Zika virus RNA polymerase chain reaction on the utility channel of a commercial nucleic acid testing system

Abstract

We developed a sensitive ZIKV PCR assay on the cobas omni UC on the cobas 6800 system. The assay can be used for large-scale screening of blood donations for ZIKV or for testing of blood donors returning from areas with ZIKV to avoid temporal deferral. This study also demonstrates that the cobas omni UC on the cobas 6800 system can be used for in-house-developed PCR assays.

Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system

“…The system used in this study fully automates nucleic acid extraction, purification, amplification and detection. We, among others, have previously demonstrated that laboratory-developed tests can be adapted for fully automated PCR platforms such as the cobas 6800 system [6,13]. After brief preparation, clinical samples can be loaded directly into the device; required hands-on time and manual steps are reduced by up to 60% (manual steps reduced from 33 to 14, hands-on time reduced from 74 min to 14 min) compared with Dark blue curves: specific SARS-CoV-2 signals of IVT-RNA at 100,000 copies/mL (1), 10,000 copies/ml (2), 1,000 copies/ml (3) and 100 copies/ml (4).…”

“…After mixing, the 6 mL are transferred to the reagent cassette. The Mmx cassette is delivered with a full-process control: it is preloaded with an internal control (IC) RNA together with primers and probe of the IC detection assay by default [6]. Instrument settings in the cobas mni Utility Channel software (Roche, Los Gatos, US) and the temperature profile used for the RT-PCR reaction are summarised in Table 1.…”

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

“…Commercial nucleic acid testing devices for detection [217][218][219][220].Such as the instrument-free, disposable PCR testing kit developed by Visby Medical Inc, which allows for on-demand testing with rapid results [221]. Although currently authorized for laboratory testing, their device simplifies the testing process by collecting a sample and placing it in The fully automated and enclosed nature of the extraction, amplification, and detection processes significantly reduces testing time and improves testing efficiency.…”