, 10, or 100 ng/mL concentration. The reversible antigen and antibody binding through active sites on these two protein molecules and the HEMT drain current changes could fit with the Langmuir extension model [20]. In addition, the Hookean spring model was employed to simulate the relaxation portion of the time-dependent drain current.…”

Section: Zika Virusmentioning

confidence: 99%