Zika Virus: Quantification, Propagation, Detection, and Storage

Agbulos, Darrell; Barelli, Larissa; Giordano, Bryan V.; Hunter, Fiona F.

doi:10.1002/cpmc.19

Cited by 31 publications

(31 citation statements)

References 18 publications

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“…Nonetheless, to date, despite significant efforts to develop effective vaccines, no effective treatment for this emerging pathogen has been found. Therefore, identifying broad-spectrum antiviral drugs and effective clinical compounds is critical for the treatment of ZIKV patients and for the control of ZIKV outbreaks [25,26].…”

Section: Discussionmentioning

confidence: 99%

Cephalotaxine inhibits Zika infection by impeding viral replication and stability

Lai

2020

Biochemical and Biophysical Research Communications

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a b s t r a c tThe Zika virus (ZIKV) is a mosquito-borne flavivirus that has reemerged as a serious public health problem around the world. Syndromes of infected people range from asymptomatic infections to severe neurological disorders, such as Guillain-Barr e syndrome and microcephaly. Screening anti-ZIKV drugs derived from Chinese medicinal herbs is one method of identifying antiviral agents. In this paper, we report that (1) Cephalotaxine (CET), an alkaloid isolated from Cephalotaxus drupacea, was effective in inhibiting ZIKV activity in vitro (i.e., in Vero and A549 cell lines) and (2) the mechanisms which underlie these effects involve virucidal activity and a decrease in viral replication. Specifically, CET was found to decrease ZIKV RNA and viral protein expression, inhibit ZIKV replication, and inhibit ZIKV mRNA/protein production. We also determined that CET is effective in inhibiting dengue virus 1e4 (DENV1-4). Taken together, our findings indicate that CET could be an effective lead compound in the treatment of ZIKV and also suggest that further investigation and development of CET-derived drugs may lead to a new class of anti-Flavivirus medications.

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Section: Discussionmentioning

confidence: 99%

Cephalotaxine inhibits Zika infection by impeding viral replication and stability

Lai

2020

Biochemical and Biophysical Research Communications

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“…Plaque assays were conducted based on protocols provided by VIRAPUR and those developed by Agbulos et al ( 25 ) to determine the ZIKV stock viral titer prior to oral infections, with slight modifications. ZIKV stock titers were also assessed using qPCR (see ZIKV qPCR methods below).…”

Section: Methodsmentioning

confidence: 99%

Vertical transmission of Zika virus in orally-infected Aedes aegypti produces infectious adult progeny

Comeau

Zinna

Scott

et al. 2018

Preprint

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22Vertical transmission, or pathogen transfer from mother to offspring, can facilitate 23 persistence of emerging arboviruses, such as Zika virus (ZIKV), in mosquito populations. 24Understanding vertical transmission and the different environmental and temporal conditions that 25 affect it is important to assess whether new outbreaks could occur without reintroduction of the 26 virus. To determine the rate of vertical transmission for ZIKV, Aedes aegypti females were fed 27 on ZIKV infected blood, maintained under three temperature conditions (27℃, 30℃, and 33℃), 28and allowed to oviposit three times. Progeny were tested for virus presence at 3, 7, and 14 days 29 after adult emergence. The overall vertical transmission rate was 6.5% (3.9 -9.9). Vertical

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“…VZV (human herpesvirus 3 ATCC VR-1367) was grown in MRC-145 cells [ 76 ]. ZIKV (strains PVRABC59 and lbH30656 were tested) in Vero cells [ 77 ].…”

Section: Methodsmentioning

confidence: 99%

A universal mammalian vaccine cell line substrate

et al. 2017

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Using genome-wide small interfering RNA (siRNA) screens for poliovirus, influenza A virus and rotavirus, we validated the top 6 gene hits PV, RV or IAV to search for host genes that when knocked-down (KD) enhanced virus permissiveness and replication over wild type Vero cells or HEp-2 cells. The enhanced virus replication was tested for 12 viruses and ranged from 2-fold to >1000-fold. There were variations in virus-specific replication (strain differences) across the cell lines examined. Some host genes (CNTD2, COQ9, GCGR, NDUFA9, NEU2, PYCR1, SEC16G, SVOPL, ZFYVE9, and ZNF205) showed that KD resulted in enhanced virus replication. These findings advance platform-enabling vaccine technology, the creation of diagnostic cells substrates, and are informative about the host mechanisms that affect virus replication in mammalian cells.

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Zika Virus: Quantification, Propagation, Detection, and Storage

Cited by 31 publications

References 18 publications

Cephalotaxine inhibits Zika infection by impeding viral replication and stability

Cephalotaxine inhibits Zika infection by impeding viral replication and stability

Vertical transmission of Zika virus in orally-infected Aedes aegypti produces infectious adult progeny

A universal mammalian vaccine cell line substrate

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